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Q92Z36 (NAPA_RHIME) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:RA0674
ORF Names:SMa1236
Encoded onPlasmid pSymA (megaplasmid 1)
OrganismRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) [Complete proteome] [HAMAP]
Taxonomic identifier266834 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium

Protein attributes

Sequence length834 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131Tat-type signal Potential
Chain32 – 834803Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_0000045998

Regions

Domain43 – 99574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding501Iron-sulfur (4Fe-4S) By similarity
Metal binding531Iron-sulfur (4Fe-4S) By similarity
Metal binding571Iron-sulfur (4Fe-4S) By similarity
Metal binding851Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q92Z36 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 2A1B8F0090454A8D

FASTA83493,247
        10         20         30         40         50         60 
MTGELTRREM LKAHAAGIAA ATAGIALPAA AQPVPGGVEA LQITWSKAPC RFCGTGCGVM 

        70         80         90        100        110        120 
VGVKEGQVVA THGDMQAEVN RGLNCIKGYF LSKIMYGTDR LKTPLLRKRN GAFAKDGEFE 

       130        140        150        160        170        180 
PVSWDEAFDV MAEQAKKVLK DKGPTAVGMF GSGQWTIFEG YAATKLMRAG FRSNNLDPNA 

       190        200        210        220        230        240 
RHCMASAAYA FMRTFGMDEP MGCYDDFEHA DAFVLWGSNM AEMHPILWTR LADRRLGHEH 

       250        260        270        280        290        300 
VKVAVLSTFT HRSMDLADIP IVFKPGTDLA ILNYIANHII QTGRVNEDFV RKHTTFMVGA 

       310        320        330        340        350        360 
TDIGYGLRPD NPLEVKAVNA KDAAKMTPSD FESFKSFVSE YTLDKVVELT GVEAGFLEQL 

       370        380        390        400        410        420 
ADLYADPKRK VMSLWTMGFN QHVRGVWVNQ MVYNLHLLTG KISEPGNSPF SLTGQPSACG 

       430        440        450        460        470        480 
TAREVGTFAH RLPADMTVTN PEHRKHAEEI WRIPHGIIPE KPGYHAVEQD RMLKDGKLNF 

       490        500        510        520        530        540 
YWVQVNNNVQ AAPNTQNETY QGYRNPDNFI VVSDVYPTIT AMSADLILPA AMWVEKEGAY 

       550        560        570        580        590        600 
GNAERRTHVW HQLVDAPGEA RSDLWQMVEF SKRFTTDEVW STDILDANPG YRGKTLYDVL 

       610        620        630        640        650        660 
FKNGNVDSFP ASEINKEYAN REAEAFGFYI QKGLFEEYAS FGRGHGHDLA PYDRYHDERG 

       670        680        690        700        710        720 
LRWPVVDGKE TLWRYREGYD PYVKPGEGVK FYGRPDGKAV ILAVPYEPPA ESPDDEYNVW 

       730        740        750        760        770        780 
LVTGRVLEHW HSGSMTMRVP ELYKAFPGAV CFMNAGDARD RGINQGAEVR IVSRRGEIRA 

       790        800        810        820        830 
RVETRGRNRM PPGVIFVPWF DASRLINKVT LDATDPISKQ TDFKKCAVKI VSVA 

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE006469 Genomic DNA. Translation: AAK65332.1.
PIRB95346.
RefSeqNP_435920.1. NC_003037.1.

3D structure databases

ProteinModelPortalQ92Z36.
SMRQ92Z36. Positions 43-830.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING266834.SMa1236.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK65332; AAK65332; SMa1236.
GeneID1235710.
KEGGsme:SMa1236.
PATRIC23627830. VBISinMel96828_0694.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMARRTHVWH.
OrthoDBEOG6CVV7G.
ProtClustDBPRK13532.

Enzyme and pathway databases

BioCycSMEL266834:GJF6-5682-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_RHIME
AccessionPrimary (citable) accession number: Q92Z36
Entry history
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: December 1, 2001
Last modified: February 19, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families