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Protein

Ecto-ADP-ribosyltransferase 4

Gene

ART4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

NAD+ + protein-L-arginine = nicotinamide + N(omega)-(ADP-D-ribosyl)-protein-L-arginine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei126NADBy similarity1
Binding sitei206NADBy similarity1
Binding sitei240NADBy similarity1
Active sitei251By similarity1

GO - Molecular functioni

  • NAD(P)+-protein-arginine ADP-ribosyltransferase activity Source: UniProtKB

GO - Biological processi

  • arginine metabolic process Source: UniProtKB
  • C-terminal protein lipidation Source: Reactome
  • protein ADP-ribosylation Source: UniProtKB

Keywordsi

Molecular functionBlood group antigen, Glycosyltransferase, Transferase
LigandNAD, NADP

Enzyme and pathway databases

ReactomeiR-HSA-163125. Post-translational modification: synthesis of GPI-anchored proteins.

Names & Taxonomyi

Protein namesi
Recommended name:
Ecto-ADP-ribosyltransferase 4 (EC:2.4.2.31)
Alternative name(s):
ADP-ribosyltransferase C2 and C3 toxin-like 4
Short name:
ARTC4
Dombrock blood group carrier molecule
Mono(ADP-ribosyl)transferase 4
NAD(P)(+)--arginine ADP-ribosyltransferase 4
CD_antigen: CD297
Gene namesi
Name:ART4
Synonyms:DO, DOK1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000111339.10.
HGNCiHGNC:726. ART4.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

MIMi616060. phenotype.
PharmGKBiPA142672580.

Polymorphism and mutation databases

BioMutaiART4.
DMDMi22261809.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 46Sequence analysisAdd BLAST46
ChainiPRO_000001932947 – 285Ecto-ADP-ribosyltransferase 4Add BLAST239
PropeptideiPRO_0000019330286 – 314Removed in mature formSequence analysisAdd BLAST29

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi69 ↔ 280By similarity
Glycosylationi114N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi178N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi182 ↔ 231By similarity
Glycosylationi222N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi257N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi274N-linked (GlcNAc...) asparagineSequence analysis1
Lipidationi285GPI-anchor amidated alanineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiQ93070.
PeptideAtlasiQ93070.
PRIDEiQ93070.

PTM databases

iPTMnetiQ93070.
PhosphoSitePlusiQ93070.

Expressioni

Tissue specificityi

Expressed in spleen and T-cells.

Gene expression databases

BgeeiENSG00000111339.
CleanExiHS_ART4.
HS_DOK1.
ExpressionAtlasiQ93070. baseline and differential.
GenevisibleiQ93070. HS.

Organism-specific databases

HPAiHPA075664.

Interactioni

Protein-protein interaction databases

BioGridi106913. 9 interactors.
STRINGi9606.ENSP00000228936.

Structurei

3D structure databases

ProteinModelPortaliQ93070.
SMRiQ93070.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IFER. Eukaryota.
ENOG4111WZG. LUCA.
HOGENOMiHOG000273888.
HOVERGENiHBG004464.
InParanoidiQ93070.
KOiK06717.
OrthoDBiEOG091G0BSN.
PhylomeDBiQ93070.
TreeFamiTF335356.

Family and domain databases

InterProiView protein in InterPro
IPR000768. ART.
PfamiView protein in Pfam
PF01129. ART. 1 hit.
PRINTSiPR00970. RIBTRNSFRASE.
PROSITEiView protein in PROSITE
PS01291. ART. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q93070-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGPLINRCKK ILLPTTVPPA TMRIWLLGGL LPFLLLLSGL QRPTEGSEVA
60 70 80 90 100
IKIDFDFAPG SFDDQYQGCS KQVMEKLTQG DYFTKDIEAQ KNYFRMWQKA
110 120 130 140 150
HLAWLNQGKV LPQNMTTTHA VAILFYTLNS NVHSDFTRAM ASVARTPQQY
160 170 180 190 200
ERSFHFKYLH YYLTSAIQLL RKDSIMENGT LCYEVHYRTK DVHFNAYTGA
210 220 230 240 250
TIRFGQFLST SLLKEEAQEF GNQTLFTIFT CLGAPVQYFS LKKEVLIPPY
260 270 280 290 300
ELFKVINMSY HPRGNWLQLR STGNLSTYNC QLLKASSKKC IPDPIAIASL
310
SFLTSVIIFS KSRV
Length:314
Mass (Da):35,878
Last modified:August 13, 2002 - v2
Checksum:i174913890C9D158F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti48E → Q in CAA65095 (PubMed:9119374).Curated1
Sequence conflicti116T → S in CAA65095 (PubMed:9119374).Curated1

Polymorphismi

DO is responsible for the Dombrock blood group system [MIMi:616060]. The molecular basis of the Do(a)/Do(b) blood group antigen is a single variation in position 265; Asn-265 corresponds to Do(a) and Asp-265 to Do(b). It is also responsible for the antigens Gregory [Gy(a)], Holley [Hy] and Joseph [Jo(a)].2 Publications

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_013707108G → V in Hy1 and Hy2. 1 PublicationCorresponds to variant dbSNP:rs28362797Ensembl.1
Natural variantiVAR_013708117T → I in Jo(a). 1 PublicationCorresponds to variant dbSNP:rs28362798Ensembl.1
Natural variantiVAR_022266135D → E1 PublicationCorresponds to variant dbSNP:rs28362799Ensembl.1
Natural variantiVAR_022267189T → M1 PublicationCorresponds to variant dbSNP:rs28362800Ensembl.1
Natural variantiVAR_013709265N → D in Do(b), Hy1 and Hy2. 1 PublicationCorresponds to variant dbSNP:rs11276Ensembl.1
Natural variantiVAR_013710300L → V in Hy1. 1 PublicationCorresponds to variant dbSNP:rs3088190Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF290204 mRNA. Translation: AAG17845.1.
AF382213, AF382211, AF382212 Genomic DNA. Translation: AAM21462.1.
AF382216, AF382214, AF382215 Genomic DNA. Translation: AAM21464.1.
AF382219, AF382217, AF382218 Genomic DNA. Translation: AAM21465.1.
AF382222, AF382220, AF382221 Genomic DNA. Translation: AAM21466.1.
AF382225, AF382223, AF382224 Genomic DNA. Translation: AAM21467.1.
AY899803 Genomic DNA. Translation: AAW65375.1.
BC074727 mRNA. Translation: AAH74727.1.
X95826 Genomic DNA. Translation: CAA65095.1.
AF340233 Genomic DNA. Translation: AAK11274.1.
AF340234 Genomic DNA. Translation: AAK11275.1.
CCDSiCCDS8668.1.
RefSeqiNP_066549.2. NM_021071.2.
UniGeneiHs.591158.
Hs.655792.
Hs.668803.

Genome annotation databases

EnsembliENST00000228936; ENSP00000228936; ENSG00000111339.
GeneIDi420.
KEGGihsa:420.
UCSCiuc001rcl.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiNAR4_HUMAN
AccessioniPrimary (citable) accession number: Q93070
Secondary accession number(s): Q9BZ50, Q9BZ51, Q9HB06
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: August 13, 2002
Last modified: September 27, 2017
This is version 147 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Blood group antigen proteins
    Nomenclature of blood group antigens and list of entries
  2. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
  3. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  4. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  5. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  6. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  7. SIMILARITY comments
    Index of protein domains and families