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Protein

Protein Wnt-8b

Gene

WNT8B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Ligand for members of the frizzled family of seven transmembrane receptors. May play an important role in the development and differentiation of certain forebrain structures, notably the hippocampus.1 Publication

GO - Molecular functioni

GO - Biological processi

  • cell fate commitment Source: GO_Central
  • cellular response to retinoic acid Source: UniProtKB
  • determination of dorsal identity Source: BHF-UCL
  • gastrulation Source: BHF-UCL
  • nervous system development Source: ProtInc
  • neuron differentiation Source: UniProtKB
  • positive regulation of canonical Wnt signaling pathway Source: Reactome
  • response to estradiol Source: BHF-UCL
  • response to retinoic acid Source: BHF-UCL
  • signal transduction Source: ProtInc
  • Wnt signaling pathway Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Wnt signaling pathway

Enzyme and pathway databases

ReactomeiREACT_163710. WNT ligand biogenesis and trafficking.
REACT_18372. Class B/2 (Secretin family receptors).
REACT_264034. disassembly of the destruction complex and recruitment of AXIN to the membrane.
REACT_264596. TCF dependent signaling in response to WNT.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Wnt-8b
Gene namesi
Name:WNT8B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:12789. WNT8B.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA37390.

Polymorphism and mutation databases

BioMutaiWNT8B.
DMDMi66774217.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Chaini23 – 351329Protein Wnt-8bPRO_0000041450Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi54 ↔ 65By similarity
Glycosylationi103 – 1031N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi104 ↔ 112By similarity
Disulfide bondi114 ↔ 132By similarity
Disulfide bondi180 ↔ 194By similarity
Disulfide bondi182 ↔ 189By similarity
Lipidationi186 – 1861O-palmitoleyl serineBy similarity
Disulfide bondi256 ↔ 294By similarity
Glycosylationi259 – 2591N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi272 ↔ 287By similarity
Disulfide bondi291 ↔ 333By similarity
Disulfide bondi309 ↔ 324By similarity
Disulfide bondi311 ↔ 321By similarity
Disulfide bondi316 ↔ 317By similarity

Post-translational modificationi

Palmitoleylation is required for efficient binding to frizzled receptors (By similarity). Depalmitoleylation leads to Wnt signaling pathway inhibition (By similarity).By similarity
Proteolytic processing by TIKI1 and TIKI2 promotes oxidation and formation of large disulfide-bond oligomers, leading to inactivation of WNT8B.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein

Proteomic databases

PaxDbiQ93098.
PRIDEiQ93098.

PTM databases

PhosphoSiteiQ93098.

Expressioni

Tissue specificityi

Expression is restricted to the brain, and more specifically to the forebrain.1 Publication

Gene expression databases

BgeeiQ93098.
CleanExiHS_WNT8B.
GenevisibleiQ93098. HS.

Organism-specific databases

HPAiHPA036570.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000340677.

Structurei

3D structure databases

ProteinModelPortaliQ93098.
SMRiQ93098. Positions 31-334.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Wnt family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG277037.
GeneTreeiENSGT00690000101857.
HOGENOMiHOG000039529.
HOVERGENiHBG001595.
InParanoidiQ93098.
KOiK00714.
OMAiRCEQCRK.
OrthoDBiEOG7C8GJ8.
PhylomeDBiQ93098.
TreeFamiTF105310.

Family and domain databases

InterProiIPR005817. Wnt.
IPR013301. Wnt8.
IPR018161. Wnt_CS.
[Graphical view]
PANTHERiPTHR12027. PTHR12027. 1 hit.
PTHR12027:SF94. PTHR12027:SF94. 1 hit.
PfamiPF00110. wnt. 1 hit.
[Graphical view]
PRINTSiPR01892. WNT8PROTEIN.
PR01349. WNTPROTEIN.
SMARTiSM00097. WNT1. 1 hit.
[Graphical view]
PROSITEiPS00246. WNT1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q93098-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFLSKPSVYI CLFTCVLQLS HSWSVNNFLM TGPKAYLIYS SSVAAGAQSG
60 70 80 90 100
IEECKYQFAW DRWNCPERAL QLSSHGGLRS ANRETAFVHA ISSAGVMYTL
110 120 130 140 150
TRNCSLGDFD NCGCDDSRNG QLGGQGWLWG GCSDNVGFGE AISKQFVDAL
160 170 180 190 200
ETGQDARAAM NLHNNEAGRK AVKGTMKRTC KCHGVSGSCT TQTCWLQLPE
210 220 230 240 250
FREVGAHLKE KYHAALKVDL LQGAGNSAAG RGAIADTFRS ISTRELVHLE
260 270 280 290 300
DSPDYCLENK TLGLLGTEGR ECLRRGRALG RWERRSCRRL CGDCGLAVEE
310 320 330 340 350
RRAETVSSCN CKFHWCCAVR CEQCRRRVTK YFCSRAERPR GGAAHKPGRK

P
Length:351
Mass (Da):38,721
Last modified:May 24, 2005 - v3
Checksum:iDD242070948813F8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti230 – 2301G → A (PubMed:9536085).Curated
Sequence conflicti230 – 2301G → A (PubMed:8661156).Curated
Sequence conflicti284 – 2841R → L (PubMed:9536085).Curated
Sequence conflicti284 – 2841R → L (PubMed:8661156).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti11 – 111C → S.
Corresponds to variant rs3793771 [ dbSNP | Ensembl ].
VAR_020310
Natural varianti53 – 531E → Q in a colorectal cancer sample; somatic mutation. 1 Publication
VAR_036288

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11094 mRNA. Translation: CAA71968.1.
Y11108 Genomic DNA. Translation: CAA71994.1.
AB073637 mRNA. Translation: BAB83924.1.
AL359759, AL133352 Genomic DNA. Translation: CAH71991.1.
AL133352, AL359759 Genomic DNA. Translation: CAH73565.1.
X91940 mRNA. Translation: CAA63017.1.
CCDSiCCDS7494.1.
RefSeqiNP_003384.2. NM_003393.3.
UniGeneiHs.421281.

Genome annotation databases

EnsembliENST00000343737; ENSP00000340677; ENSG00000075290.
GeneIDi7479.
KEGGihsa:7479.
UCSCiuc001krb.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11094 mRNA. Translation: CAA71968.1.
Y11108 Genomic DNA. Translation: CAA71994.1.
AB073637 mRNA. Translation: BAB83924.1.
AL359759, AL133352 Genomic DNA. Translation: CAH71991.1.
AL133352, AL359759 Genomic DNA. Translation: CAH73565.1.
X91940 mRNA. Translation: CAA63017.1.
CCDSiCCDS7494.1.
RefSeqiNP_003384.2. NM_003393.3.
UniGeneiHs.421281.

3D structure databases

ProteinModelPortaliQ93098.
SMRiQ93098. Positions 31-334.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000340677.

PTM databases

PhosphoSiteiQ93098.

Polymorphism and mutation databases

BioMutaiWNT8B.
DMDMi66774217.

Proteomic databases

PaxDbiQ93098.
PRIDEiQ93098.

Protocols and materials databases

DNASUi7479.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000343737; ENSP00000340677; ENSG00000075290.
GeneIDi7479.
KEGGihsa:7479.
UCSCiuc001krb.3. human.

Organism-specific databases

CTDi7479.
GeneCardsiGC10P102212.
HGNCiHGNC:12789. WNT8B.
HPAiHPA036570.
MIMi601396. gene.
neXtProtiNX_Q93098.
PharmGKBiPA37390.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG277037.
GeneTreeiENSGT00690000101857.
HOGENOMiHOG000039529.
HOVERGENiHBG001595.
InParanoidiQ93098.
KOiK00714.
OMAiRCEQCRK.
OrthoDBiEOG7C8GJ8.
PhylomeDBiQ93098.
TreeFamiTF105310.

Enzyme and pathway databases

ReactomeiREACT_163710. WNT ligand biogenesis and trafficking.
REACT_18372. Class B/2 (Secretin family receptors).
REACT_264034. disassembly of the destruction complex and recruitment of AXIN to the membrane.
REACT_264596. TCF dependent signaling in response to WNT.

Miscellaneous databases

GeneWikiiWNT8B.
GenomeRNAii7479.
NextBioi29296.
PROiQ93098.
SOURCEiSearch...

Gene expression databases

BgeeiQ93098.
CleanExiHS_WNT8B.
GenevisibleiQ93098. HS.

Family and domain databases

InterProiIPR005817. Wnt.
IPR013301. Wnt8.
IPR018161. Wnt_CS.
[Graphical view]
PANTHERiPTHR12027. PTHR12027. 1 hit.
PTHR12027:SF94. PTHR12027:SF94. 1 hit.
PfamiPF00110. wnt. 1 hit.
[Graphical view]
PRINTSiPR01892. WNT8PROTEIN.
PR01349. WNTPROTEIN.
SMARTiSM00097. WNT1. 1 hit.
[Graphical view]
PROSITEiPS00246. WNT1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel mammalian Wnt gene, WNT8B, shows brain-restricted expression in early development, with sharply delimited expression boundaries in the developing forebrain."
    Lako M., Lindsay S., Bullen P., Wilson D.I., Robson S.C., Strachan T.
    Hum. Mol. Genet. 7:813-822(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, TISSUE SPECIFICITY.
  2. "Up-regulation of WNT8B mRNA in human gastric cancer."
    Saitoh T., Mine T., Katoh M.
    Int. J. Oncol. 20:343-348(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Fetal brain.
  3. "The DNA sequence and comparative analysis of human chromosome 10."
    Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J.
    , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
    Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "Isolation and characterization of WNT8B, a novel human Wnt gene that maps to 10q24."
    Lako M., Strachan T., Curtis A.R.J., Lindsay S.
    Genomics 35:386-388(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 23-317.
  5. Cited for: VARIANT [LARGE SCALE ANALYSIS] GLN-53.

Entry informationi

Entry nameiWNT8B_HUMAN
AccessioniPrimary (citable) accession number: Q93098
Secondary accession number(s): O00771, Q5VX55, Q8WYK9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 24, 2005
Last modified: July 22, 2015
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.