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Protein

Ecto-ADP-ribosyltransferase 4

Gene

ART4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

NAD+ + protein-L-arginine = nicotinamide + N(omega)-(ADP-D-ribosyl)-protein-L-arginine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei126 – 1261NADBy similarity
Binding sitei206 – 2061NADBy similarity
Binding sitei240 – 2401NADBy similarity
Active sitei251 – 2511By similarity

GO - Molecular functioni

  • NAD(P)+-protein-arginine ADP-ribosyltransferase activity Source: UniProtKB

GO - Biological processi

  • arginine metabolic process Source: UniProtKB
  • protein ADP-ribosylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Blood group antigen, Glycosyltransferase, Transferase

Keywords - Ligandi

NAD, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Ecto-ADP-ribosyltransferase 4 (EC:2.4.2.31)
Alternative name(s):
ADP-ribosyltransferase C2 and C3 toxin-like 4
Short name:
ARTC4
Dombrock blood group carrier molecule
Mono(ADP-ribosyl)transferase 4
NAD(P)(+)--arginine ADP-ribosyltransferase 4
CD_antigen: CD297
Gene namesi
Name:ART4
Synonyms:DO, DOK1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:726. ART4.

Subcellular locationi

GO - Cellular componenti

  • anchored component of membrane Source: UniProtKB-KW
  • membrane Source: UniProtKB
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

MIMi616060. phenotype.
PharmGKBiPA142672580.

Polymorphism and mutation databases

BioMutaiART4.
DMDMi22261809.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4646Sequence AnalysisAdd
BLAST
Chaini47 – 285239Ecto-ADP-ribosyltransferase 4PRO_0000019329Add
BLAST
Propeptidei286 – 31429Removed in mature formSequence AnalysisPRO_0000019330Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi69 ↔ 280By similarity
Glycosylationi114 – 1141N-linked (GlcNAc...)Sequence Analysis
Glycosylationi178 – 1781N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi182 ↔ 231By similarity
Glycosylationi222 – 2221N-linked (GlcNAc...)Sequence Analysis
Glycosylationi257 – 2571N-linked (GlcNAc...)Sequence Analysis
Glycosylationi274 – 2741N-linked (GlcNAc...)Sequence Analysis
Lipidationi285 – 2851GPI-anchor amidated alanineSequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiQ93070.
PRIDEiQ93070.

PTM databases

PhosphoSiteiQ93070.

Expressioni

Tissue specificityi

Expressed in spleen and T-cells.

Gene expression databases

BgeeiQ93070.
CleanExiHS_ART4.
HS_DOK1.
ExpressionAtlasiQ93070. baseline and differential.
GenevisibleiQ93070. HS.

Organism-specific databases

HPAiHPA040879.

Interactioni

Protein-protein interaction databases

BioGridi106913. 2 interactions.
STRINGi9606.ENSP00000228936.

Structurei

3D structure databases

ProteinModelPortaliQ93070.
SMRiQ93070. Positions 55-256.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG44842.
HOGENOMiHOG000273888.
HOVERGENiHBG004464.
InParanoidiQ93070.
KOiK06717.
OrthoDBiEOG7D85WV.
PhylomeDBiQ93070.
TreeFamiTF335356.

Family and domain databases

InterProiIPR000768. ART.
[Graphical view]
PANTHERiPTHR10339. PTHR10339. 1 hit.
PfamiPF01129. ART. 1 hit.
[Graphical view]
PRINTSiPR00970. RIBTRNSFRASE.
PROSITEiPS01291. ART. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q93070-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGPLINRCKK ILLPTTVPPA TMRIWLLGGL LPFLLLLSGL QRPTEGSEVA
60 70 80 90 100
IKIDFDFAPG SFDDQYQGCS KQVMEKLTQG DYFTKDIEAQ KNYFRMWQKA
110 120 130 140 150
HLAWLNQGKV LPQNMTTTHA VAILFYTLNS NVHSDFTRAM ASVARTPQQY
160 170 180 190 200
ERSFHFKYLH YYLTSAIQLL RKDSIMENGT LCYEVHYRTK DVHFNAYTGA
210 220 230 240 250
TIRFGQFLST SLLKEEAQEF GNQTLFTIFT CLGAPVQYFS LKKEVLIPPY
260 270 280 290 300
ELFKVINMSY HPRGNWLQLR STGNLSTYNC QLLKASSKKC IPDPIAIASL
310
SFLTSVIIFS KSRV
Length:314
Mass (Da):35,878
Last modified:August 13, 2002 - v2
Checksum:i174913890C9D158F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti48 – 481E → Q in CAA65095 (PubMed:9119374).Curated
Sequence conflicti116 – 1161T → S in CAA65095 (PubMed:9119374).Curated

