Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q93074

- MED12_HUMAN

UniProt

Q93074 - MED12_HUMAN

Protein

Mediator of RNA polymerase II transcription subunit 12

Gene

MED12

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 145 (01 Oct 2014)
      Sequence version 4 (14 Oct 2008)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway.3 Publications

    GO - Molecular functioni

    1. chromatin binding Source: Ensembl
    2. ligand-dependent nuclear receptor transcription coactivator activity Source: UniProtKB
    3. protein binding Source: IntAct
    4. protein C-terminus binding Source: UniProtKB
    5. protein domain specific binding Source: UniProtKB
    6. receptor activity Source: UniProtKB
    7. RNA polymerase II distal enhancer sequence-specific DNA binding Source: Ensembl
    8. RNA polymerase II transcription cofactor activity Source: UniProtKB
    9. RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription Source: Ensembl
    10. thyroid hormone receptor binding Source: UniProtKB
    11. transcription coactivator activity Source: MGI
    12. transcription cofactor activity Source: UniProtKB
    13. vitamin D receptor binding Source: UniProtKB

    GO - Biological processi

    1. androgen receptor signaling pathway Source: UniProtKB
    2. axis elongation involved in somitogenesis Source: Ensembl
    3. canonical Wnt signaling pathway Source: Ensembl
    4. gene expression Source: Reactome
    5. heart development Source: Ensembl
    6. intracellular steroid hormone receptor signaling pathway Source: UniProtKB
    7. negative regulation of Wnt signaling pathway Source: UniProtKB
    8. neural tube closure Source: Ensembl
    9. oligodendrocyte development Source: Ensembl
    10. positive regulation of transcription, DNA-templated Source: UniProtKB
    11. positive regulation of transcription from RNA polymerase II promoter Source: MGI
    12. Schwann cell development Source: Ensembl
    13. stem cell maintenance Source: Ensembl
    14. transcription initiation from RNA polymerase II promoter Source: UniProtKB
    15. Wnt signaling pathway, planar cell polarity pathway Source: Ensembl

    Keywords - Molecular functioni

    Activator, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Enzyme and pathway databases

    ReactomeiREACT_116145. PPARA activates gene expression.
    REACT_12627. Generic Transcription Pathway.
    REACT_27161. Transcriptional regulation of white adipocyte differentiation.
    SignaLinkiQ93074.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mediator of RNA polymerase II transcription subunit 12
    Alternative name(s):
    Activator-recruited cofactor 240 kDa component
    Short name:
    ARC240
    CAG repeat protein 45
    Mediator complex subunit 12
    OPA-containing protein
    Thyroid hormone receptor-associated protein complex 230 kDa component
    Short name:
    Trap230
    Trinucleotide repeat-containing gene 11 protein
    Gene namesi
    Name:MED12
    Synonyms:ARC240, CAGH45, HOPA, KIAA0192, TNRC11, TRAP230
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome X

    Organism-specific databases

    HGNCiHGNC:11957. MED12.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. mediator complex Source: UniProtKB
    2. membrane Source: UniProtKB
    3. nucleoplasm Source: Reactome
    4. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Involvement in diseasei

    Opitz-Kaveggia syndrome (OKS) [MIM:305450]: X-linked disorder characterized by mental retardation, relative macrocephaly, hypotonia and constipation.1 Publication
    Note: The disease is caused by mutations affecting the gene represented in this entry.
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti961 – 9611R → W in OKS. 1 Publication
    VAR_033112
    Lujan-Fryns syndrome (LUJFRYS) [MIM:309520]: Clinically, Lujan-Fryns syndrome can be distinguished from Opitz-Kaveggia syndrome by tall stature, hypernasal voice, hyperextensible digits and high nasal root.1 Publication
    Note: The disease is caused by mutations affecting the gene represented in this entry.
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti1007 – 10071N → S in LUJFRYS. 1 Publication
    VAR_037534
    Ohdo syndrome, X-linked (OHDOX) [MIM:300895]: A syndrome characterized by mental retardation, feeding problems, and distinctive facial appearance with coarse facial features, severe blepharophimosis, ptosis, a bulbous nose, micrognathia and a small mouth. Dental hypoplasia and deafness can be considered as common manifestations of the syndrome. Male patients show cryptorchidism and scrotal hypoplasia.1 Publication
    Note: The disease is caused by mutations affecting the gene represented in this entry.
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti1148 – 11481R → H in OHDOX. 1 Publication
    VAR_069770
    Natural varianti1165 – 11651S → P in OHDOX. 1 Publication
    VAR_069771
    Natural varianti1729 – 17291H → N in OHDOX. 1 Publication
    VAR_069772

    Keywords - Diseasei

    Disease mutation, Mental retardation

    Organism-specific databases

    MIMi300895. phenotype.
    305450. phenotype.
    309520. phenotype.
    Orphaneti293707. Blepharophimosis-intellectual disability syndrome, MKB type.
    93932. FG syndrome type 1.
    776. X-linked intellectual disability with marfanoid habitus.
    777. X-linked non-syndromic intellectual disability.
    PharmGKBiPA36645.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 21772177Mediator of RNA polymerase II transcription subunit 12PRO_0000096359Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei80 – 801N6-acetyllysineBy similarity
    Modified residuei166 – 1661Phosphotyrosine1 Publication
    Modified residuei635 – 6351Phosphoserine4 Publications
    Modified residuei1258 – 12581Phosphoserine2 Publications
    Modified residuei1269 – 12691Phosphoserine1 Publication
    Modified residuei1798 – 17981N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ93074.
    PaxDbiQ93074.
    PRIDEiQ93074.

    PTM databases

    PhosphoSiteiQ93074.

    Expressioni

    Tissue specificityi

    Ubiquitous.1 Publication

    Gene expression databases

    ArrayExpressiQ93074.
    BgeeiQ93074.
    CleanExiHS_MED12.
    GenevestigatoriQ93074.

    Organism-specific databases

    HPAiHPA003184.
    HPA003185.

    Interactioni

    Subunit structurei

    Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Also interacts with CTNNB1 and GLI3.8 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    APLP1P516932EBI-394357,EBI-74648
    APLP2Q064812EBI-394357,EBI-79306
    APPP050672EBI-394357,EBI-77613
    MED4Q9NPJ65EBI-394357,EBI-394607
    TGFBR2P371733EBI-394357,EBI-296151

    Protein-protein interaction databases

    BioGridi115293. 62 interactions.
    DIPiDIP-31459N.
    IntActiQ93074. 27 interactions.
    STRINGi9606.ENSP00000363193.

    Structurei

    3D structure databases

    ProteinModelPortaliQ93074.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1616 – 2051436Interaction with CTNNB1 and GLI3Add
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi1732 – 177746Pro-richAdd
    BLAST
    Compositional biasi1900 – 2168269Gln-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the Mediator complex subunit 12 family.Curated

    Phylogenomic databases

    eggNOGiNOG319131.
    HOGENOMiHOG000231423.
    HOVERGENiHBG052447.
    InParanoidiQ93074.
    KOiK15162.
    OMAiTVDMQSN.
    OrthoDBiEOG7CRTNZ.
    PhylomeDBiQ93074.
    TreeFamiTF324178.

