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Protein

Glutamine--fructose-6-phosphate aminotransferase [isomerizing]

Gene

nodM

Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the production of the root hair deformation (HAD) factor specifically on medicago.By similarity

Catalytic activityi

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2Nucleophile; for GATase activityBy similarity1
Active sitei603For Fru-6P isomerization activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminotransferase, Transferase
Biological processNodulation

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (EC:2.6.1.16)
Short name:
GFAT
Alternative name(s):
Nodulation protein M
Gene namesi
Name:nodM
Ordered Locus Names:RA0482
ORF Names:SMa0878
Encoded oniPlasmid pSymA (megaplasmid 1)0 Publication
OrganismiRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Taxonomic identifieri266834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
Proteomesi
  • UP000001976 Componenti: Plasmid pSymA

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001354372 – 608Glutamine--fructose-6-phosphate aminotransferase [isomerizing]Add BLAST607

Structurei

3D structure databases

ProteinModelPortaliQ92ZK3.
SMRiQ92ZK3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 217Glutamine amidotransferase type-2Add BLAST216
Domaini281 – 422SIS 1Add BLAST142
Domaini456 – 598SIS 2Add BLAST143

Keywords - Domaini

Glutamine amidotransferase, Repeat

Phylogenomic databases

eggNOGiENOG4105C46. Bacteria.
COG0449. LUCA.
HOGENOMiHOG000258896.
KOiK00820.
OMAiRLDSSTC.

Family and domain databases

CDDicd05008. SIS_GlmS_GlmD_1. 1 hit.
cd05009. SIS_GlmS_GlmD_2. 1 hit.
Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_00164. GlmS. 1 hit.
InterProiView protein in InterPro
IPR017932. GATase_2_dom.
IPR035466. GlmS/AgaS_SIS.
IPR035490. GlmS/FrlB_SIS.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
PANTHERiPTHR10937:SF15. PTHR10937:SF15. 1 hit.
PfamiView protein in Pfam
PF01380. SIS. 2 hits.
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01135. glmS. 1 hit.
PROSITEiView protein in PROSITE
PS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q92ZK3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGIVGIVGN QPVSERLVEA LKRLEYRGYD SAGVATIDAG TLQRRRAEGK
60 70 80 90 100
LVNLESRLRE EPLAGTIGIA HTRWATHGAP TERNAHPHFT EGVAVVHNGI
110 120 130 140 150
IENFAELKDE LAAGGAEFQT ETDTEVVAHL LTKYRRDGLG RREAMHAMLK
160 170 180 190 200
RVKGAYALAV LFEDDPSTIM AARNGPPLAI GHGSGEMFLG SDAIALAPFT
210 220 230 240 250
NEITYLIDGD WAVIGKTGVH IFDFDGNVVE RPRQISTAAA FLVDKGNHRH
260 270 280 290 300
FMEKEIYEQP EVIAHALGHY VNFIENRVVP ISDAIDFGKV PSLAISACGT
310 320 330 340 350
AYLAGLIGKY WFERYARLPV EIDVASEFRY REIPLSPQSA ALFISQSGET
360 370 380 390 400
ADTLASLRYC KEHGLKIGAV VNARESTIAR ESDAVFPILA GPEIGVASTK
410 420 430 440 450
AFTCQLAVLA ALAVGAGKAR GTISGEEEQA LVKSLAEMPR IMGQVLNSIQ
460 470 480 490 500
PKIESLSREL SKCHDVLYLG RGTSFPLAME GALKLKEISY IHAEGYAAGE
510 520 530 540 550
LKHGPIALID ENMPVIVIAP HDRFFDKTVS NMQEVAARGG RIILITDEKG
560 570 580 590 600
AAASKLDTMH TIVLPEVDEI IAPMIFSLPL QLLAYHTAVF MGTDVDQPRN

LAKSVTVE
Length:608
Mass (Da):65,919
Last modified:January 23, 2007 - v3
Checksum:i91BC90051FEC23F4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006469 Genomic DNA. Translation: AAK65140.1.
PIRiB95322.
RefSeqiNP_435728.1. NC_003037.1.
WP_010967462.1. NC_003037.1.

Genome annotation databases

EnsemblBacteriaiAAK65140; AAK65140; SMa0878.
GeneIDi1235518.
KEGGisme:SMa0878.
PATRICifig|266834.11.peg.492.

Similar proteinsi

Entry informationi

Entry nameiNODM_RHIME
AccessioniPrimary (citable) accession number: Q92ZK3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: January 23, 2007
Last modified: August 30, 2017
This is version 101 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid, Reference proteome