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Q93050 (VPP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
V-type proton ATPase 116 kDa subunit a isoform 1

Short name=V-ATPase 116 kDa isoform a1
Alternative name(s):
Clathrin-coated vesicle/synaptic vesicle proton pump 116 kDa subunit
Vacuolar adenosine triphosphatase subunit Ac116
Vacuolar proton pump subunit 1
Vacuolar proton translocating ATPase 116 kDa subunit a isoform 1
Gene names
Name:ATP6V0A1
Synonyms:ATP6N1, ATP6N1A, VPP1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length837 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Required for assembly and activity of the vacuolar ATPase. Potential role in differential targeting and regulation of the enzyme for a specific organelle By similarity.

Subunit structure

The V-ATPase is a heteromultimeric enzyme composed of at least thirteen different subunits. It has a membrane peripheral V1 sector for ATP hydrolysis and an integral V0 for proton translocation. The V1 sector comprises subunits A-H, whereas V0 includes subunits a, d, c, c', and c''.

Subcellular location

Cytoplasmic vesicle membrane; Multi-pass membrane protein. Melanosome. Note: Coated vesicle. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Ref.7 Ref.8

Sequence similarities

Belongs to the V-ATPase 116 kDa subunit family.

Ontologies

Keywords
   Biological processHydrogen ion transport
Ion transport
Transport
   Cellular componentCytoplasmic vesicle
Membrane
   Coding sequence diversityAlternative splicing
   DomainTransmembrane
Transmembrane helix
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processATP hydrolysis coupled proton transport

Inferred from electronic annotation. Source: InterPro

cellular iron ion homeostasis

Traceable author statement. Source: Reactome

insulin receptor signaling pathway

Traceable author statement. Source: Reactome

interaction with host

Traceable author statement. Source: Reactome

phagosome maturation

Traceable author statement. Source: Reactome

transferrin transport

Traceable author statement. Source: Reactome

transmembrane transport

Traceable author statement. Source: Reactome

   Cellular_componentGolgi apparatus

Inferred from direct assay. Source: HPA

cytoplasm

Inferred from direct assay. Source: HPA

endosome membrane

Traceable author statement. Source: Reactome

extracellular vesicular exosome

Inferred from direct assay PubMed 19056867PubMed 23376485. Source: UniProt

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

intracellular membrane-bounded organelle

Inferred from direct assay. Source: HPA

melanosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from direct assay. Source: HPA

phagocytic vesicle membrane

Traceable author statement. Source: Reactome

plasma membrane

Inferred from direct assay PubMed 17360703. Source: UniProtKB

vacuolar proton-transporting V-type ATPase, V0 domain

Inferred from electronic annotation. Source: InterPro

   Molecular_functionATPase binding

Inferred from physical interaction PubMed 17360703. Source: UniProtKB

hydrogen ion transmembrane transporter activity

Inferred from electronic annotation. Source: InterPro

protein binding

Inferred from physical interaction PubMed 12649290. Source: HGNC

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q93050-2)

Also known as: I;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q93050-1)

Also known as: II;

The sequence of this isoform differs from the canonical sequence as follows:
     705-710: Missing.
Isoform 3 (identifier: Q93050-3)

The sequence of this isoform differs from the canonical sequence as follows:
     141-141: E → EAELHHQQ
     705-710: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 837837V-type proton ATPase 116 kDa subunit a isoform 1
PRO_0000119211

Regions

Topological domain1 – 388388Cytoplasmic Potential
Transmembrane389 – 40719Helical; Potential
Topological domain408 – 4092Vacuolar Potential
Transmembrane410 – 42617Helical; Potential
Topological domain427 – 44115Cytoplasmic Potential
Transmembrane442 – 47130Helical; Potential
Topological domain472 – 53463Vacuolar Potential
Transmembrane535 – 55420Helical; Potential
Topological domain555 – 57218Cytoplasmic Potential
Transmembrane573 – 59321Helical; Potential
Topological domain594 – 63845Vacuolar Potential
Transmembrane639 – 65820Helical; Potential
Topological domain659 – 72466Cytoplasmic Potential
Transmembrane725 – 74925Helical; Potential
Topological domain750 – 77021Vacuolar Potential
Transmembrane771 – 80939Helical; Potential
Topological domain810 – 83728Cytoplasmic Potential

Amino acid modifications

Modified residue3641Phosphotyrosine By similarity

Natural variations

Alternative sequence1411E → EAELHHQQ in isoform 3.
VSP_043532
Alternative sequence705 – 7106Missing in isoform 2 and isoform 3.
VSP_012814

Experimental info

Sequence conflict750 – 7512QL → HV in CAA96077. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (I) [UniParc].