    Family and domain databases

    InterProiIPR019035. Mediator_Med12.
    IPR021989. Mediator_Med12_catenin-bd.
    IPR021990. Mediator_Med12_LCEWAV.
    [Graphical view]
    PfamiPF09497. Med12. 1 hit.
    PF12145. Med12-LCEWAV. 1 hit.
    PF12144. Med12-PQL. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q93074-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAAFGILSYE HRPLKRPRLG PPDVYPQDPK QKEDELTALN VKQGFNNQPA     50
    VSGDEHGSAK NVSFNPAKIS SNFSSIIAEK LRCNTLPDTG RRKPQVNQKD 100
    NFWLVTARSQ SAINTWFTDL AGTKPLTQLA KKVPIFSKKE EVFGYLAKYT 150
    VPVMRAAWLI KMTCAYYAAI SETKVKKRHV DPFMEWTQII TKYLWEQLQK 200
    MAEYYRPGPA GSGGCGSTIG PLPHDVEVAI RQWDYTEKLA MFMFQDGMLD 250
    RHEFLTWVLE CFEKIRPGED ELLKLLLPLL LRYSGEFVQS AYLSRRLAYF 300
    CTRRLALQLD GVSSHSSHVI SAQSTSTLPT TPAPQPPTSS TPSTPFSDLL 350
    MCPQHRPLVF GLSCILQTIL LCCPSALVWH YSLTDSRIKT GSPLDHLPIA 400
    PSNLPMPEGN SAFTQQVRAK LREIEQQIKE RGQAVEVRWS FDKCQEATAG 450
    FTIGRVLHTL EVLDSHSFER SDFSNSLDSL CNRIFGLGPS KDGHEISSDD 500
    DAVVSLLCEW AVSCKRSGRH RAMVVAKLLE KRQAEIEAER CGESEAADEK 550
    GSIASGSLSA PSAPIFQDVL LQFLDTQAPM LTDPRSESER VEFFNLVLLF 600
    CELIRHDVFS HNMYTCTLIS RGDLAFGAPG PRPPSPFDDP ADDPEHKEAE 650
    GSSSSKLEDP GLSESMDIDP SSSVLFEDME KPDFSLFSPT MPCEGKGSPS 700
    PEKPDVEKEV KPPPKEKIEG TLGVLYDQPR HVQYATHFPI PQEESCSHEC 750
    NQRLVVLFGV GKQRDDARHA IKKITKDILK VLNRKGTAET DQLAPIVPLN 800
    PGDLTFLGGE DGQKRRRNRP EAFPTAEDIF AKFQHLSHYD QHQVTAQVSR 850
    NVLEQITSFA LGMSYHLPLV QHVQFIFDLM EYSLSISGLI DFAIQLLNEL 900
    SVVEAELLLK SSDLVGSYTT SLCLCIVAVL RHYHACLILN QDQMAQVFEG 950
    LCGVVKHGMN RSDGSSAERC ILAYLYDLYT SCSHLKNKFG ELFSDFCSKV 1000
    KNTIYCNVEP SESNMRWAPE FMIDTLENPA AHTFTYTGLG KSLSENPANR 1050
    YSFVCNALMH VCVGHHDPDR VNDIAILCAE LTGYCKSLSA EWLGVLKALC 1100
    CSSNNGTCGF NDLLCNVDVS DLSFHDSLAT FVAILIARQC LLLEDLIRCA 1150
    AIPSLLNAAC SEQDSEPGAR LTCRILLHLF KTPQLNPCQS DGNKPTVGIR 1200
    SSCDRHLLAA SQNRIVDGAV FAVLKAVFVL GDAELKGSGF TVTGGTEELP 1250
    EEEGGGGSGG RRQGGRNISV ETASLDVYAK YVLRSICQQE WVGERCLKSL 1300
    CEDSNDLQDP VLSSAQAQRL MQLICYPHRL LDNEDGENPQ RQRIKRILQN 1350
    LDQWTMRQSS LELQLMIKQT PNNEMNSLLE NIAKATIEVF QQSAETGSSS 1400
    GSTASNMPSS SKTKPVLSSL ERSGVWLVAP LIAKLPTSVQ GHVLKAAGEE 1450
    LEKGQHLGSS SRKERDRQKQ KSMSLLSQQP FLSLVLTCLK GQDEQREGLL 1500
    TSLYSQVHQI VNNWRDDQYL DDCKPKQLMH EALKLRLNLV GGMFDTVQRS 1550
    TQQTTEWAML LLEIIISGTV DMQSNNELFT TVLDMLSVLI NGTLAADMSS 1600
    ISQGSMEENK RAYMNLAKKL QKELGERQSD SLEKVRQLLP LPKQTRDVIT 1650
    CEPQGSLIDT KGNKIAGFDS IFKKEGLQVS TKQKISPWDL FEGLKPSAPL 1700
    SWGWFGTVRV DRRVARGEEQ QRLLLYHTHL RPRPRAYYLE PLPLPPEDEE 1750
    PPAPTLLEPE KKAPEPPKTD KPGAAPPSTE ERKKKSTKGK KRSQPATKTE 1800
    DYGMGPGRSG PYGVTVPPDL LHHPNPGSIT HLNYRQGSIG LYTQNQPLPA 1850
    GGPRVDPYRP VRLPMQKLPT RPTYPGVLPT TMTGVMGLEP SSYKTSVYRQ 1900
    QQPAVPQGQR LRQQLQQSQG MLGQSSVHQM TPSSSYGLQT SQGYTPYVSH 1950
    VGLQQHTGPA GTMVPPSYSS QPYQSTHPST NPTLVDPTRH LQQRPSGYVH 2000
    QQAPTYGHGL TSTQRFSHQT LQQTPMISTM TPMSAQGVQA GVRSTAILPE 2050
    QQQQQQQQQQ QQQQQQQQQQ QQQQQQYHIR QQQQQQILRQ QQQQQQQQQQ 2100
    QQQQQQQQQQ QQQQQHQQQQ QQQAAPPQPQ PQSQPQFQRQ GLQQTQQQQQ 2150
    TAALVRQLQQ QLSNTQPQPS TNIFGRY 2177
    Length:2,177
    Mass (Da):243,081
    Last modified:October 14, 2008 - v4
    Checksum:i7492B07BA0F6EA9D
    GO
    Isoform 2 (identifier: Q93074-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1916-1916: Q → QAKI

    Show »
    Length:2,180
    Mass (Da):243,394
    Checksum:i22DB2ABC4A1CCA6B
    GO
    Isoform 3 (identifier: Q93074-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1916-1916: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:2,176
    Mass (Da):242,953
    Checksum:i440A5E60BA3E7273
    GO

    Sequence cautioni

    The sequence AAD22033.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti16 – 161R → RPR in AAD22033. (PubMed:10198638)Curated
    Sequence conflicti397 – 3971Missing AA sequence (PubMed:10235266)Curated
    Sequence conflicti1166 – 11661E → V in AAD44162. (PubMed:10480376)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti961 – 9611R → W in OKS. 1 Publication
    VAR_033112
    Natural varianti1007 – 10071N → S in LUJFRYS. 1 Publication
    VAR_037534
    Natural varianti1148 – 11481R → H in OHDOX. 1 Publication
    VAR_069770
    Natural varianti1165 – 11651S → P in OHDOX. 1 Publication
    VAR_069771
    Natural varianti1392 – 13921Q → R.2 Publications
    Corresponds to variant rs1139013 [ dbSNP | Ensembl ].
    VAR_046672
    Natural varianti1729 – 17291H → N in OHDOX. 1 Publication
    VAR_069772

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1916 – 19161Q → QAKI in isoform 2. 1 PublicationVSP_035520
    Alternative sequencei1916 – 19161Missing in isoform 3. 2 PublicationsVSP_035521

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF117755 mRNA. Translation: AAD22033.1. Different initiation.
    AL590764 Genomic DNA. No translation available.
    D83783 mRNA. Translation: BAA12112.1.
    AF071309 mRNA. Translation: AAC83163.1.
    AF132033 Genomic DNA. Translation: AAD44162.1.
    U80742 mRNA. Translation: AAB91440.1.
    CCDSiCCDS43970.1. [Q93074-1]
    RefSeqiNP_005111.2. NM_005120.2. [Q93074-1]
    XP_005262374.1. XM_005262317.1. [Q93074-2]
    UniGeneiHs.409226.

    Genome annotation databases

    EnsembliENST00000333646; ENSP00000333125; ENSG00000184634. [Q93074-2]
    ENST00000374080; ENSP00000363193; ENSG00000184634. [Q93074-1]
    ENST00000374102; ENSP00000363215; ENSG00000184634. [Q93074-3]
    GeneIDi9968.
    KEGGihsa:9968.
    UCSCiuc004dyy.3. human. [Q93074-1]
    uc004dyz.3. human. [Q93074-3]

    Polymorphism databases

    DMDMi209572775.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF117755 mRNA. Translation: AAD22033.1 . Different initiation.
    AL590764 Genomic DNA. No translation available.
    D83783 mRNA. Translation: BAA12112.1 .
    AF071309 mRNA. Translation: AAC83163.1 .
    AF132033 Genomic DNA. Translation: AAD44162.1 .
    U80742 mRNA. Translation: AAB91440.1 .
    CCDSi CCDS43970.1. [Q93074-1 ]
    RefSeqi NP_005111.2. NM_005120.2. [Q93074-1 ]
    XP_005262374.1. XM_005262317.1. [Q93074-2 ]
    UniGenei Hs.409226.

    3D structure databases

    ProteinModelPortali Q93074.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 115293. 62 interactions.
    DIPi DIP-31459N.
    IntActi Q93074. 27 interactions.
    STRINGi 9606.ENSP00000363193.

    PTM databases

    PhosphoSitei Q93074.

    Polymorphism databases

    DMDMi 209572775.

    Proteomic databases

    MaxQBi Q93074.
    PaxDbi Q93074.
    PRIDEi Q93074.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000333646 ; ENSP00000333125 ; ENSG00000184634 . [Q93074-2 ]
    ENST00000374080 ; ENSP00000363193 ; ENSG00000184634 . [Q93074-1 ]
    ENST00000374102 ; ENSP00000363215 ; ENSG00000184634 . [Q93074-3 ]
    GeneIDi 9968.
    KEGGi hsa:9968.
    UCSCi uc004dyy.3. human. [Q93074-1 ]
    uc004dyz.3. human. [Q93074-3 ]

    Organism-specific databases

    CTDi 9968.
    GeneCardsi GC0XP070338.
    GeneReviewsi MED12.
    HGNCi HGNC:11957. MED12.
    HPAi HPA003184.
    HPA003185.
    MIMi 300188. gene.
    300895. phenotype.
    305450. phenotype.
    309520. phenotype.
    neXtProti NX_Q93074.
    Orphaneti 293707. Blepharophimosis-intellectual disability syndrome, MKB type.
    93932. FG syndrome type 1.
    776. X-linked intellectual disability with marfanoid habitus.
    777. X-linked non-syndromic intellectual disability.
    PharmGKBi PA36645.
    HUGEi Search...
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG319131.
    HOGENOMi HOG000231423.
    HOVERGENi HBG052447.
    InParanoidi Q93074.
    KOi K15162.
    OMAi TVDMQSN.
    OrthoDBi EOG7CRTNZ.
    PhylomeDBi Q93074.
    TreeFami TF324178.

    Enzyme and pathway databases

    Reactomei REACT_116145. PPARA activates gene expression.
    REACT_12627. Generic Transcription Pathway.
    REACT_27161. Transcriptional regulation of white adipocyte differentiation.
    SignaLinki Q93074.

    Miscellaneous databases

    ChiTaRSi MED12. human.
    GeneWikii MED12.
    GenomeRNAii 9968.
    NextBioi 37618.
    PROi Q93074.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q93074.
    Bgeei Q93074.
    CleanExi HS_MED12.
    Genevestigatori Q93074.

