4-hydroxythreonine-4-phosphate dehydrogenase 2 - Rhizobium meliloti (strain 1021) (Ensifer meliloti)

Contribute

Send feedback

Read comments (?) or add your own

Q92X16 (PDXA2_RHIME) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 78. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
4-hydroxythreonine-4-phosphate dehydrogenase 2
EC=1.1.1.262

Alternative name(s):
4-(phosphohydroxy)-L-threonine dehydrogenase 2

Gene names

Name:	pdxA2
Ordered Locus Names:	RB0146
ORF Names:	SMb20146

Encoded on

Plasmid pSymB (megaplasmid 2)

Organism

Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

266834 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium ›

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NAD(P)-dependent oxidation of 4-(phosphohydroxy)-L-threonine (HTP) into 2-amino-3-oxo-4-(phosphohydroxy)butyric acid which spontaneously decarboxylates to form 3-amino-2-oxopropyl phosphate (AHAP) By similarity. HAMAP-Rule MF_00536
Catalytic activity	4-phosphonooxy-L-threonine + NAD⁺ = 3-amino-2-oxopropyl phosphate + CO₂ + NADH. HAMAP-Rule MF_00536
Cofactor	Binds 1 divalent metal cation per subunit. Can use ions such as zinc, magnesium or cobalt By similarity.
Pathway	Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 4/5. HAMAP-Rule MF_00536
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	The active site is located at the dimer interface By similarity. HAMAP-Rule MF_00536
Sequence similarities	Belongs to the PdxA family.

Ontologies

Keywords
Biological process	Pyridoxine biosynthesis
Cellular component	Cytoplasm
Ligand	Cobalt Magnesium Metal-binding NAD NADP Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological_process	pyridoxal phosphate biosynthetic process Inferred from electronic annotation. Source: HAMAP pyridoxine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4-hydroxythreonine-4-phosphate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP NAD binding Inferred from electronic annotation. Source: InterPro cobalt ion binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 321

321

4-hydroxythreonine-4-phosphate dehydrogenase 2 HAMAP-Rule MF_00536

PRO_0000188822

Sites

Metal binding

153

Divalent metal cation; shared with dimeric partner By similarity

Metal binding

197

Divalent metal cation; shared with dimeric partner By similarity

Metal binding

252

Divalent metal cation; shared with dimeric partner By similarity

Binding site

123

Substrate By similarity

Binding site

124

Substrate By similarity

Binding site

260

Substrate By similarity

Binding site

269

Substrate By similarity

Binding site

278

Substrate By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q92X16 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: F6C54F187F325110
Show »

FASTA

321

33,689

        10         20         30         40         50         60 
MSKIIGITMG DPCGVGPEIT VRSLAEMSAA DREATRIYGN LATLEAAREA LGLSVDLQPY 

        70         80         90        100        110        120 
VVDLTVEGAP LPWGSLSAVA GDAAFRFIER AVRDAEAGNI GCIVTAPINK EALNMAGHHY 

       130        140        150        160        170        180 
DGHTGMLRSL TGSSAAYMLL ASERLKVIHV STHVSLKEAI GRATTERVLA TIRAGNAHLK 

       190        200        210        220        230        240 
RIGYEHPRIA VAGINPHCGE NGLFGTEDDD QIGPAVAAAR EEGIDVQGPI SADTVFHRAY 

       250        260        270        280        290        300 
SGGFDLVVAQ YHDQGHIPIK LVAFDTAVNV SVDLPIDRTS VDHGTAFDIA GKGIANHGNL 

       310        320 
NSAIAYARKL VAGQASRKAA S

« Hide

References

[1]

"The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing endosymbiont Sinorhizobium meliloti."
Finan T.M., Weidner S., Wong K., Buhrmester J., Chain P., Vorhoelter F.J., Hernandez-Lucas I., Becker A., Cowie A., Gouzy J., Golding B., Puehler A.
Proc. Natl. Acad. Sci. U.S.A. 98:9889-9894(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1021.

[2]

"The composite genome of the legume symbiont Sinorhizobium meliloti."
Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F., Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G., Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P., Cowie A.

Batut J.
Science 293:668-672(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1021.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL591985 Genomic DNA. Translation: CAC48546.1.
PIR	B95860.
RefSeq	NP_436686.1. NC_003078.1.
3D structure databases
ProteinModelPortal	Q92X16.
ModBase	Search...
Protein-protein interaction databases
STRING	266834.SM_b20146.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAC48546; CAC48546; SM_b20146.
GeneID	1236477.
KEGG	sme:SM_b20146.
PATRIC	23636679. VBISinMel96828_5061.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1995.
HOGENOM	HOG000221593.
KO	K00097.
OMA	YMLLASE.
ProtClustDB	CLSK806862.
Enzyme and pathway databases
BioCyc	SMEL266834:GJF6-3579-MONOMER.
UniPathway	UPA00244; UER00312.
Family and domain databases
Gene3D	3.40.718.10. 1 hit.
HAMAP	MF_00536. PdxA.
InterPro	IPR024084. IsoPropMal-DH-like_dom. IPR005255. PyrdxlP_synth_PdxA. [Graphical view]
Pfam	PF04166. PdxA. 1 hit. [Graphical view]
TIGRFAMs	TIGR00557. pdxA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PDXA2_RHIME

Accession

Primary (citable) accession number: Q92X16

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 31, 2002
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents