RNA-binding protein with multiple splicing

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Q93062 (RBPMS_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 123. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
RNA-binding protein with multiple splicing
Short name=RBP-MS

Alternative name(s):
Heart and RRM expressed sequence
Short name=Hermes

Gene names

Name:	RBPMS
Synonyms:	HERMES

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	196 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a coactivator of transcriptional activity. Required to increase TGFB1/Smad-mediated transactivation. Acts through SMAD2, SMAD3 and SMAD4 to increase transcriptional activity. Increases phosphorylation of SMAD2 and SMAD3 on their C-terminal SSXS motif, possibly through recruitment of TGFBR1. Promotes the nuclear accumulation of SMAD2, SMAD3 and SMAD4 proteins. Binds to poly(A) RNA. Ref.5
Subunit structure	Interacts with SMAD2, SMAD3 and SMAD4; the interactions are direct. Ref.5
Subcellular location	Nucleus. Cytoplasm Ref.5.
Tissue specificity	Ubiquitously expressed, at various levels depending on the isoform and the tissue.
Domain	The RRM domain is necessary for interaction with SMAD4. Both the RRM domain and the C-terminus are required for TGFB1/Smad-mediated transactivation activity.
Sequence similarities	Contains 1 RRM (RNA recognition motif) domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Ligand	RNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA processing Traceable author statement Ref.1. Source: ProtInc positive regulation of SMAD protein import into nucleus Inferred from direct assay Ref.5. Source: UniProtKB positive regulation of pathway-restricted SMAD protein phosphorylation Inferred from direct assay Ref.5. Source: UniProtKB regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	nucleotide binding Inferred from electronic annotation. Source: InterPro poly(A) RNA binding Inferred from direct assay Ref.5. Source: UniProtKB transcription coactivator activity Inferred from direct assay Ref.5. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ATXN1	P54253	3	EBI-740322,EBI-930964
Hoxa1	P09022	3	EBI-740322,EBI-3957603	From a different organism.
QKI	Q96PU8	2	EBI-740322,EBI-945792
SMUG1	Q53HV7	2	EBI-740322,EBI-749970

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select] Note: Additional isoforms seem to exist.

Isoform A (identifier: Q93062-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Isoform B (identifier: Q93062-2) The sequence of this isoform differs from the canonical sequence as follows: 177-196: MRWLPPSEATSQGWKSRQFC → LCEGQTVRRSHPLSAPSPDSASLAWFPV

Isoform C (identifier: Q93062-3) The sequence of this isoform differs from the canonical sequence as follows: 177-196: MRWLPPSEATSQGWKSRQFC → CFSPEAKPNTPVFCPLLQQIRFVSGNVFVTYQPTADQQRELPC

Isoform D (identifier: Q93062-4) The sequence of this isoform differs from the canonical sequence as follows: 133-196: YELTVPALYPSSPEVWAPYPLYPAELAPALPPPAFTYPASLHAQMRWLPPSEATSQGWKSRQFC → LGGANEHGERQ

Isoform E (identifier: Q93062-5) The sequence of this isoform differs from the canonical sequence as follows: 133-176: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 196

196

RNA-binding protein with multiple splicing

PRO_0000081793

Regions

Domain

24 – 101

RRM

Amino acid modifications

Modified residue

113

Phosphothreonine Ref.6

Natural variations

Alternative sequence

133 – 196

YELTV…SRQFC → LGGANEHGERQ in isoform D.

VSP_005815

Alternative sequence

133 – 176

Missing in isoform E.

VSP_005816

Alternative sequence

177 – 196

MRWLP…SRQFC → LCEGQTVRRSHPLSAPSPDS ASLAWFPV in isoform B.

VSP_005813

Alternative sequence

177 – 196

MRWLP…SRQFC → CFSPEAKPNTPVFCPLLQQI RFVSGNVFVTYQPTADQQRE LPC in isoform C.

VSP_005814

Secondary structure

196

Helix Strand Turn

Details...

Sequences

Sequence		Length	Mass (Da)
Isoform A [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 05DD964E62F8F88C Show »	FASTA	196	21,802
10 20 30 40 50 60 MNNGGKAEKE NTPSEANLQE EEVRTLFVSG LPLDIKPREL YLLFRPFKGY EGSLIKLTSK 70 80 90 100 110 120 QPVGFVSFDS RSEAEAAKNA LNGIRFDPEI PQTLRLEFAK ANTKMAKNKL VGTPNPSTPL 130 140 150 160 170 180 PNTVPQFIAR EPYELTVPAL YPSSPEVWAP YPLYPAELAP ALPPPAFTYP ASLHAQMRWL 190 PPSEATSQGW KSRQFC « Hide
Isoform B [UniParc]. Checksum: 60382A7EC1D4672F Show »	FASTA	204	22,416
10 20 30 40 50 60 MNNGGKAEKE NTPSEANLQE EEVRTLFVSG LPLDIKPREL YLLFRPFKGY EGSLIKLTSK 70 80 90 100 110 120 QPVGFVSFDS RSEAEAAKNA LNGIRFDPEI PQTLRLEFAK ANTKMAKNKL VGTPNPSTPL 130 140 150 160 170 180 PNTVPQFIAR EPYELTVPAL YPSSPEVWAP YPLYPAELAP ALPPPAFTYP ASLHAQLCEG 190 200 QTVRRSHPLS APSPDSASLA WFPV « Hide
Isoform C [UniParc]. Checksum: 700E9B9FA7682FAD Show »	FASTA	219	24,277
10 20 30 40 50 60 MNNGGKAEKE NTPSEANLQE EEVRTLFVSG LPLDIKPREL YLLFRPFKGY EGSLIKLTSK 70 80 90 100 110 120 QPVGFVSFDS RSEAEAAKNA LNGIRFDPEI PQTLRLEFAK ANTKMAKNKL VGTPNPSTPL 130 140 150 160 170 180 PNTVPQFIAR EPYELTVPAL YPSSPEVWAP YPLYPAELAP ALPPPAFTYP ASLHAQCFSP 190 200 210 EAKPNTPVFC PLLQQIRFVS GNVFVTYQPT ADQQRELPC « Hide
Isoform D [UniParc]. Checksum: 86DDB14345784716 Show »	FASTA	143	15,794
10 20 30 40 50 60 MNNGGKAEKE NTPSEANLQE EEVRTLFVSG LPLDIKPREL YLLFRPFKGY EGSLIKLTSK 70 80 90 100 110 120 QPVGFVSFDS RSEAEAAKNA LNGIRFDPEI PQTLRLEFAK ANTKMAKNKL VGTPNPSTPL 130 140 PNTVPQFIAR EPLGGANEHG ERQ « Hide
Isoform E [UniParc]. Checksum: B62DC25BB6EFB888 Show »	FASTA	152	17,022
10 20 30 40 50 60 MNNGGKAEKE NTPSEANLQE EEVRTLFVSG LPLDIKPREL YLLFRPFKGY EGSLIKLTSK 70 80 90 100 110 120 QPVGFVSFDS RSEAEAAKNA LNGIRFDPEI PQTLRLEFAK ANTKMAKNKL VGTPNPSTPL 130 140 150 PNTVPQFIAR EPMRWLPPSE ATSQGWKSRQ FC « Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A unique human gene that spans over 230 kb in the human chromosome 8p11-12 and codes multiple family proteins sharing RNA-binding motifs." Shimamoto A., Kitao S., Ichikawa K., Suzuki N., Yamabe Y., Imamura O., Tokutake Y., Satoh M., Matsumoto T., Kuromitsu J., Kataoka H., Sugawara K., Sugawara M., Sugimoto M., Goto M., Furuichi Y. Proc. Natl. Acad. Sci. U.S.A. 93:10913-10917(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A; B; C; D AND E). Tissue: Lip.
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM C). Tissue: Placenta.
[4]	"An unappreciated role for RNA surveillance." Hillman R.T., Green R.E., Brenner S.E. Genome Biol. 5:R8.1-R8.16(2004) [PubMed] [Europe PMC] [Abstract] Cited for: SPLICE ISOFORM(S) THAT ARE POTENTIAL NMD TARGET(S).
[5]	"Potentiation of Smad-mediated transcriptional activation by the RNA-binding protein RBPMS." Sun Y., Ding L., Zhang H., Han J., Yang X., Yan J., Zhu Y., Li J., Song H., Ye Q. Nucleic Acids Res. 34:6314-6326(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH SMAD2; SMAD3; SMAD4 AND TGFBR1, SUBCELLULAR LOCATION, RNA-BINDING.
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-113, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D84110 mRNA. Translation: BAA12228.1.
D84107 mRNA. Translation: BAA12225.1.
D84108 mRNA. Translation: BAA12226.1.
D84109 mRNA. Translation: BAA12227.1.
D84111 mRNA. Translation: BAA12229.1.
CH471080 Genomic DNA. Translation: EAW63451.1.
CH471080 Genomic DNA. Translation: EAW63453.1.
BC003608 mRNA. Translation: AAH03608.1.

IPI

IPI00004045.
IPI00101800.
IPI00216935.
IPI00216936.
IPI00414709.

PIR

JC6127.

RefSeq

NP_001008710.1. NM_001008710.1.
NP_001008711.1. NM_001008711.1.
NP_001008712.1. NM_001008712.1.
NP_006858.1. NM_006867.2.

UniGene

Hs.334587.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BNY	model	-	A	15-111	[»]

ProteinModelPortal

Q93062.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q93062. 56 interactions.

MINT

MINT-1434067.

STRING

9606.ENSP00000340176.

Polymorphism databases

DMDM

13124469.

Proteomic databases

PaxDb

Q93062.

PRIDE

Q93062.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000287771; ENSP00000287771; ENSG00000157110.
ENST00000320203; ENSP00000318102; ENSG00000157110.
ENST00000339877; ENSP00000340176; ENSG00000157110.
ENST00000397323; ENSP00000380486; ENSG00000157110.

GeneID

11030.

KEGG

hsa:11030.

UCSC

uc003xib.3. human.
uc003xic.1. human.
uc003xid.1. human.

Organism-specific databases

CTD

11030.

GeneCards

GC08P030242.

HGNC

HGNC:19097. RBPMS.

MIM

601558. gene.

neXtProt

NX_Q93062.

PharmGKB

PA134985584.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG292253.

HOGENOM

HOG000017369.

HOVERGEN

HBG069897.

OMA

PEVWGPY.

OrthoDB

EOG4NGGNV.

Gene expression databases

ArrayExpress

Q93062.

Bgee

Q93062.

CleanEx

HS_RBPMS.

Genevestigator

Q93062.

GermOnline

ENSG00000157110. Homo sapiens.

Family and domain databases

Gene3D

3.30.70.330. 1 hit.

InterPro

IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]

Pfam

PF00076. RRM_1. 1 hit.
[Graphical view]

SMART

SM00360. RRM. 1 hit.
[Graphical view]

PROSITE

PS50102. RRM. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

RBPMS. human.

GenomeRNAi

11030.

NextBio

41912.

SOURCE

Search...

Entry information

Entry name

RBPMS_HUMAN

Accession

Primary (citable) accession number: Q93062
Secondary accession number(s): D3DSU9

Q96J26

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 123 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 8

Human chromosome 8: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Polymorphism databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents