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Protein

V-type proton ATPase 116 kDa subunit a isoform 1

Gene

ATP6V0A1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for assembly and activity of the vacuolar ATPase. Potential role in differential targeting and regulation of the enzyme for a specific organelle (By similarity).By similarity

GO - Molecular functioni

  • ATPase binding Source: UniProtKB
  • proton-transporting ATPase activity, rotational mechanism Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Hydrogen ion transport, Ion transport, Transport

Enzyme and pathway databases

BioCyciMetaCyc:ENSG00000033627-MONOMER.
ZFISH:ENSG00000033627-MONOMER.
ReactomeiR-HSA-1222556. ROS, RNS production in response to bacteria.
R-HSA-6798695. Neutrophil degranulation.
R-HSA-77387. Insulin receptor recycling.
R-HSA-917977. Transferrin endocytosis and recycling.
R-HSA-983712. Ion channel transport.

Protein family/group databases

TCDBi3.A.2.2.4. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
V-type proton ATPase 116 kDa subunit a isoform 1
Short name:
V-ATPase 116 kDa isoform a1
Alternative name(s):
Clathrin-coated vesicle/synaptic vesicle proton pump 116 kDa subunit
Vacuolar adenosine triphosphatase subunit Ac116
Vacuolar proton pump subunit 1
Vacuolar proton translocating ATPase 116 kDa subunit a isoform 1
Gene namesi
Name:ATP6V0A1
Synonyms:ATP6N1, ATP6N1A, VPP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:865. ATP6V0A1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 388CytoplasmicSequence analysisAdd BLAST388
Transmembranei389 – 407HelicalSequence analysisAdd BLAST19
Topological domaini408 – 409VacuolarSequence analysis2
Transmembranei410 – 426HelicalSequence analysisAdd BLAST17
Topological domaini427 – 441CytoplasmicSequence analysisAdd BLAST15
Transmembranei442 – 471HelicalSequence analysisAdd BLAST30
Topological domaini472 – 534VacuolarSequence analysisAdd BLAST63
Transmembranei535 – 554HelicalSequence analysisAdd BLAST20
Topological domaini555 – 572CytoplasmicSequence analysisAdd BLAST18
Transmembranei573 – 593HelicalSequence analysisAdd BLAST21
Topological domaini594 – 638VacuolarSequence analysisAdd BLAST45
Transmembranei639 – 658HelicalSequence analysisAdd BLAST20
Topological domaini659 – 724CytoplasmicSequence analysisAdd BLAST66
Transmembranei725 – 749HelicalSequence analysisAdd BLAST25
Topological domaini750 – 770VacuolarSequence analysisAdd BLAST21
Transmembranei771 – 809HelicalSequence analysisAdd BLAST39
Topological domaini810 – 837CytoplasmicSequence analysisAdd BLAST28

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi535.
OpenTargetsiENSG00000033627.
PharmGKBiPA25146.

Polymorphism and mutation databases

BioMutaiATP6V0A1.
DMDMi59803038.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001192111 – 837V-type proton ATPase 116 kDa subunit a isoform 1Add BLAST837

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei250PhosphothreonineBy similarity1
Modified residuei360PhosphothreonineCombined sources1
Modified residuei364PhosphotyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ93050.
PeptideAtlasiQ93050.
PRIDEiQ93050.

PTM databases

iPTMnetiQ93050.
PhosphoSitePlusiQ93050.
SwissPalmiQ93050.

Expressioni

Gene expression databases

BgeeiENSG00000033627.
CleanExiHS_ATP6V0A1.
ExpressionAtlasiQ93050. baseline and differential.
GenevisibleiQ93050. HS.

Organism-specific databases

HPAiHPA022144.

Interactioni

Subunit structurei

The V-ATPase is a heteromultimeric enzyme composed of at least thirteen different subunits. It has a membrane peripheral V1 sector for ATP hydrolysis and an integral V0 for proton translocation. The V1 sector comprises subunits A-H, whereas V0 includes subunits a, d, c, c', and c''.

GO - Molecular functioni

  • ATPase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi107018. 77 interactors.
IntActiQ93050. 28 interactors.
MINTiMINT-3049850.
STRINGi9606.ENSP00000264649.

Structurei

3D structure databases

ProteinModelPortaliQ93050.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the V-ATPase 116 kDa subunit family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2189. Eukaryota.
COG1269. LUCA.
GeneTreeiENSGT00390000004941.
HOGENOMiHOG000037059.
HOVERGENiHBG014606.
InParanoidiQ93050.
KOiK02154.
OMAiFTLYNWT.
OrthoDBiEOG091G01BI.
PhylomeDBiQ93050.
TreeFamiTF300346.

Family and domain databases

InterProiIPR002490. V-ATPase_116kDa_su.
IPR026028. V-type_ATPase_116kDa_su_euka.
[Graphical view]
PANTHERiPTHR11629. PTHR11629. 1 hit.
PfamiPF01496. V_ATPase_I. 1 hit.
[Graphical view]
PIRSFiPIRSF001293. ATP6V0A1. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q93050-2) [UniParc]FASTAAdd to basket
Also known as: I

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGELFRSEEM TLAQLFLQSE AAYCCVSELG ELGKVQFRDL NPDVNVFQRK
60 70 80 90 100
FVNEVRRCEE MDRKLRFVEK EIRKANIPIM DTGENPEVPF PRDMIDLEAN
110 120 130 140 150
FEKIENELKE INTNQEALKR NFLELTELKF ILRKTQQFFD EMADPDLLEE
160 170 180 190 200
SSSLLEPSEM GRGTPLRLGF VAGVINRERI PTFERMLWRV CRGNVFLRQA
210 220 230 240 250
EIENPLEDPV TGDYVHKSVF IIFFQGDQLK NRVKKICEGF RASLYPCPET
260 270 280 290 300
PQERKEMASG VNTRIDDLQM VLNQTEDHRQ RVLQAAAKNI RVWFIKVRKM
310 320 330 340 350
KAIYHTLNLC NIDVTQKCLI AEVWCPVTDL DSIQFALRRG TEHSGSTVPS
360 370 380 390 400
ILNRMQTNQT PPTYNKTNKF TYGFQNIVDA YGIGTYREIN PAPYTIITFP
410 420 430 440 450
FLFAVMFGDF GHGILMTLFA VWMVLRESRI LSQKNENEMF STVFSGRYII
460 470 480 490 500
LLMGVFSMYT GLIYNDCFSK SLNIFGSSWS VRPMFTYNWT EETLRGNPVL
510 520 530 540 550
QLNPALPGVF GGPYPFGIDP IWNIATNKLT FLNSFKMKMS VILGIIHMLF
560 570 580 590 600
GVSLSLFNHI YFKKPLNIYF GFIPEIIFMT SLFGYLVILI FYKWTAYDAH
610 620 630 640 650
TSENAPSLLI HFINMFLFSY PESGYSMLYS GQKGIQCFLV VVALLCVPWM
660 670 680 690 700
LLFKPLVLRR QYLRRKHLGT LNFGGIRVGN GPTEEDAEII QHDQLSTHSE
710 720 730 740 750
DADEPSEDEV FDFGDTMVHQ AIHTIEYCLG CISNTASYLR LWALSLAHAQ
760 770 780 790 800
LSEVLWTMVI HIGLSVKSLA GGLVLFFFFT AFATLTVAIL LIMEGLSAFL
810 820 830
HALRLHWVEF QNKFYSGTGF KFLPFSFEHI REGKFEE
Length:837
Mass (Da):96,413
Last modified:February 15, 2005 - v3
Checksum:i80E4842CF6ADEE44
GO
Isoform 2 (identifier: Q93050-1) [UniParc]FASTAAdd to basket
Also known as: II

The sequence of this isoform differs from the canonical sequence as follows:
     705-710: Missing.

Show »
Length:831
Mass (Da):95,756
Checksum:iB16170B568A3D586
GO
Isoform 3 (identifier: Q93050-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     141-141: E → EAELHHQQ
     705-710: Missing.

Note: No experimental confirmation available.
Show »
Length:838
Mass (Da):96,600
Checksum:iBE9A648B592B7C67
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti750 – 751QL → HV in CAA96077 (Ref. 1) Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_043532141E → EAELHHQQ in isoform 3. 1 Publication1
Alternative sequenceiVSP_012814705 – 710Missing in isoform 2 and isoform 3. 3 Publications6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71460 mRNA. Translation: CAA96077.1.
AK295682 mRNA. Translation: BAH12153.1.
AK316305 mRNA. Translation: BAH14676.1.
AC067852 Genomic DNA. No translation available.
AC107993 Genomic DNA. No translation available.
CH471152 Genomic DNA. Translation: EAW60830.1.
BC032398 mRNA. Translation: AAH32398.1.
AL137683 mRNA. Translation: CAB70874.1.
CCDSiCCDS11426.1. [Q93050-1]
CCDS45683.1. [Q93050-3]
CCDS45684.1. [Q93050-2]
PIRiT46449.
RefSeqiNP_001123492.1. NM_001130020.1. [Q93050-3]
NP_001123493.1. NM_001130021.1. [Q93050-2]
NP_005168.2. NM_005177.3. [Q93050-1]
UniGeneiHs.463074.

Genome annotation databases

EnsembliENST00000264649; ENSP00000264649; ENSG00000033627. [Q93050-3]
ENST00000343619; ENSP00000342951; ENSG00000033627. [Q93050-2]
ENST00000393829; ENSP00000377415; ENSG00000033627. [Q93050-1]
GeneIDi535.
KEGGihsa:535.
UCSCiuc002hzq.4. human. [Q93050-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71460 mRNA. Translation: CAA96077.1.
AK295682 mRNA. Translation: BAH12153.1.
AK316305 mRNA. Translation: BAH14676.1.
AC067852 Genomic DNA. No translation available.
AC107993 Genomic DNA. No translation available.
CH471152 Genomic DNA. Translation: EAW60830.1.
BC032398 mRNA. Translation: AAH32398.1.
AL137683 mRNA. Translation: CAB70874.1.
CCDSiCCDS11426.1. [Q93050-1]
CCDS45683.1. [Q93050-3]
CCDS45684.1. [Q93050-2]
PIRiT46449.
RefSeqiNP_001123492.1. NM_001130020.1. [Q93050-3]
NP_001123493.1. NM_001130021.1. [Q93050-2]
NP_005168.2. NM_005177.3. [Q93050-1]
UniGeneiHs.463074.

3D structure databases

ProteinModelPortaliQ93050.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107018. 77 interactors.
IntActiQ93050. 28 interactors.
MINTiMINT-3049850.
STRINGi9606.ENSP00000264649.

Protein family/group databases

TCDBi3.A.2.2.4. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

PTM databases

iPTMnetiQ93050.
PhosphoSitePlusiQ93050.
SwissPalmiQ93050.

Polymorphism and mutation databases

BioMutaiATP6V0A1.
DMDMi59803038.

Proteomic databases

PaxDbiQ93050.
PeptideAtlasiQ93050.
PRIDEiQ93050.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000264649; ENSP00000264649; ENSG00000033627. [Q93050-3]
ENST00000343619; ENSP00000342951; ENSG00000033627. [Q93050-2]
ENST00000393829; ENSP00000377415; ENSG00000033627. [Q93050-1]
GeneIDi535.
KEGGihsa:535.
UCSCiuc002hzq.4. human. [Q93050-2]

Organism-specific databases

CTDi535.
DisGeNETi535.
GeneCardsiATP6V0A1.
H-InvDBHIX0013842.
HGNCiHGNC:865. ATP6V0A1.
HPAiHPA022144.
MIMi192130. gene.
neXtProtiNX_Q93050.
OpenTargetsiENSG00000033627.
PharmGKBiPA25146.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2189. Eukaryota.
COG1269. LUCA.
GeneTreeiENSGT00390000004941.
HOGENOMiHOG000037059.
HOVERGENiHBG014606.
InParanoidiQ93050.
KOiK02154.
OMAiFTLYNWT.
OrthoDBiEOG091G01BI.
PhylomeDBiQ93050.
TreeFamiTF300346.

Enzyme and pathway databases

BioCyciMetaCyc:ENSG00000033627-MONOMER.
ZFISH:ENSG00000033627-MONOMER.
ReactomeiR-HSA-1222556. ROS, RNS production in response to bacteria.
R-HSA-6798695. Neutrophil degranulation.
R-HSA-77387. Insulin receptor recycling.
R-HSA-917977. Transferrin endocytosis and recycling.
R-HSA-983712. Ion channel transport.

Miscellaneous databases

ChiTaRSiATP6V0A1. human.
GeneWikiiATPase,_H%2B_transporting,_lysosomal_V0_subunit_a1.
GenomeRNAii535.
PROiQ93050.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000033627.
CleanExiHS_ATP6V0A1.
ExpressionAtlasiQ93050. baseline and differential.
GenevisibleiQ93050. HS.

Family and domain databases

InterProiIPR002490. V-ATPase_116kDa_su.
IPR026028. V-type_ATPase_116kDa_su_euka.
[Graphical view]
PANTHERiPTHR11629. PTHR11629. 1 hit.
PfamiPF01496. V_ATPase_I. 1 hit.
[Graphical view]
PIRSFiPIRSF001293. ATP6V0A1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiVPP1_HUMAN
AccessioniPrimary (citable) accession number: Q93050
Secondary accession number(s): B7Z3B7, Q8N5G7, Q9NSX0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: February 15, 2005
Last modified: November 30, 2016
This is version 153 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.