Polymorphismi

DO is responsible for the Dombrock blood group system [MIMi:616060]. The molecular basis of the Do(a)/Do(b) blood group antigen is a single variation in position 265; Asn-265 corresponds to Do(a) and Asp-265 to Do(b). It is also responsible for the antigens Gregory [Gy(a)], Holley [Hy] and Joseph [Jo(a)].2 Publications

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti108 – 1081G → V in Hy1 and Hy2. 1 Publication
Corresponds to variant rs28362797 [ dbSNP | Ensembl ].
VAR_013707
Natural varianti117 – 1171T → I in Jo(a). 1 Publication
Corresponds to variant rs28362798 [ dbSNP | Ensembl ].
VAR_013708
Natural varianti135 – 1351D → E.1 Publication
Corresponds to variant rs28362799 [ dbSNP | Ensembl ].
VAR_022266
Natural varianti189 – 1891T → M.1 Publication
Corresponds to variant rs28362800 [ dbSNP | Ensembl ].
VAR_022267
Natural varianti265 – 2651N → D in Do(b), Hy1 and Hy2. 1 Publication
Corresponds to variant rs11276 [ dbSNP | Ensembl ].
VAR_013709
Natural varianti300 – 3001L → V in Hy1. 1 Publication
Corresponds to variant rs3088190 [ dbSNP | Ensembl ].
VAR_013710

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF290204 mRNA. Translation: AAG17845.1.
AF382213, AF382211, AF382212 Genomic DNA. Translation: AAM21462.1.
AF382216, AF382214, AF382215 Genomic DNA. Translation: AAM21464.1.
AF382219, AF382217, AF382218 Genomic DNA. Translation: AAM21465.1.
AF382222, AF382220, AF382221 Genomic DNA. Translation: AAM21466.1.
AF382225, AF382223, AF382224 Genomic DNA. Translation: AAM21467.1.
AY899803 Genomic DNA. Translation: AAW65375.1.
BC074727 mRNA. Translation: AAH74727.1.
X95826 Genomic DNA. Translation: CAA65095.1.
AF340233 Genomic DNA. Translation: AAK11274.1.
AF340234 Genomic DNA. Translation: AAK11275.1.
CCDSiCCDS8668.1.
RefSeqiNP_066549.2. NM_021071.2.
UniGeneiHs.591158.
Hs.655792.
Hs.668803.

Genome annotation databases

EnsembliENST00000228936; ENSP00000228936; ENSG00000111339.
GeneIDi420.
KEGGihsa:420.
UCSCiuc001rcl.1. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

dbRBC/BGMUT

Blood group antigen gene mutation database

SeattleSNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF290204 mRNA. Translation: AAG17845.1.
AF382213, AF382211, AF382212 Genomic DNA. Translation: AAM21462.1.
AF382216, AF382214, AF382215 Genomic DNA. Translation: AAM21464.1.
AF382219, AF382217, AF382218 Genomic DNA. Translation: AAM21465.1.
AF382222, AF382220, AF382221 Genomic DNA. Translation: AAM21466.1.
AF382225, AF382223, AF382224 Genomic DNA. Translation: AAM21467.1.
AY899803 Genomic DNA. Translation: AAW65375.1.
BC074727 mRNA. Translation: AAH74727.1.
X95826 Genomic DNA. Translation: CAA65095.1.
AF340233 Genomic DNA. Translation: AAK11274.1.
AF340234 Genomic DNA. Translation: AAK11275.1.
CCDSiCCDS8668.1.
RefSeqiNP_066549.2. NM_021071.2.
UniGeneiHs.591158.
Hs.655792.
Hs.668803.

3D structure databases

ProteinModelPortaliQ93070.
SMRiQ93070. Positions 55-256.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106913. 2 interactions.
STRINGi9606.ENSP00000228936.

PTM databases

PhosphoSiteiQ93070.

Polymorphism and mutation databases

BioMutaiART4.
DMDMi22261809.

Proteomic databases

PaxDbiQ93070.
PRIDEiQ93070.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000228936; ENSP00000228936; ENSG00000111339.
GeneIDi420.
KEGGihsa:420.
UCSCiuc001rcl.1. human.

Organism-specific databases

CTDi420.
GeneCardsiGC12M014982.
H-InvDBHIX0036719.
HGNCiHGNC:726. ART4.
HPAiHPA040879.
MIMi110600. gene.
616060. phenotype.
neXtProtiNX_Q93070.
PharmGKBiPA142672580.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG44842.
HOGENOMiHOG000273888.
HOVERGENiHBG004464.
InParanoidiQ93070.
KOiK06717.
OrthoDBiEOG7D85WV.
PhylomeDBiQ93070.
TreeFamiTF335356.

Miscellaneous databases

GeneWikiiART4.
GenomeRNAii420.
NextBioi1775.
PROiQ93070.
SOURCEiSearch...

Gene expression databases

BgeeiQ93070.
CleanExiHS_ART4.
HS_DOK1.
ExpressionAtlasiQ93070. baseline and differential.
GenevisibleiQ93070. HS.

Family and domain databases

InterProiIPR000768. ART.
[Graphical view]
PANTHERiPTHR10339. PTHR10339. 1 hit.
PfamiPF01129. ART. 1 hit.
[Graphical view]
PRINTSiPR00970. RIBTRNSFRASE.
PROSITEiPS01291. ART. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of the Dombrock blood group glycoprotein as a polymorphic member of the ADP-ribosyltransferase gene family."
    Gubin A.N., Njoroge J.M., Wojda U., Pack S.D., Rios M., Reid M.E., Miller J.L.
    Blood 96:2621-2627(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], POLYMORPHISM, VARIANT ASP-265.
  2. "Insights into the Holley- and Joseph- phenotypes."
    Rios M., Hue-Roye K., Oyen R., Miller J., Reid M.E.
    Transfusion 42:52-58(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], POLYMORPHISM, VARIANTS VAL-108; ILE-117; ASP-265 AND VAL-300.
  3. SeattleSNPs variation discovery resource
    Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS VAL-108; ILE-117; GLU-135; MET-189; ASP-265 AND VAL-300.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  5. "Two novel human members of an emerging mammalian gene family related to mono-ADP-ribosylating bacterial toxins."
    Koch-Nolte F., Haag F., Braren R., Kuehl M., Hoovers J., Balasubramanian S., Bazan J.F., Thiele H.-G.
    Genomics 39:370-376(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 48-314.
  6. "Polymerase chain reaction with sequence-specific primers-based genotyping of the human Dombrock blood group DO1 and DO2 alleles and the DO gene frequencies in Chinese blood donors."
    Wu G.-G., Jin S.-Z., Deng Z.-H., Zhao T.-M.
    Vox Sang. 81:49-51(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 49-284, POLYMORPHISM, VARIANT ASP-265.
  7. "Toward a unified nomenclature for mammalian ADP-ribosyltransferases."
    Hottiger M.O., Hassa P.O., Luscher B., Schuler H., Koch-Nolte F.
    Trends Biochem. Sci. 35:208-219(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE.

Entry informationi

Entry nameiNAR4_HUMAN
AccessioniPrimary (citable) accession number: Q93070
Secondary accession number(s): Q9BZ50, Q9BZ51, Q9HB06
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: August 13, 2002
Last modified: June 24, 2015
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Blood group antigen proteins
    Nomenclature of blood group antigens and list of entries
  2. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
  3. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  4. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  5. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  6. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  7. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.