Last modified February 15, 2005. Version 3.
Checksum: 80E4842CF6ADEE44

FASTA83796,413
        10         20         30         40         50         60 
MGELFRSEEM TLAQLFLQSE AAYCCVSELG ELGKVQFRDL NPDVNVFQRK FVNEVRRCEE 

        70         80         90        100        110        120 
MDRKLRFVEK EIRKANIPIM DTGENPEVPF PRDMIDLEAN FEKIENELKE INTNQEALKR 

       130        140        150        160        170        180 
NFLELTELKF ILRKTQQFFD EMADPDLLEE SSSLLEPSEM GRGTPLRLGF VAGVINRERI 

       190        200        210        220        230        240 
PTFERMLWRV CRGNVFLRQA EIENPLEDPV TGDYVHKSVF IIFFQGDQLK NRVKKICEGF 

       250        260        270        280        290        300 
RASLYPCPET PQERKEMASG VNTRIDDLQM VLNQTEDHRQ RVLQAAAKNI RVWFIKVRKM 

       310        320        330        340        350        360 
KAIYHTLNLC NIDVTQKCLI AEVWCPVTDL DSIQFALRRG TEHSGSTVPS ILNRMQTNQT 

       370        380        390        400        410        420 
PPTYNKTNKF TYGFQNIVDA YGIGTYREIN PAPYTIITFP FLFAVMFGDF GHGILMTLFA 

       430        440        450        460        470        480 
VWMVLRESRI LSQKNENEMF STVFSGRYII LLMGVFSMYT GLIYNDCFSK SLNIFGSSWS 

       490        500        510        520        530        540 
VRPMFTYNWT EETLRGNPVL QLNPALPGVF GGPYPFGIDP IWNIATNKLT FLNSFKMKMS 

       550        560        570        580        590        600 
VILGIIHMLF GVSLSLFNHI YFKKPLNIYF GFIPEIIFMT SLFGYLVILI FYKWTAYDAH 

       610        620        630        640        650        660 
TSENAPSLLI HFINMFLFSY PESGYSMLYS GQKGIQCFLV VVALLCVPWM LLFKPLVLRR 

       670        680        690        700        710        720 
QYLRRKHLGT LNFGGIRVGN GPTEEDAEII QHDQLSTHSE DADEPSEDEV FDFGDTMVHQ 

       730        740        750        760        770        780 
AIHTIEYCLG CISNTASYLR LWALSLAHAQ LSEVLWTMVI HIGLSVKSLA GGLVLFFFFT 

       790        800        810        820        830 
AFATLTVAIL LIMEGLSAFL HALRLHWVEF QNKFYSGTGF KFLPFSFEHI REGKFEE 

« Hide

Isoform 2 (II) [UniParc].

Checksum: B16170B568A3D586
Show »

FASTA83195,756
Isoform 3 [UniParc].

Checksum: BE9A648B592B7C67
Show »

FASTA83896,600

References

« Hide 'large scale' references
[1]"Sequence and alternative splicing in human 115 kDa subunit of vacuolar-type H(+)-ATPase."
Fehr C.N., Gillies R.J., Wang H.-Y., Ullrich A.
Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Tissue: Pancreas.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Hippocampus and Placenta.
[3]"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. expand/collapse author list , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[6]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 675-837 (ISOFORM 2).
Tissue: Testis.
[7]"Proteomic analysis of early melanosomes: identification of novel melanosomal proteins."
Basrur V., Yang F., Kushimoto T., Higashimoto Y., Yasumoto K., Valencia J., Muller J., Vieira W.D., Watabe H., Shabanowitz J., Hearing V.J., Hunt D.F., Appella E.
J. Proteome Res. 2:69-79(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Tissue: Melanoma.
[8]"Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes."
Chi A., Valencia J.C., Hu Z.-Z., Watabe H., Yamaguchi H., Mangini N.J., Huang H., Canfield V.A., Cheng K.C., Yang F., Abe R., Yamagishi S., Shabanowitz J., Hearing V.J., Wu C., Appella E., Hunt D.F.
J. Proteome Res. 5:3135-3144(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Tissue: Melanoma.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z71460 mRNA. Translation: CAA96077.1.
AK295682 mRNA. Translation: BAH12153.1.
AK316305 mRNA. Translation: BAH14676.1.
AC067852 Genomic DNA. No translation available.
AC107993 Genomic DNA. No translation available.
CH471152 Genomic DNA. Translation: EAW60830.1.
BC032398 mRNA. Translation: AAH32398.1.
AL137683 mRNA. Translation: CAB70874.1.
CCDSCCDS11426.1. [Q93050-1]
CCDS45683.1. [Q93050-3]
CCDS45684.1. [Q93050-2]
PIRT46449.
RefSeqNP_001123492.1. NM_001130020.1. [Q93050-3]
NP_001123493.1. NM_001130021.1. [Q93050-2]
NP_005168.2. NM_005177.3. [Q93050-1]
UniGeneHs.463074.

3D structure databases

ProteinModelPortalQ93050.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid107018. 22 interactions.
IntActQ93050. 7 interactions.
MINTMINT-3049850.
STRING9606.ENSP00000264649.

Protein family/group databases

TCDB3.A.2.2.4. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

PTM databases

PhosphoSiteQ93050.

Polymorphism databases

DMDM59803038.

Proteomic databases

MaxQBQ93050.
PaxDbQ93050.
PRIDEQ93050.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000264649; ENSP00000264649; ENSG00000033627. [Q93050-3]
ENST00000343619; ENSP00000342951; ENSG00000033627. [Q93050-2]
ENST00000393829; ENSP00000377415; ENSG00000033627. [Q93050-1]
GeneID535.
KEGGhsa:535.
UCSCuc002hzq.3. human. [Q93050-1]
uc002hzr.3. human. [Q93050-2]
uc002hzs.3. human. [Q93050-3]

Organism-specific databases

CTD535.
GeneCardsGC17P040610.
H-InvDBHIX0013842.
HGNCHGNC:865. ATP6V0A1.
HPAHPA022144.
MIM192130. gene.
neXtProtNX_Q93050.
PharmGKBPA25146.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1269.
HOGENOMHOG000037059.
HOVERGENHBG014606.
KOK02154.
OMAVIPRERM.
OrthoDBEOG74FF04.
PhylomeDBQ93050.
TreeFamTF300346.

Enzyme and pathway databases

BioCycMetaCyc:ENSG00000033627-MONOMER.
ReactomeREACT_111102. Signal Transduction.
REACT_116125. Disease.
REACT_15518. Transmembrane transport of small molecules.

Gene expression databases

ArrayExpressQ93050.
BgeeQ93050.
CleanExHS_ATP6V0A1.
GenevestigatorQ93050.

Family and domain databases

InterProIPR002490. V-ATPase_116kDa_su.
IPR026028. V-type_ATPase_116kDa_su_euka.
[Graphical view]
PANTHERPTHR11629. PTHR11629. 1 hit.
PfamPF01496. V_ATPase_I. 1 hit.
[Graphical view]
PIRSFPIRSF001293. ATP6V0A1. 1 hit.
ProtoNetSearch...

Other

ChiTaRSATP6V0A1. human.
GeneWikiATPase,_H%2B_transporting,_lysosomal_V0_subunit_a1.
GenomeRNAi535.
NextBio2223.
PROQ93050.
SOURCESearch...

Entry information

Entry nameVPP1_HUMAN
AccessionPrimary (citable) accession number: Q93050
Secondary accession number(s): B7Z3B7, Q8N5G7, Q9NSX0
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: February 15, 2005
Last modified: July 9, 2014
This is version 129 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM