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Protein

Ecto-ADP-ribosyltransferase 4

Gene

ART4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

NAD+ + protein-L-arginine = nicotinamide + N(omega)-(ADP-D-ribosyl)-protein-L-arginine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei126NADBy similarity1
Binding sitei206NADBy similarity1
Binding sitei240NADBy similarity1
Active sitei251By similarity1

GO - Molecular functioni

  • NAD(P)+-protein-arginine ADP-ribosyltransferase activity Source: UniProtKB

GO - Biological processi

  • arginine metabolic process Source: UniProtKB
  • protein ADP-ribosylation Source: UniProtKB

Keywordsi

Molecular functionBlood group antigen, Glycosyltransferase, Transferase
LigandNAD, NADP

Enzyme and pathway databases

ReactomeiR-HSA-163125 Post-translational modification: synthesis of GPI-anchored proteins

Names & Taxonomyi

Protein namesi
Recommended name:
Ecto-ADP-ribosyltransferase 4 (EC:2.4.2.31)
Alternative name(s):
ADP-ribosyltransferase C2 and C3 toxin-like 4
Short name:
ARTC4
Dombrock blood group carrier molecule
Mono(ADP-ribosyl)transferase 4
NAD(P)(+)--arginine ADP-ribosyltransferase 4
CD_antigen: CD297
Gene namesi
Name:ART4
Synonyms:DO, DOK1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000111339.10
HGNCiHGNC:726 ART4
MIMi110600 gene
neXtProtiNX_Q93070

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

MalaCardsiART4
MIMi616060 phenotype
PharmGKBiPA142672580

Polymorphism and mutation databases

BioMutaiART4
DMDMi22261809

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 46Sequence analysisAdd BLAST46
ChainiPRO_000001932947 – 285Ecto-ADP-ribosyltransferase 4Add BLAST239
PropeptideiPRO_0000019330286 – 314Removed in mature formSequence analysisAdd BLAST29

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi69 ↔ 280By similarity
Glycosylationi114N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi178N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi182 ↔ 231By similarity
Glycosylationi222N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi257N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi274N-linked (GlcNAc...) asparagineSequence analysis1
Lipidationi285GPI-anchor amidated alanineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiQ93070
PeptideAtlasiQ93070
PRIDEiQ93070

PTM databases

iPTMnetiQ93070
PhosphoSitePlusiQ93070

Expressioni

Tissue specificityi

Expressed in spleen and T-cells.

Gene expression databases

BgeeiENSG00000111339
CleanExiHS_ART4
HS_DOK1
ExpressionAtlasiQ93070 baseline and differential
GenevisibleiQ93070 HS

Organism-specific databases

HPAiHPA075664

Interactioni

Protein-protein interaction databases

BioGridi106913, 9 interactors
STRINGi9606.ENSP00000228936

Structurei

3D structure databases

ProteinModelPortaliQ93070
SMRiQ93070
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IFER Eukaryota
ENOG4111WZG LUCA
HOGENOMiHOG000273888
HOVERGENiHBG004464
InParanoidiQ93070
KOiK06717
OrthoDBiEOG091G0BSN
PhylomeDBiQ93070
TreeFamiTF335356

Family and domain databases

InterProiView protein in InterPro
IPR000768 ART
PfamiView protein in Pfam
PF01129 ART, 1 hit
PRINTSiPR00970 RIBTRNSFRASE
PROSITEiView protein in PROSITE
PS01291 ART, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q93070-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGPLINRCKK ILLPTTVPPA TMRIWLLGGL LPFLLLLSGL QRPTEGSEVA
60 70 80 90 100
IKIDFDFAPG SFDDQYQGCS KQVMEKLTQG DYFTKDIEAQ KNYFRMWQKA
110 120 130 140 150
HLAWLNQGKV LPQNMTTTHA VAILFYTLNS NVHSDFTRAM ASVARTPQQY
160 170 180 190 200
ERSFHFKYLH YYLTSAIQLL RKDSIMENGT LCYEVHYRTK DVHFNAYTGA
210 220 230 240 250
TIRFGQFLST SLLKEEAQEF GNQTLFTIFT CLGAPVQYFS LKKEVLIPPY
260 270 280 290 300
ELFKVINMSY HPRGNWLQLR STGNLSTYNC QLLKASSKKC IPDPIAIASL
310
SFLTSVIIFS KSRV
Length:314
Mass (Da):35,878
Last modified:August 13, 2002 - v2
Checksum:i174913890C9D158F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti48E → Q in CAA65095 (PubMed:9119374).Curated1
Sequence conflicti116T → S in CAA65095 (PubMed:9119374).Curated1

Polymorphismi

DO is responsible for the Dombrock blood group system [MIMi:616060]. The molecular basis of the Do(a)/Do(b) blood group antigen is a single variation in position 265; Asn-265 corresponds to Do(a) and Asp-265 to Do(b). It is also responsible for the antigens Gregory [Gy(a)], Holley [Hy] and Joseph [Jo(a)].2 Publications

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_013707108G → V in Hy1 and Hy2. 1 PublicationCorresponds to variant dbSNP:rs28362797Ensembl.1
Natural variantiVAR_013708117T → I in Jo(a). 1 PublicationCorresponds to variant dbSNP:rs28362798Ensembl.1
Natural variantiVAR_022266135D → E1 PublicationCorresponds to variant dbSNP:rs28362799Ensembl.1
Natural variantiVAR_022267189T → M1 PublicationCorresponds to variant dbSNP:rs28362800Ensembl.1
Natural variantiVAR_013709265N → D in Do(b), Hy1 and Hy2. 1 PublicationCorresponds to variant dbSNP:rs11276Ensembl.1
Natural variantiVAR_013710300L → V in Hy1. 1 PublicationCorresponds to variant dbSNP:rs3088190Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF290204 mRNA Translation: AAG17845.1
AF382213, AF382211, AF382212 Genomic DNA Translation: AAM21462.1
AF382216, AF382214, AF382215 Genomic DNA Translation: AAM21464.1
AF382219, AF382217, AF382218 Genomic DNA Translation: AAM21465.1
AF382222, AF382220, AF382221 Genomic DNA Translation: AAM21466.1
AF382225, AF382223, AF382224 Genomic DNA Translation: AAM21467.1
AY899803 Genomic DNA Translation: AAW65375.1
BC074727 mRNA Translation: AAH74727.1
X95826 Genomic DNA Translation: CAA65095.1
AF340233 Genomic DNA Translation: AAK11274.1
AF340234 Genomic DNA Translation: AAK11275.1
CCDSiCCDS8668.1
RefSeqiNP_066549.2, NM_021071.2
UniGeneiHs.591158
Hs.655792
Hs.668803

Genome annotation databases

EnsembliENST00000228936; ENSP00000228936; ENSG00000111339
GeneIDi420
KEGGihsa:420
UCSCiuc001rcl.2 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiNAR4_HUMAN
AccessioniPrimary (citable) accession number: Q93070
Secondary accession number(s): Q9BZ50, Q9BZ51, Q9HB06
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: August 13, 2002
Last modified: May 23, 2018
This is version 151 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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