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Q92X16

- PDXA2_RHIME

UniProt

Q92X16 - PDXA2_RHIME

Protein

4-hydroxythreonine-4-phosphate dehydrogenase 2

Gene

pdxA2

Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the NAD(P)-dependent oxidation of 4-(phosphohydroxy)-L-threonine (HTP) into 2-amino-3-oxo-4-(phosphohydroxy)butyric acid which spontaneously decarboxylates to form 3-amino-2-oxopropyl phosphate (AHAP).UniRule annotation

    Catalytic activityi

    4-phosphonooxy-L-threonine + NAD+ = 3-amino-2-oxopropyl phosphate + CO2 + NADH.

    Cofactori

    Binds 1 divalent metal cation per subunit. Can use ions such as zinc, magnesium or cobalt.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei123 – 1231SubstrateUniRule annotation
    Binding sitei124 – 1241SubstrateUniRule annotation
    Metal bindingi153 – 1531Divalent metal cation; shared with dimeric partnerUniRule annotation
    Metal bindingi197 – 1971Divalent metal cation; shared with dimeric partnerUniRule annotation
    Metal bindingi252 – 2521Divalent metal cation; shared with dimeric partnerUniRule annotation
    Binding sitei260 – 2601SubstrateUniRule annotation
    Binding sitei269 – 2691SubstrateUniRule annotation
    Binding sitei278 – 2781SubstrateUniRule annotation

    GO - Molecular functioni

    1. 4-hydroxythreonine-4-phosphate dehydrogenase activity Source: UniProtKB-HAMAP
    2. cobalt ion binding Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP
    4. NAD binding Source: InterPro
    5. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. pyridoxal phosphate biosynthetic process Source: UniProtKB-HAMAP
    2. pyridoxine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Pyridoxine biosynthesis

    Keywords - Ligandi

    Cobalt, Magnesium, Metal-binding, NAD, NADP, Zinc

    Enzyme and pathway databases

    BioCyciSMEL266834:GJF6-3579-MONOMER.
    UniPathwayiUPA00244; UER00312.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    4-hydroxythreonine-4-phosphate dehydrogenase 2UniRule annotation (EC:1.1.1.262UniRule annotation)
    Alternative name(s):
    4-(phosphohydroxy)-L-threonine dehydrogenase 2UniRule annotation
    Gene namesi
    Name:pdxA2UniRule annotation
    Ordered Locus Names:RB0146
    ORF Names:SMb20146
    Encoded oniPlasmid pSymB (megaplasmid 2)0 Publication
    OrganismiRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
    Taxonomic identifieri266834 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
    ProteomesiUP000001976: Plasmid pSymB

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 3213214-hydroxythreonine-4-phosphate dehydrogenase 2PRO_0000188822Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi266834.SM_b20146.

    Structurei

    3D structure databases

    ProteinModelPortaliQ92X16.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PdxA family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1995.
    HOGENOMiHOG000221593.
    KOiK00097.
    OMAiYERPRIA.
    OrthoDBiEOG6GN6ZC.

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    HAMAPiMF_00536. PdxA.
    InterProiIPR024084. IsoPropMal-DH-like_dom.
    IPR005255. PdxA.
    [Graphical view]
    PfamiPF04166. PdxA. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00557. pdxA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q92X16-1 [UniParc]FASTAAdd to Basket

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    MSKIIGITMG DPCGVGPEIT VRSLAEMSAA DREATRIYGN LATLEAAREA    50
    LGLSVDLQPY VVDLTVEGAP LPWGSLSAVA GDAAFRFIER AVRDAEAGNI 100
    GCIVTAPINK EALNMAGHHY DGHTGMLRSL TGSSAAYMLL ASERLKVIHV 150
    STHVSLKEAI GRATTERVLA TIRAGNAHLK RIGYEHPRIA VAGINPHCGE 200
    NGLFGTEDDD QIGPAVAAAR EEGIDVQGPI SADTVFHRAY SGGFDLVVAQ 250
    YHDQGHIPIK LVAFDTAVNV SVDLPIDRTS VDHGTAFDIA GKGIANHGNL 300
    NSAIAYARKL VAGQASRKAA S 321
    Length:321
    Mass (Da):33,689
    Last modified:December 1, 2001 - v1
    Checksum:iF6C54F187F325110
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL591985 Genomic DNA. Translation: CAC48546.1.
    PIRiB95860.
    RefSeqiNP_436686.1. NC_003078.1.

    Genome annotation databases

    EnsemblBacteriaiCAC48546; CAC48546; SM_b20146.
    GeneIDi1236477.
    KEGGisme:SM_b20146.
    PATRICi23636679. VBISinMel96828_5061.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL591985 Genomic DNA. Translation: CAC48546.1 .
    PIRi B95860.
    RefSeqi NP_436686.1. NC_003078.1.

    3D structure databases

    ProteinModelPortali Q92X16.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 266834.SM_b20146.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAC48546 ; CAC48546 ; SM_b20146 .
    GeneIDi 1236477.
    KEGGi sme:SM_b20146.
    PATRICi 23636679. VBISinMel96828_5061.

    Phylogenomic databases

    eggNOGi COG1995.
    HOGENOMi HOG000221593.
    KOi K00097.
    OMAi YERPRIA.
    OrthoDBi EOG6GN6ZC.

    Enzyme and pathway databases

    UniPathwayi UPA00244 ; UER00312 .
    BioCyci SMEL266834:GJF6-3579-MONOMER.

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    HAMAPi MF_00536. PdxA.
    InterProi IPR024084. IsoPropMal-DH-like_dom.
    IPR005255. PdxA.
    [Graphical view ]
    Pfami PF04166. PdxA. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00557. pdxA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing endosymbiont Sinorhizobium meliloti."
      Finan T.M., Weidner S., Wong K., Buhrmester J., Chain P., Vorhoelter F.J., Hernandez-Lucas I., Becker A., Cowie A., Gouzy J., Golding B., Puehler A.
      Proc. Natl. Acad. Sci. U.S.A. 98:9889-9894(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 1021.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 1021.

    Entry informationi

    Entry nameiPDXA2_RHIME
    AccessioniPrimary (citable) accession number: Q92X16
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 31, 2002
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The active site is located at the dimer interface.UniRule annotation

    Keywords - Technical termi

    Complete proteome, Plasmid, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3