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Protein

DNA polymerase IV 2

Gene

dinB2

Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII (By similarity).By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi8MagnesiumBy similarity1
Sitei13Substrate discriminationBy similarity1
Metal bindingi102MagnesiumBy similarity1
Active sitei103By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Mutator protein, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair, DNA replication

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase IV 2 (EC:2.7.7.7)
Short name:
Pol IV 2
Gene namesi
Name:dinB2
Ordered Locus Names:RA1274
ORF Names:SMa2355
Encoded oniPlasmid pSymA (megaplasmid 1)0 Publication
OrganismiRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Taxonomic identifieri266834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
Proteomesi
  • UP000001976 Componenti: Plasmid pSymA

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001739401 – 354DNA polymerase IV 2Add BLAST354

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ92XH8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 184UmuCAdd BLAST181

Sequence similaritiesi

Belongs to the DNA polymerase type-Y family.Curated
Contains 1 umuC domain.Curated

Phylogenomic databases

HOGENOMiHOG000082707.
KOiK02346.

Family and domain databases

Gene3Di3.30.1490.100. 1 hit.
HAMAPiMF_01113. DNApol_IV. 1 hit.
InterProiIPR017961. DNA_pol_Y-fam_little_finger.
IPR022880. DNApol_IV.
IPR024728. PolY_HhH_motif.
IPR001126. UmuC.
[Graphical view]
PfamiPF00817. IMS. 1 hit.
PF11799. IMS_C. 1 hit.
PF11798. IMS_HHH. 1 hit.
[Graphical view]
SUPFAMiSSF100879. SSF100879. 1 hit.
PROSITEiPS50173. UMUC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q92XH8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKIIHIDMD AFYASVEQRD NPELRGKPVA VGYPEARGVV AAASYEARKF
60 70 80 90 100
GVHSAMPSVT AKRKCPELIF VPHRFDVYRA VSRQIQAIFA EYTPLVEPLS
110 120 130 140 150
LDEAYLDVTE NFRGLKLATE IAEEIRGRIR AETHLTASAG VSYNKFLAKM
160 170 180 190 200
ASDQRKPDGL FVITPKHGPD FVQALPVKKF HGVGPATAEK MKRLGIETGA
210 220 230 240 250
DLKSRDLAFL QQHFGKSGPY FYWIARGIDE RKVKPDRIRK SIGAEDTFRE
260 270 280 290 300
DVHDLETARA GLKPLIDKVW HYCEASGIRG KTMTLKVKWA DFTQITRSKT
310 320 330 340 350
IVAPIASVAE MSEIAELLLS PIFPAPKGIR LLGVTLSSLD TVDDRSEPQL

ALAL
Length:354
Mass (Da):39,431
Last modified:August 2, 2002 - v2
Checksum:i341544D538CD1F18
GO

Sequence cautioni

The sequence AAK65932 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006469 Genomic DNA. Translation: AAK65932.2. Different initiation.
PIRiB95421.
RefSeqiNP_436520.4. NC_003037.1.

Genome annotation databases

EnsemblBacteriaiAAK65932; AAK65932; SMa2355.
GeneIDi1236311.
KEGGisme:SMa2355.
PATRICi23629102. VBISinMel96828_1329.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006469 Genomic DNA. Translation: AAK65932.2. Different initiation.
PIRiB95421.
RefSeqiNP_436520.4. NC_003037.1.

3D structure databases

ProteinModelPortaliQ92XH8.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK65932; AAK65932; SMa2355.
GeneIDi1236311.
KEGGisme:SMa2355.
PATRICi23629102. VBISinMel96828_1329.

Phylogenomic databases

HOGENOMiHOG000082707.
KOiK02346.

Family and domain databases

Gene3Di3.30.1490.100. 1 hit.
HAMAPiMF_01113. DNApol_IV. 1 hit.
InterProiIPR017961. DNA_pol_Y-fam_little_finger.
IPR022880. DNApol_IV.
IPR024728. PolY_HhH_motif.
IPR001126. UmuC.
[Graphical view]
PfamiPF00817. IMS. 1 hit.
PF11799. IMS_C. 1 hit.
PF11798. IMS_HHH. 1 hit.
[Graphical view]
SUPFAMiSSF100879. SSF100879. 1 hit.
PROSITEiPS50173. UMUC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDPO42_RHIME
AccessioniPrimary (citable) accession number: Q92XH8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: August 2, 2002
Last modified: November 30, 2016
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.