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Q92Z36

- NAPA_RHIME

UniProt

Q92Z36 - NAPA_RHIME

Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster.UniRule annotation
    Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi50 – 501Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi53 – 531Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi57 – 571Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi85 – 851Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. electron carrier activity Source: UniProtKB-HAMAP
    3. iron ion binding Source: UniProtKB-HAMAP
    4. molybdenum ion binding Source: InterPro
    5. nitrate reductase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
    2. nitrate assimilation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Nitrate assimilation, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

    Enzyme and pathway databases

    BioCyciSMEL266834:GJF6-5682-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
    Gene namesi
    Name:napAUniRule annotation
    Ordered Locus Names:RA0674
    ORF Names:SMa1236
    Encoded oniPlasmid pSymA (megaplasmid 1)0 Publication
    OrganismiRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
    Taxonomic identifieri266834 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
    ProteomesiUP000001976: Plasmid pSymA

    Subcellular locationi

    Periplasm UniRule annotation

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3131Tat-type signalUniRule annotationAdd
    BLAST
    Chaini32 – 834803Periplasmic nitrate reductasePRO_0000045998Add
    BLAST

    Post-translational modificationi

    Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

    Interactioni

    Subunit structurei

    Interacts with NapB.UniRule annotation

    Protein-protein interaction databases

    STRINGi266834.SMa1236.

    Structurei

    3D structure databases

    ProteinModelPortaliQ92Z36.
    SMRiQ92Z36. Positions 43-830.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini43 – 99574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0243.
    HOGENOMiHOG000031441.
    KOiK02567.
    OMAiQMVYNLH.
    OrthoDBiEOG6CVV7G.

    Family and domain databases

    HAMAPiMF_01630. Nitrate_reduct.
    InterProiIPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    [Graphical view]
    PfamiPF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view]
    SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    TIGRFAMsiTIGR01706. NAPA. 1 hit.
    PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q92Z36-1 [UniParc]FASTAAdd to Basket

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    MTGELTRREM LKAHAAGIAA ATAGIALPAA AQPVPGGVEA LQITWSKAPC    50
    RFCGTGCGVM VGVKEGQVVA THGDMQAEVN RGLNCIKGYF LSKIMYGTDR 100
    LKTPLLRKRN GAFAKDGEFE PVSWDEAFDV MAEQAKKVLK DKGPTAVGMF 150
    GSGQWTIFEG YAATKLMRAG FRSNNLDPNA RHCMASAAYA FMRTFGMDEP 200
    MGCYDDFEHA DAFVLWGSNM AEMHPILWTR LADRRLGHEH VKVAVLSTFT 250
    HRSMDLADIP IVFKPGTDLA ILNYIANHII QTGRVNEDFV RKHTTFMVGA 300
    TDIGYGLRPD NPLEVKAVNA KDAAKMTPSD FESFKSFVSE YTLDKVVELT 350
    GVEAGFLEQL ADLYADPKRK VMSLWTMGFN QHVRGVWVNQ MVYNLHLLTG 400
    KISEPGNSPF SLTGQPSACG TAREVGTFAH RLPADMTVTN PEHRKHAEEI 450
    WRIPHGIIPE KPGYHAVEQD RMLKDGKLNF YWVQVNNNVQ AAPNTQNETY 500
    QGYRNPDNFI VVSDVYPTIT AMSADLILPA AMWVEKEGAY GNAERRTHVW 550
    HQLVDAPGEA RSDLWQMVEF SKRFTTDEVW STDILDANPG YRGKTLYDVL 600
    FKNGNVDSFP ASEINKEYAN REAEAFGFYI QKGLFEEYAS FGRGHGHDLA 650
    PYDRYHDERG LRWPVVDGKE TLWRYREGYD PYVKPGEGVK FYGRPDGKAV 700
    ILAVPYEPPA ESPDDEYNVW LVTGRVLEHW HSGSMTMRVP ELYKAFPGAV 750
    CFMNAGDARD RGINQGAEVR IVSRRGEIRA RVETRGRNRM PPGVIFVPWF 800
    DASRLINKVT LDATDPISKQ TDFKKCAVKI VSVA 834
    Length:834
    Mass (Da):93,247
    Last modified:December 1, 2001 - v1
    Checksum:i2A1B8F0090454A8D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE006469 Genomic DNA. Translation: AAK65332.1.
    PIRiB95346.
    RefSeqiNP_435920.1. NC_003037.1.

    Genome annotation databases

    EnsemblBacteriaiAAK65332; AAK65332; SMa1236.
    GeneIDi1235710.
    KEGGisme:SMa1236.
    PATRICi23627830. VBISinMel96828_0694.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE006469 Genomic DNA. Translation: AAK65332.1 .
    PIRi B95346.
    RefSeqi NP_435920.1. NC_003037.1.

    3D structure databases

    ProteinModelPortali Q92Z36.
    SMRi Q92Z36. Positions 43-830.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 266834.SMa1236.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK65332 ; AAK65332 ; SMa1236 .
    GeneIDi 1235710.
    KEGGi sme:SMa1236.
    PATRICi 23627830. VBISinMel96828_0694.

    Phylogenomic databases

    eggNOGi COG0243.
    HOGENOMi HOG000031441.
    KOi K02567.
    OMAi QMVYNLH.
    OrthoDBi EOG6CVV7G.

    Enzyme and pathway databases

    BioCyci SMEL266834:GJF6-5682-MONOMER.

    Family and domain databases

    HAMAPi MF_01630. Nitrate_reduct.
    InterProi IPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    [Graphical view ]
    Pfami PF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view ]
    SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    TIGRFAMsi TIGR01706. NAPA. 1 hit.
    PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 1021.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 1021.

    Entry informationi

    Entry nameiNAPA_RHIME
    AccessioniPrimary (citable) accession number: Q92Z36
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 7, 2006
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Plasmid, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3