Q93097 (WNT2B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein Wnt-2b

Alternative name(s):
Protein Wnt-13

Gene names

Name:	WNT2B
Synonyms:	WNT13

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	391 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. May be involved in normal development or differentiation as well as in carcinogenesis.
Subcellular location	Secreted › extracellular space › extracellular matrix.
Tissue specificity	Isoform 1 is expressed in adult heart, brain, placenta, lung, prostate, testis, ovary, small intestine and colon. In the adult brain, it is mainly found in the caudate nucleus, subthalamic nucleus and thalamus. Also detected in fetal brain, lung and kidney. Isoform 2 is expressed in fetal brain, fetal lung, fetal kidney, caudate nucleus, testis and cancer cell lines.
Post-translational modification	Palmitoylation at Ser-243 is required for efficient binding to frizzled receptors. It is also required for subsequent palmitoylation at Cys-107. Palmitoylation is necessary for proper trafficking to cell surface By similarity.
Sequence similarities	Belongs to the Wnt family.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Cellular component	Extracellular matrix Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Molecular function	Developmental protein
PTM	Glycoprotein Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Wnt receptor signaling pathway, calcium modulating pathway Inferred from Biological aspect of Ancestor. Source: RefGenome canonical Wnt receptor signaling pathway Inferred from electronic annotation. Source: Compara chondrocyte differentiation Inferred from expression pattern PubMed 15135146. Source: UniProtKB cornea development in camera-type eye Inferred from sequence or structural similarity PubMed 16258938. Source: BHF-UCL dorsal/ventral axis specification Inferred from Biological aspect of Ancestor. Source: RefGenome forebrain regionalization Inferred from expression pattern PubMed 15164427. Source: UniProtKB hematopoietic stem cell proliferation Inferred from direct assay PubMed 9787155. Source: BHF-UCL iris morphogenesis Inferred from sequence or structural similarity PubMed 16258938. Source: BHF-UCL lens development in camera-type eye Inferred from sequence or structural similarity PubMed 16258938. Source: BHF-UCL lung induction Inferred from Biological aspect of Ancestor. Source: RefGenome male gonad development Inferred from expression pattern PubMed 17848411. Source: UniProtKB mesenchymal-epithelial cell signaling Inferred from electronic annotation. Source: Compara neuron differentiation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of branching involved in ureteric bud morphogenesis Inferred from Biological aspect of Ancestor. Source: RefGenome positive regulation of canonical Wnt receptor signaling pathway Inferred from direct assay PubMed 11741304. Source: UniProtKB
Cellular_component	extracellular space Non-traceable author statement PubMed 15135146. Source: UniProtKB plasma membrane Inferred from Biological aspect of Ancestor. Source: RefGenome proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	frizzled-2 binding Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 2 (identifier: Q93097-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1 (identifier: Q93097-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-61: MLRPGGAEEA...LPARVDTSWW → MLDGLGVVAI...QSAFNKCLQR