    Family and domain databases

    InterProi IPR019035. Mediator_Med12.
    IPR021989. Mediator_Med12_catenin-bd.
    IPR021990. Mediator_Med12_LCEWAV.
    [Graphical view ]
    Pfami PF09497. Med12. 1 hit.
    PF12145. Med12-LCEWAV. 1 hit.
    PF12144. Med12-PQL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identity between TRAP and SMCC complexes indicates novel pathways for the function of nuclear receptors and diverse mammalian activators."
      Ito M., Yuan C.-X., Malik S., Gu W., Fondell J.D., Yamamura S., Fu Z.-Y., Zhang X., Qin J., Roeder R.G.
      Mol. Cell 3:361-370(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 788-802 (ISOFORMS 1/2/3), IDENTIFICATION IN THE TRAP COMPLEX, TISSUE SPECIFICITY, VARIANT ARG-1392.
      Tissue: Cervix carcinoma.
    2. "The DNA sequence of the human X chromosome."
      Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C.
      , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R.
      Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "Prediction of the coding sequences of unidentified human genes. V. The coding sequences of 40 new genes (KIAA0161-KIAA0200) deduced by analysis of cDNA clones from human cell line KG-1."
      Nagase T., Seki N., Ishikawa K., Tanaka A., Nomura N.
      DNA Res. 3:17-24(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 54-2177 (ISOFORM 1), VARIANT ARG-1392.
      Tissue: Bone marrow.
    4. "Association of an X-chromosome dodecamer insertional variant allele with mental retardation."
      Philibert R.A., King B.H., Cook E.H., Lee Y.-H., Stubblefield B., Damschroder-Williams P., Dea C., Palotie A., Tengstrom C., Martin B.M., Ginns E.I.
      Mol. Psychiatry 3:303-309(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 154-2177 (ISOFORM 3).
    5. "The genomic structure and developmental expression patterns of the human OPA-containing gene (HOPA)."
      Philibert R.A., Winfield S.L., Damschroder-Williams P., Tengstrom C., Martin B.M., Ginns E.I.
      Hum. Genet. 105:174-178(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 154-2177 (ISOFORM 3).
    6. "Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex."
      Rachez C., Lemon B.D., Suldan Z., Bromleigh V., Gamble M., Naeaer A.M., Erdjument-Bromage H., Tempst P., Freedman L.P.
      Nature 398:824-828(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 386-418 (ISOFORMS 1/2/3), IDENTIFICATION IN THE ARC COMPLEX.
      Tissue: Cervix carcinoma.
    7. Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1529-2177 (ISOFORM 2).
      Tissue: Brain.
    8. "Composite co-activator ARC mediates chromatin-directed transcriptional activation."
      Naeaer A.M., Beaurang P.A., Zhou S., Abraham S., Solomon W.B., Tjian R.
      Nature 398:828-832(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1674-1682 AND 1771-1782 (ISOFORMS 1/2/3), IDENTIFICATION IN THE ARC COMPLEX.
    9. "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology."
      Sato S., Tomomori-Sato C., Parmely T.J., Florens L., Zybailov B., Swanson S.K., Banks C.A.S., Jin J., Cai Y., Washburn M.P., Conaway J.W., Conaway R.C.
      Mol. Cell 14:685-691(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX, INTERACTION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
    10. "MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription."
      Zhang X., Krutchinsky A., Fukuda A., Chen W., Yamamura S., Chait B.T., Roeder R.G.
      Mol. Cell 19:89-100(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MED1; MED18; MED21; MED28; MED29 AND MED30, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX, ASSOCIATION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
    11. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
      Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
      Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-635, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    12. "Mediator is a transducer of Wnt/beta-catenin signaling."
      Kim S., Xu X., Hecht A., Boyer T.G.
      J. Biol. Chem. 281:14066-14075(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CTNNB1 AND MED30.
    13. "Human Mediator enhances basal transcription by facilitating recruitment of transcription factor IIB during preinitiation complex assembly."
      Baek H.J., Kang Y.K., Roeder R.G.
      J. Biol. Chem. 281:15172-15181(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH MED1 AND MED10.
    14. "Mediator modulates Gli3-dependent Sonic hedgehog signaling."
      Zhou H., Kim S., Ishii S., Boyer T.G.
      Mol. Cell. Biol. 26:8667-8682(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CDK8; CTNNB1 AND GLI3.
    15. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
      Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
      Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-635 AND SER-1258, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    16. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-166, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    17. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-635; SER-1258 AND SER-1269, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    18. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-635, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.
    19. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    20. Cited for: VARIANT LUJFRYS SER-1007.
    21. Cited for: VARIANT OKS TRP-961.
    22. Cited for: VARIANTS OHDOX HIS-1148; PRO-1165 AND ASN-1729.

    Entry informationi

    Entry nameiMED12_HUMAN
    AccessioniPrimary (citable) accession number: Q93074
    Secondary accession number(s): O15410
    , O75557, Q9UHV6, Q9UND7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: October 14, 2008
    Last modified: October 1, 2014
    This is version 145 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome X
      Human chromosome X: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3