Reviewed, UniProtKB/Swiss-Prot Q92ZJ6 (DNE22_RHIME)

Names and origin

Protein names

Recommended name:
    Error-prone DNA polymerase 2
    EC=2.7.7.7

Gene names

Name:	dnaE2-2
Ordered Locus Names:	RA0489
ORF Names:	SMa0892

Encoded on

Plasmid pSymA (megaplasmid 1)

Organism

Rhizobium meliloti (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

382 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	1087 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity. HAMAP MF_01902
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	Cytoplasm Probable HAMAP MF_01902.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1087	1087	Error-prone DNA polymerase 2 HAMAP MF_01902		PRO_0000103396

Sequences

	Sequence		Length	Mass (Da)
	Q92ZJ6-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 65E008BB06E2F98B	FASTA	1,087	121,796
	10 20 30 40 50 60 MRYAELQVTT HFSFLRAASS AEELFATARL MGIEALGVVD RNSLAGIVRA LEASRATGLR 70 80 90 100 110 120 LVVGCRLDLQ DGMSILVYPT DRAAYSRLTR LLTLGKGRGG KANCIIHFDD VALYAEGLIG 130 140 150 160 170 180 ILVPDLADEV CAVQLRKIAE VFGDRAYVSL CLRRRPNDQL QLHELTNLAV KHRVKTIVTN 190 200 210 220 230 240 DVLFHEHGRR QLQDVVTCIR TGMTIDDVGF ERERHADRYL KPPEEMARLF PAYPEALART 250 260 270 280 290 300 MEIVERCRFS LEELVYQYPE EALILGMTAQ QSLQHYTWEG VRARYPEGLP THVEKTIRHE 310 320 330 340 350 360 LALIETMKYA PYFLTVFSIV RYARSQGILC QGRGSAANSA VCYVLGITSI DPETNDLLFE 370 380 390 400 410 420 RFVSQERDEP PDIDVDFEHE RREEVIQWIY KTYGHDKAAL CSTVTRYRAK GAIRDVGKAL 430 440 450 460 470 480 DLPEDLIRTL SSGIWSWSET VGERQVRELG LNPDDRRLTL TLRLAQQLMG APRNLSQHPG 490 500 510 520 530 540 GFVLTHDRLD DLVPIEPATM ADRQVIEWDK DDIEALKFLK VDVLALGMLT CMAKAFALIS 550 560 570 580 590 600 EHKHEDIDLA TIPQEDPATY AMIRKADTLG TFQIESRAQM SMLPRMKPRT FYDLVIQVAI 610 620 630 640 650 660 VRPGPIQGDM VHPYLRRREG KEKVEYPTPE LEAVLHKTLG VPLFQESAMR VAMVCAGFTG 670 680 690 700 710 720 GEADQLRKSM ATFKFTGGVS RFKDKLVNGM IRNGYTKEFA EKTFSQLEGF GSYGFPESHA 730 740 750 760 770 780 ASFALIAYAS NYIKCYFPDV FCAALLNSQP MGFYAPAQIV RDAREHGVEV RPICINRSRW 790 800 810 820 830 840 DCMLEPIDGS GGHAVRLGMR LVRGLATADA ARIVAARADE PFTSVDDMWR RSGVPVASLV 850 860 870 880 890 900 ELAEADAFLP SLSLERRDAL WAIKALRDEP LPLFTAAADR EARAIAEQEE PEVELRQMTD 910 920 930 940 950 960 GQNVVEDYSH TGLTLREHPL RFMRDDLAKR RIVTCAQAMT AHDGQWLMAA GLVLVRQRPG 970 980 990 1000 1010 1020 SAKGVMFITI EDETGIANIV VWPKLFERSR RVVLGASMMA INGRIQREGE VVHLVAQQLF 1030 1040 1050 1060 1070 1080 DLSADLSSLA ERDGAFRPPT GRGDEFAHGS PGSADSRGKA PPGVRARDIL VPDLHIDTLK IKSRNFQ

References

[1]

"Nucleotide sequence and predicted functions of the entire Sinorhizobium meliloti pSymA megaplasmid." Barnett M.J., Fisher R.F., Jones T., Komp C., Abola A.P., Barloy-Hubler F., Bowser L., Capela D., Galibert F., Gouzy J., Gurjal M., Hong A., Huizar L., Hyman R.W., Kahn D., Kahn M.L., Kalman S., Keating D.H. , Palm C., Peck M.C., Surzycki R., Wells D.H., Yeh K.-C., Davis R.W., Federspiel N.A., Long S.R. Proc. Natl. Acad. Sci. U.S.A. 98:9883-9888(2001) [PubMed: 11481432] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006469 Genomic DNA. Translation: AAK65147.1.
PIR	A95323.
RefSeq	NP_435735.1.
3D structure databases
SMR	Q92ZJ6. Positions 2-865.
ModBase	Search...
Genome annotation databases
GeneID	1235525.
GenomeReviews	Gene locus RA0489 in contig AE006469_GR.
KEGG	sme:SMa0892.
NMPDR	fig|266834.1.peg.489.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734490.
OMA	LATIPQE.
Enzyme and pathway databases
BioCyc	SMEL266834:SMA0892-MONOMER.
BRENDA	2.7.7.7. 142.
Family and domain databases
HAMAP	MF_01902. DNApol_error_prone. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR004365. NA_bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. Pol/His_phosphatase_N. IPR016195. Pol/histidinol_Pase-like. IPR004805. PolC_alpha. [Graphical view]
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00594. polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNE22_RHIME

Accession

Primary (citable) accession number: Q92ZJ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	December 1, 2001
Last modified:	February 9, 2010
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)