Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite.UniRule annotation

Catalytic activityi

2 ferrocytochrome + nitrate + 2 H+ = 2 ferricytochrome + nitrite.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi50Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi53Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi57Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi85Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei87Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei154Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei179Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei183Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei377Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei381Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei487Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei536Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei563Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei799Substrate; via amide nitrogenUniRule annotation1
Binding sitei807Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei824Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Nitrate assimilation, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.9.6.1UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:RA0674
ORF Names:SMa1236
Encoded oniPlasmid pSymA (megaplasmid 1)0 Publication
OrganismiRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Taxonomic identifieri266834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
Proteomesi
  • UP000001976 Componenti: Plasmid pSymA

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31Tat-type signalUniRule annotationAdd BLAST31
ChainiPRO_000004599832 – 834Periplasmic nitrate reductaseUniRule annotationAdd BLAST803

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Component of the periplasmic nitrate reductase NapAB complex composed of NapA and NapB.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ92Z36.
SMRiQ92Z36.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini43 – 994Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni216 – 223Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation8
Regioni247 – 251Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation5
Regioni266 – 268Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation3
Regioni513 – 514Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation2
Regioni723 – 732Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation10

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000031441.
KOiK02567.
OMAiTTDEVWP.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct_NapA. 1 hit.
InterProiView protein in InterPro
IPR009010. Asp_de-COase-like_dom_sf.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
PANTHERiPTHR11615:SF123. PTHR11615:SF123. 1 hit.
PfamiView protein in Pfam
PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
SMARTiView protein in SMART
SM00926. Molybdop_Fe4S4. 1 hit.
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
PROSITEiView protein in PROSITE
PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q92Z36-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTGELTRREM LKAHAAGIAA ATAGIALPAA AQPVPGGVEA LQITWSKAPC
60 70 80 90 100
RFCGTGCGVM VGVKEGQVVA THGDMQAEVN RGLNCIKGYF LSKIMYGTDR
110 120 130 140 150
LKTPLLRKRN GAFAKDGEFE PVSWDEAFDV MAEQAKKVLK DKGPTAVGMF
160 170 180 190 200
GSGQWTIFEG YAATKLMRAG FRSNNLDPNA RHCMASAAYA FMRTFGMDEP
210 220 230 240 250
MGCYDDFEHA DAFVLWGSNM AEMHPILWTR LADRRLGHEH VKVAVLSTFT
260 270 280 290 300
HRSMDLADIP IVFKPGTDLA ILNYIANHII QTGRVNEDFV RKHTTFMVGA
310 320 330 340 350
TDIGYGLRPD NPLEVKAVNA KDAAKMTPSD FESFKSFVSE YTLDKVVELT
360 370 380 390 400
GVEAGFLEQL ADLYADPKRK VMSLWTMGFN QHVRGVWVNQ MVYNLHLLTG
410 420 430 440 450
KISEPGNSPF SLTGQPSACG TAREVGTFAH RLPADMTVTN PEHRKHAEEI
460 470 480 490 500
WRIPHGIIPE KPGYHAVEQD RMLKDGKLNF YWVQVNNNVQ AAPNTQNETY
510 520 530 540 550
QGYRNPDNFI VVSDVYPTIT AMSADLILPA AMWVEKEGAY GNAERRTHVW
560 570 580 590 600
HQLVDAPGEA RSDLWQMVEF SKRFTTDEVW STDILDANPG YRGKTLYDVL
610 620 630 640 650
FKNGNVDSFP ASEINKEYAN REAEAFGFYI QKGLFEEYAS FGRGHGHDLA
660 670 680 690 700
PYDRYHDERG LRWPVVDGKE TLWRYREGYD PYVKPGEGVK FYGRPDGKAV
710 720 730 740 750
ILAVPYEPPA ESPDDEYNVW LVTGRVLEHW HSGSMTMRVP ELYKAFPGAV
760 770 780 790 800
CFMNAGDARD RGINQGAEVR IVSRRGEIRA RVETRGRNRM PPGVIFVPWF
810 820 830
DASRLINKVT LDATDPISKQ TDFKKCAVKI VSVA
Length:834
Mass (Da):93,247
Last modified:December 1, 2001 - v1
Checksum:i2A1B8F0090454A8D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006469 Genomic DNA. Translation: AAK65332.1.
PIRiB95346.
RefSeqiNP_435920.1. NC_003037.1.
WP_010967653.1. NC_003037.1.

Genome annotation databases

EnsemblBacteriaiAAK65332; AAK65332; SMa1236.
GeneIDi1235710.
25011345.
KEGGisme:SMa1236.
PATRICifig|266834.11.peg.694.

Similar proteinsi

Entry informationi

Entry nameiNAPA_RHIME
AccessioniPrimary (citable) accession number: Q92Z36
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: December 1, 2001
Last modified: January 31, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families