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Q93097 (WNT2B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein Wnt-2b
Alternative name(s):
Protein Wnt-13
Gene names
Name:WNT2B
Synonyms:WNT13
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length391 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. May be involved in normal development or differentiation as well as in carcinogenesis.

Subcellular location

Secretedextracellular spaceextracellular matrix.

Tissue specificity

Isoform 1 is expressed in adult heart, brain, placenta, lung, prostate, testis, ovary, small intestine and colon. In the adult brain, it is mainly found in the caudate nucleus, subthalamic nucleus and thalamus. Also detected in fetal brain, lung and kidney. Isoform 2 is expressed in fetal brain, fetal lung, fetal kidney, caudate nucleus, testis and cancer cell lines.

Post-translational modification

Palmitoylation at Ser-243 is required for efficient binding to frizzled receptors. It is also required for subsequent palmitoylation at Cys-107. Palmitoylation is necessary for proper trafficking to cell surface By similarity.

Sequence similarities

Belongs to the Wnt family.

Ontologies

Keywords
   Biological processWnt signaling pathway
   Cellular componentExtracellular matrix
Secreted
   Coding sequence diversityAlternative splicing
   DomainSignal
   Molecular functionDevelopmental protein
   PTMGlycoprotein
Lipoprotein
Palmitate
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processWnt signaling pathway

Inferred from mutant phenotype PubMed 15135146. Source: UniProtKB

canonical Wnt signaling pathway

Inferred from electronic annotation. Source: Ensembl

cell fate commitment

Inferred from Biological aspect of Ancestor. Source: RefGenome

chondrocyte differentiation

Inferred from expression pattern PubMed 15135146. Source: UniProtKB

cornea development in camera-type eye

Inferred from sequence or structural similarity PubMed 16258938. Source: BHF-UCL

forebrain regionalization

Inferred from expression pattern PubMed 15164427. Source: UniProtKB

hematopoietic stem cell proliferation

Inferred from direct assay PubMed 9787155. Source: BHF-UCL

iris morphogenesis

Inferred from sequence or structural similarity PubMed 16258938. Source: BHF-UCL

lens development in camera-type eye

Inferred from sequence or structural similarity PubMed 16258938. Source: BHF-UCL

lung induction

Inferred from electronic annotation. Source: Ensembl

male gonad development

Inferred from expression pattern PubMed 17848411. Source: UniProtKB

mesenchymal-epithelial cell signaling

Inferred from electronic annotation. Source: Ensembl

neuron differentiation

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of branching involved in ureteric bud morphogenesis

Inferred from electronic annotation. Source: Ensembl

positive regulation of canonical Wnt signaling pathway

Inferred from direct assay PubMed 11741304. Source: UniProtKB

   Cellular_componentextracellular region

Traceable author statement. Source: Reactome

extracellular space

Non-traceable author statement PubMed 15135146. Source: UniProtKB

proteinaceous extracellular matrix

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionfrizzled binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 2 (identifier: Q93097-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 1 (identifier: Q93097-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-61: MLRPGGAEEA...LPARVDTSWW → MLDGLGVVAI...QSAFNKCLQR

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – ? Potential
Chain? – 391Protein Wnt-2bPRO_0000041413

Amino acid modifications

Lipidation1071S-palmitoyl cysteine By similarity
Lipidation2431O-palmitoyl serine; by PORCN By similarity
Glycosylation1171N-linked (GlcNAc...) Ref.5
Glycosylation2831N-linked (GlcNAc...) Ref.5

Natural variations

Alternative sequence1 – 6161MLRPG…DTSWW → MLDGLGVVAISIFGIQLKTE GSLRTAVPGIPTQSAFNKCL QR in isoform 1.
VSP_006794

Experimental info

Sequence conflict1511V → I in CAA96283. Ref.1
Sequence conflict1821D → T in CAA96283. Ref.1
Sequence conflict2331L → V in CAA96283. Ref.1
Sequence conflict2871A → T Ref.4
Sequence conflict2971T → S in CAA96283. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 2 [UniParc].

Last modified June 20, 2001. Version 2.
Checksum: BD7BB7F795FB33B1

FASTA39143,770
        10         20         30         40         50         60 
MLRPGGAEEA AQLPLRRASA PVPVPSPAAP DGSRASARLG LACLLLLLLL TLPARVDTSW 

        70         80         90        100        110        120 
WYIGALGARV ICDNIPGLVS RQRQLCQRYP DIMRSVGEGA REWIRECQHQ FRHHRWNCTT 

       130        140        150        160        170        180 
LDRDHTVFGR VMLRSSREAA FVYAISSAGV VHAITRACSQ GELSVCSCDP YTRGRHHDQR 

       190        200        210        220        230        240 
GDFDWGGCSD NIHYGVRFAK AFVDAKEKRL KDARALMNLH NNRCGRTAVR RFLKLECKCH 

       250        260        270        280        290        300 
GVSGSCTLRT CWRALSDFRR TGDYLRRRYD GAVQVMATQD GANFTAARQG YRRATRTDLV 

       310        320        330        340        350        360 
YFDNSPDYCV LDKAAGSLGT AGRVCSKTSK GTDGCEIMCC GRGYDTTRVT RVTQCECKFH 

       370        380        390 
WCCAVRCKEC RNTVDVHTCK APKKAEWLDQ T 

« Hide

Isoform 1 [UniParc].

Checksum: 9D8820B9E42ECA29
Show »

FASTA37241,830

References

« Hide 'large scale' references
[1]"Cloning, expression and chromosomal localization of Wnt-13, a novel member of the Wnt gene family."
Katoh M., Hirai M., Sugimura T., Terada M.
Oncogene 13:873-876(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Alternative splicing of the WNT-2B/WNT-13 gene."
Katoh M., Kirikoshi H., Saitoh T., Sagara N., Koike J.
Biochem. Biophys. Res. Commun. 275:209-216(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
[3]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"Isolation of two novel WNT genes, WNT14 and WNT15, one of which (WNT15) is closely linked to WNT3 on human chromosome 17q21."
Bergstein I., Eisenberg L.M., Bhalerao J., Jenkins N.A., Copeland N.G., Osborne M.P., Bowcock A.M., Brown A.M.C.
Genomics 46:450-458(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 243-359.
[5]"Identification of N-linked glycoproteins in human milk by hydrophilic interaction liquid chromatography and mass spectrometry."
Picariello G., Ferranti P., Mamone G., Roepstorff P., Addeo F.
Proteomics 8:3833-3847(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-117 AND ASN-283.
Tissue: Milk.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z71621 mRNA. Translation: CAA96283.1.
AB045116 mRNA. Translation: BAB11984.1.
AB045117 mRNA. Translation: BAB11985.1.
AL354760, AL109932 Genomic DNA. Translation: CAI22309.1.
AL109932, AL354760 Genomic DNA. Translation: CAI22706.1.
AF028701 mRNA. Translation: AAC39552.1.
PIRT09612.
RefSeqNP_004176.2. NM_004185.3.
NP_078613.1. NM_024494.2.
UniGeneHs.258575.

3D structure databases

ProteinModelPortalQ93097.
SMRQ93097. Positions 95-379.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid113319. 2 interactions.
STRING9606.ENSP00000358698.

PTM databases

PhosphoSiteQ93097.

Polymorphism databases

DMDM14424481.

Proteomic databases

PaxDbQ93097.
PRIDEQ93097.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000369684; ENSP00000358698; ENSG00000134245. [Q93097-1]
ENST00000369686; ENSP00000358700; ENSG00000134245. [Q93097-2]
GeneID7482.
KEGGhsa:7482.
UCSCuc001eca.3. human. [Q93097-2]
uc001ecb.3. human. [Q93097-1]

Organism-specific databases

CTD7482.
GeneCardsGC01P113009.
HGNCHGNC:12781. WNT2B.
HPAHPA060696.
MIM601968. gene.
neXtProtNX_Q93097.
PharmGKBPA37382.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG284879.
HOGENOMHOG000039529.
HOVERGENHBG001595.
InParanoidQ93097.
KOK00182.
OMAPDIMQAI.
PhylomeDBQ93097.
TreeFamTF105310.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.

Gene expression databases

ArrayExpressQ93097.
BgeeQ93097.
CleanExHS_WNT2B.
GenevestigatorQ93097.

Family and domain databases

InterProIPR005817. Wnt.
IPR009140. Wnt2.
IPR018161. Wnt_CS.
[Graphical view]
PANTHERPTHR12027. PTHR12027. 1 hit.
PTHR12027:SF21. PTHR12027:SF21. 1 hit.
PfamPF00110. wnt. 1 hit.
[Graphical view]
PRINTSPR01842. WNT2PROTEIN.
PR01349. WNTPROTEIN.
SMARTSM00097. WNT1. 1 hit.
[Graphical view]
PROSITEPS00246. WNT1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiWNT2B.
GenomeRNAi7482.
NextBio29308.
PROQ93097.
SOURCESearch...

Entry information

Entry nameWNT2B_HUMAN
AccessionPrimary (citable) accession number: Q93097
Secondary accession number(s): O14903 expand/collapse secondary AC list , Q5TEI2, Q9HDC1, Q9HDC2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: June 20, 2001
Last modified: April 16, 2014
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM