Q93086 (P2RX5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
P2X purinoceptor 5
Short name=P2X5

Alternative name(s):
ATP receptor
Purinergic receptor

Gene names

Name:	P2RX5
Synonyms:	P2X5

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	422 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for ATP that acts as a ligand-gated ion channel.
Subunit structure	Homo- or heteropolymers By similarity.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Expressed at high levels in brain and immune system.
Sequence similarities	Belongs to the P2X receptor family.
Sequence caution	The sequence AAF43106.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AK307959 differs from that shown. Reason: Frameshift at position 112. The sequence BAD92860.1 differs from that shown. Reason: Frameshift at position 112. The sequence BC028084 differs from that shown. Reason: Frameshift at position 112.

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Molecular function	Ionic channel Ligand-gated ion channel Receptor
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nervous system development Traceable author statement. Source: ProtInc positive regulation of calcium ion transport into cytosol Non-traceable author statement. Source: BHF-UCL positive regulation of calcium-mediated signaling Non-traceable author statement. Source: BHF-UCL
Cellular component	integral to plasma membrane Traceable author statement. Source: ProtInc
Molecular function	ATP binding Non-traceable author statement. Source: BHF-UCL extracellular ATP-gated cation channel activity Non-traceable author statement. Source: BHF-UCL purinergic nucleotide receptor activity Non-traceable author statement. Source: BHF-UCL
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q93086-3)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q93086-1)

Also known as: A;

The sequence of this isoform differs from the canonical sequence as follows:
     205-206: KS → N

Isoform 3 (identifier: Q93086-2)

Also known as: B;

The sequence of this isoform differs from the canonical sequence as follows:
     97-120: Missing.
     205-206: KS → N

Isoform 4 (identifier: Q93086-4)

The sequence of this isoform differs from the canonical sequence as follows:
     421-422: ST → LRTPSASPLHQE

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 422	422	P2X purinoceptor 5		PRO_0000161555
Regions
	Topological domain	1 – 30	30	Cytoplasmic Potential
	Transmembrane	31 – 51	21	Helical; Name=1; Potential
	Topological domain	52 – 319	268	Extracellular Potential
	Transmembrane	320 – 340	21	Helical; Name=2; Potential
	Topological domain	341 – 422	82	Cytoplasmic Potential
Amino acid modifications
	Modified residue	55	1	Phosphotyrosine Ref.8
	Glycosylation	77	1	N-linked (GlcNAc...) Potential
	Glycosylation	202	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	97 – 120	24	Missing in isoform 3.		VSP_004503
	Alternative sequence	205 – 206	2	KS → N in isoform 2 and isoform 3.		VSP_035587
	Alternative sequence	421 – 422	2	ST → LRTPSASPLHQE in isoform 4.		VSP_035588
Experimental info
	Sequence conflict	96	1	Q → E in AAB08577. Ref.1
	Sequence conflict	97 – 98	2	GE → EK in AAB08576. Ref.1
	Sequence conflict	156	1	E → G in AAC51931. Ref.2
	Sequence conflict	237 – 238	2	VI → IV in AAC51931. Ref.2
	Sequence conflict	251	1	E → R in AAC51931. Ref.2
	Sequence conflict	330	1	F → S in AAB08576. Ref.1
	Sequence conflict	330	1	F → S in AAB08577. Ref.1
	Sequence conflict	352	1	E → Q in AAB08576. Ref.1
	Sequence conflict	352	1	E → Q in AAB08577. Ref.1
	Sequence conflict	399 – 400	2	KR → NV in AAB08576. Ref.1
	Sequence conflict	399 – 400	2	KR → NV in AAB08577. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 14, 2008. Version 4. Checksum: B122027A23B2A240	FASTA	422	47,205
	10 20 30 40 50 60 MGQAGCKGLC LSLFDYKTEK YVIAKNKKVG LLYRLLQASI LAYLVVWVFL IKKGYQDVDT 70 80 90 100 110 120 SLQSAVITKV KGVAFTNTSD LGQRIWDVAD YVIPAQGENV FFVVTNLIVT PNQRQNVCAE 130 140 150 160 170 180 NEGIPDGACS KDSDCHAGEA VTAGNGVKTG RCLRRENLAR GTCEIFAWCP LETSSRPEEP 190 200 210 220 230 240 FLKEAEDFTI FIKNHIRFPK FNFSKSNVMD VKDRSFLKSC HFGPKNHYCP IFRLGSVIRW 250 260 270 280 290 300 AGSDFQDIAL EGGVIGINIE WNCDLDKAAS ECHPHYSFSR LDNKLSKSVS SGYNFRFARY 310 320 330 340 350 360 YRDAAGVEFR TLMKAYGIRF DVMVNGKGAF FCDLVLIYLI KKREFYRDKK YEEVRGLEDS 370 380 390 400 410 420 SQEAEDEASG LGLSEQLTSG PGLLGMPEQQ ELQEPPEAKR GSSSQKGNGS VCPQLLEPHR ST
	Isoform 2 (A) [UniParc]. Checksum: 6B47307AA14C2A6C	FASTA	421	47,104
	10 20 30 40 50 60 MGQAGCKGLC LSLFDYKTEK YVIAKNKKVG LLYRLLQASI LAYLVVWVFL IKKGYQDVDT 70 80 90 100 110 120 SLQSAVITKV KGVAFTNTSD LGQRIWDVAD YVIPAQGENV FFVVTNLIVT PNQRQNVCAE 130 140 150 160 170 180 NEGIPDGACS KDSDCHAGEA VTAGNGVKTG RCLRRENLAR GTCEIFAWCP LETSSRPEEP 190 200 210 220 230 240 FLKEAEDFTI FIKNHIRFPK FNFSNNVMDV KDRSFLKSCH FGPKNHYCPI FRLGSVIRWA 250 260 270 280 290 300 GSDFQDIALE GGVIGINIEW NCDLDKAASE CHPHYSFSRL DNKLSKSVSS GYNFRFARYY 310 320 330 340 350 360 RDAAGVEFRT LMKAYGIRFD VMVNGKGAFF CDLVLIYLIK KREFYRDKKY EEVRGLEDSS 370 380 390 400 410 420 QEAEDEASGL GLSEQLTSGP GLLGMPEQQE LQEPPEAKRG SSSQKGNGSV CPQLLEPHRS T
	Isoform 3 (B) [UniParc]. Checksum: C3DB8AB55858CEB0	FASTA	397	44,430
	10 20 30 40 50 60 MGQAGCKGLC LSLFDYKTEK YVIAKNKKVG LLYRLLQASI LAYLVVWVFL IKKGYQDVDT 70 80 90 100 110 120 SLQSAVITKV KGVAFTNTSD LGQRIWDVAD YVIPAQNEGI PDGACSKDSD CHAGEAVTAG 130 140 150 160 170 180 NGVKTGRCLR RENLARGTCE IFAWCPLETS SRPEEPFLKE AEDFTIFIKN HIRFPKFNFS 190 200 210 220 230 240 NNVMDVKDRS FLKSCHFGPK NHYCPIFRLG SVIRWAGSDF QDIALEGGVI GINIEWNCDL 250 260 270 280 290 300 DKAASECHPH YSFSRLDNKL SKSVSSGYNF RFARYYRDAA GVEFRTLMKA YGIRFDVMVN 310 320 330 340 350 360 GKGAFFCDLV LIYLIKKREF YRDKKYEEVR GLEDSSQEAE DEASGLGLSE QLTSGPGLLG 370 380 390 MPEQQELQEP PEAKRGSSSQ KGNGSVCPQL LEPHRST
	Isoform 4 [UniParc]. Checksum: 53AD66E4F75A69E3	FASTA	432	48,334
	10 20 30 40 50 60 MGQAGCKGLC LSLFDYKTEK YVIAKNKKVG LLYRLLQASI LAYLVVWVFL IKKGYQDVDT 70 80 90 100 110 120 SLQSAVITKV KGVAFTNTSD LGQRIWDVAD YVIPAQGENV FFVVTNLIVT PNQRQNVCAE 130 140 150 160 170 180 NEGIPDGACS KDSDCHAGEA VTAGNGVKTG RCLRRENLAR GTCEIFAWCP LETSSRPEEP 190 200 210 220 230 240 FLKEAEDFTI FIKNHIRFPK FNFSKSNVMD VKDRSFLKSC HFGPKNHYCP IFRLGSVIRW 250 260 270 280 290 300 AGSDFQDIAL EGGVIGINIE WNCDLDKAAS ECHPHYSFSR LDNKLSKSVS SGYNFRFARY 310 320 330 340 350 360 YRDAAGVEFR TLMKAYGIRF DVMVNGKGAF FCDLVLIYLI KKREFYRDKK YEEVRGLEDS 370 380 390 400 410 420 SQEAEDEASG LGLSEQLTSG PGLLGMPEQQ ELQEPPEAKR GSSSQKGNGS VCPQLLEPHR 430 LRTPSASPLH QE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of human P2X purinoceptor new subtype (P2X5)." Tokuyama Y., Mereu L., Chen X., Rouard M., Bell G.I. Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3).
[2]	"Primary structure and expression of a naturally truncated human P2X ATP receptor subunit from brain and immune system." Le K.-T., Paquet M., Nouel D., Babinski K., Seguela P. FEBS Lett. 418:195-199(1997) [PubMed: 9414125] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Brain.
[3]	"The genomic region encompassing the nephropathic cystinosis gene (CTNS): complete sequencing of a 200-kb segment and discovery of a novel gene within the common cystinosis-causing deletion." Touchman J.W., Anikster Y., Dietrich N.L., Maduro V.V.B., McDowell G., Shotelersuk V., Bouffard G.G., Beckstrom-Sternberg S.M., Gahl W.A., Green E.D. Genome Res. 10:165-173(2000) [PubMed: 10673275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	Yu W., Gibbs R.A. Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Brain.
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Erythroleukemia and Mesangial cell.
[6]	Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4). Tissue: Spleen.
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Leukocyte and Lung.
[8]	"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra." Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D. J. Proteome Res. 6:4150-4162(2007) [PubMed: 17924679] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-55, MASS SPECTROMETRY. Tissue: Cervix carcinoma.

Web resources

Wikipedia

P2X receptor entry

Cross-references

Sequence databases
EMBL GenBank DDBJ	U49395 mRNA. Translation: AAB08576.1. U49396 mRNA. Translation: AAB08577.1. AF016709 mRNA. Translation: AAC51931.1. AF168787 Genomic DNA. Translation: AAF43105.1. AF168787 Genomic DNA. Translation: AAF43106.1. Sequence problems. AF070573 mRNA. Translation: AAC28645.1. AK290889 mRNA. Translation: BAF83578.1. AK307959 mRNA. No translation available. AB209623 mRNA. Translation: BAD92860.1. Frameshift. BC028084 mRNA. No translation available. BC039015 mRNA. Translation: AAH39015.1.
IPI	IPI00217666. IPI00789729. IPI00873205. IPI00914060.
RefSeq	NP_001191448.1. NM_001204519.1. NP_001191449.1. NM_001204520.1. NP_002552.2. NM_002561.3. NP_778255.1. NM_175080.2.
UniGene	Hs.408615.
3D structure databases
ProteinModelPortal	Q93086.
SMR	Q93086. Positions 34-331.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q93086. 1 interaction.
STRING	Q93086.
Polymorphism databases
DMDM	209572778.
Proteomic databases
PRIDE	Q93086.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000225328; ENSP00000225328; ENSG00000083454.
GeneID	5026.
KEGG	hsa:5026.
UCSC	uc002fwh.1. human. uc002fwj.1. human.
Organism-specific databases
CTD	5026.
GeneCards	GC17M003575.
HGNC	HGNC:8536. P2RX5.
HPA	HPA021948.
MIM	602836. gene.
neXtProt	NX_Q93086.
PharmGKB	PA32865.
GenAtlas	Search...
Phylogenomic databases
GeneTree	ENSGT00390000016028.
HOVERGEN	HBG053086.
OrthoDB	EOG46Q6SQ.
PhylomeDB	Q93086.
Gene expression databases
ArrayExpress	Q93086.
Bgee	Q93086.
CleanEx	HS_P2RX5.
Genevestigator	Q93086.
GermOnline	ENSG00000083454. Homo sapiens.
Family and domain databases
InterPro	IPR003048. P2X5_purnocptor. IPR001429. P2X_purnocptor. [Graphical view]
KO	K05219.
PANTHER	PTHR10125. ATP_P2X_rcpt. 1 hit. PTHR10125:SF12. P2X5_purnocptor. 1 hit.
Pfam	PF00864. P2X_receptor. 2 hits. [Graphical view]
PRINTS	PR01312. P2X5RECEPTOR. PR01307. P2XRECEPTOR.
TIGRFAMs	TIGR00863. P2X. 1 hit.
PROSITE	PS01212. P2X_RECEPTOR. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	19350.
SOURCE	Search...

Entry information

Entry name

P2RX5_HUMAN

Accession

Primary (citable) accession number: Q93086
Secondary accession number(s): O43450 , O75540, Q59F38, Q8IXW4, Q93087, Q9NZV0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	October 14, 2008
Last modified:	January 25, 2012
This is version 101 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.