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – ?		Potential
	Chain	? – 391		Protein Wnt-2b		PRO_0000041413
Amino acid modifications
	Lipidation	107	1	S-palmitoyl cysteine By similarity
	Lipidation	243	1	O-palmitoyl serine; by PORCN By similarity
	Glycosylation	117	1	N-linked (GlcNAc...) Ref.5
	Glycosylation	283	1	N-linked (GlcNAc...) Ref.5
Natural variations
	Alternative sequence	1 – 61	61	MLRPG…DTSWW → MLDGLGVVAISIFGIQLKTE GSLRTAVPGIPTQSAFNKCL QR in isoform 1.		VSP_006794
Experimental info
	Sequence conflict	151	1	V → I in CAA96283. Ref.1
	Sequence conflict	182	1	D → T in CAA96283. Ref.1
	Sequence conflict	233	1	L → V in CAA96283. Ref.1
	Sequence conflict	287	1	A → T Ref.4
	Sequence conflict	297	1	T → S in CAA96283. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 2 [UniParc]. Last modified June 20, 2001. Version 2. Checksum: BD7BB7F795FB33B1	FASTA	391	43,770
	10 20 30 40 50 60 MLRPGGAEEA AQLPLRRASA PVPVPSPAAP DGSRASARLG LACLLLLLLL TLPARVDTSW 70 80 90 100 110 120 WYIGALGARV ICDNIPGLVS RQRQLCQRYP DIMRSVGEGA REWIRECQHQ FRHHRWNCTT 130 140 150 160 170 180 LDRDHTVFGR VMLRSSREAA FVYAISSAGV VHAITRACSQ GELSVCSCDP YTRGRHHDQR 190 200 210 220 230 240 GDFDWGGCSD NIHYGVRFAK AFVDAKEKRL KDARALMNLH NNRCGRTAVR RFLKLECKCH 250 260 270 280 290 300 GVSGSCTLRT CWRALSDFRR TGDYLRRRYD GAVQVMATQD GANFTAARQG YRRATRTDLV 310 320 330 340 350 360 YFDNSPDYCV LDKAAGSLGT AGRVCSKTSK GTDGCEIMCC GRGYDTTRVT RVTQCECKFH 370 380 390 WCCAVRCKEC RNTVDVHTCK APKKAEWLDQ T
	Isoform 1 [UniParc]. Checksum: 9D8820B9E42ECA29	FASTA	372	41,830
	10 20 30 40 50 60 MLDGLGVVAI SIFGIQLKTE GSLRTAVPGI PTQSAFNKCL QRYIGALGAR VICDNIPGLV 70 80 90 100 110 120 SRQRQLCQRY PDIMRSVGEG AREWIRECQH QFRHHRWNCT TLDRDHTVFG RVMLRSSREA 130 140 150 160 170 180 AFVYAISSAG VVHAITRACS QGELSVCSCD PYTRGRHHDQ RGDFDWGGCS DNIHYGVRFA 190 200 210 220 230 240 KAFVDAKEKR LKDARALMNL HNNRCGRTAV RRFLKLECKC HGVSGSCTLR TCWRALSDFR 250 260 270 280 290 300 RTGDYLRRRY DGAVQVMATQ DGANFTAARQ GYRRATRTDL VYFDNSPDYC VLDKAAGSLG 310 320 330 340 350 360 TAGRVCSKTS KGTDGCEIMC CGRGYDTTRV TRVTQCECKF HWCCAVRCKE CRNTVDVHTC 370 KAPKKAEWLD QT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, expression and chromosomal localization of Wnt-13, a novel member of the Wnt gene family." Katoh M., Hirai M., Sugimura T., Terada M. Oncogene 13:873-876(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Alternative splicing of the WNT-2B/WNT-13 gene." Katoh M., Kirikoshi H., Saitoh T., Sagara N., Koike J. Biochem. Biophys. Res. Commun. 275:209-216(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
[3]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Isolation of two novel WNT genes, WNT14 and WNT15, one of which (WNT15) is closely linked to WNT3 on human chromosome 17q21." Bergstein I., Eisenberg L.M., Bhalerao J., Jenkins N.A., Copeland N.G., Osborne M.P., Bowcock A.M., Brown A.M.C. Genomics 46:450-458(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 243-359.
[5]	"Identification of N-linked glycoproteins in human milk by hydrophilic interaction liquid chromatography and mass spectrometry." Picariello G., Ferranti P., Mamone G., Roepstorff P., Addeo F. Proteomics 8:3833-3847(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-117 AND ASN-283, MASS SPECTROMETRY. Tissue: Milk.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z71621 mRNA. Translation: CAA96283.1. AB045116 mRNA. Translation: BAB11984.1. AB045117 mRNA. Translation: BAB11985.1. AL354760, AL109932 Genomic DNA. Translation: CAI22309.1. AL109932, AL354760 Genomic DNA. Translation: CAI22706.1. AF028701 mRNA. Translation: AAC39552.1.
IPI	IPI00004121. IPI00215680.
PIR	T09612.
RefSeq	NP_004176.2. NM_004185.3. NP_078613.1. NM_024494.2.
UniGene	Hs.258575.
3D structure databases
ProteinModelPortal	Q93097.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000358698.
PTM databases
PhosphoSite	Q93097.
Polymorphism databases
DMDM	14424481.
Proteomic databases
PaxDb	Q93097.
PRIDE	Q93097.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000369684; ENSP00000358698; ENSG00000134245. ENST00000369686; ENSP00000358700; ENSG00000134245.
GeneID	7482.
KEGG	hsa:7482.
UCSC	uc001eca.3. human. uc001ecb.3. human.
Organism-specific databases
CTD	7482.
GeneCards	GC01P113009.
HGNC	HGNC:12781. WNT2B.
MIM	601968. gene.
neXtProt	NX_Q93097.
PharmGKB	PA37382.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG284879.
HOGENOM	HOG000039529.
HOVERGEN	HBG001595.
InParanoid	Q93097.
KO	K00182.
OMA	PDIMQAI.
OrthoDB	EOG4255SV.
PhylomeDB	Q93097.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
ArrayExpress	Q93097.
Bgee	Q93097.
CleanEx	HS_WNT2B.
Genevestigator	Q93097.
GermOnline	ENSG00000134245. Homo sapiens.
Family and domain databases
InterPro	IPR005817. Wnt. IPR009140. Wnt2. IPR018161. Wnt_CS. [Graphical view]
PANTHER	PTHR12027. PTHR12027. 1 hit. PTHR12027:SF21. PTHR12027:SF21. 1 hit.
Pfam	PF00110. wnt. 1 hit. [Graphical view]
PRINTS	PR01842. WNT2PROTEIN. PR01349. WNTPROTEIN.
SMART	SM00097. WNT1. 1 hit. [Graphical view]
PROSITE	PS00246. WNT1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	7482.
NextBio	29308.
SOURCE	Search...

Entry information

Entry name

WNT2B_HUMAN

Accession

Primary (citable) accession number: Q93097
Secondary accession number(s): O14903 , Q5TEI2, Q9HDC1, Q9HDC2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	June 20, 2001
Last modified:	April 3, 2013
This is version 112 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.