ID   HIUH2_RHIME             Reviewed;         120 AA.
AC   Q92UG5;
DT   02-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT   02-MAY-2002, sequence version 2.
DT   24-JAN-2024, entry version 114.
DE   RecName: Full=5-hydroxyisourate hydrolase 2;
DE            Short=HIU hydrolase 2;
DE            Short=HIUHase 2;
DE            EC=3.5.2.17;
GN   OrderedLocusNames=RB1166; ORFNames=SMb20872;
OS   Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS   meliloti).
OG   Plasmid pSymB (megaplasmid 2).
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=266834;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11481431; DOI=10.1073/pnas.161294698;
RA   Finan T.M., Weidner S., Wong K., Buhrmester J., Chain P., Vorhoelter F.J.,
RA   Hernandez-Lucas I., Becker A., Cowie A., Gouzy J., Golding B., Puehler A.;
RT   "The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing
RT   endosymbiont Sinorhizobium meliloti.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:9889-9894(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11474104; DOI=10.1126/science.1060966;
RA   Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA   Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA   Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA   Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA   Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA   Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA   Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA   Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA   Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA   Wong K., Yeh K.-C., Batut J.;
RT   "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL   Science 293:668-672(2001).
CC   -!- FUNCTION: Catalyzes the hydrolysis of 5-hydroxyisourate (HIU) to 2-oxo-
CC       4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5-hydroxyisourate + H2O = 5-hydroxy-2-oxo-4-ureido-2,5-
CC         dihydro-1H-imidazole-5-carboxylate + H(+); Xref=Rhea:RHEA:23736,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:18072,
CC         ChEBI:CHEBI:58639; EC=3.5.2.17;
CC   -!- SUBUNIT: Homotetramer. {ECO:0000250}.
CC   -!- MISCELLANEOUS: HIU hydrolysis also occurs spontaneously, but more
CC       slowly.
CC   -!- SIMILARITY: Belongs to the transthyretin family. 5-hydroxyisourate
CC       hydrolase subfamily. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAC49566.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AL591985; CAC49566.1; ALT_INIT; Genomic_DNA.
DR   PIR; F95987; F95987.
DR   RefSeq; NP_437706.1; NC_003078.1.
DR   RefSeq; WP_013850504.1; NC_003078.1.
DR   AlphaFoldDB; Q92UG5; -.
DR   SMR; Q92UG5; -.
DR   EnsemblBacteria; CAC49566; CAC49566; SM_b20872.
DR   GeneID; 61601114; -.
DR   KEGG; sme:SM_b20872; -.
DR   PATRIC; fig|266834.11.peg.6092; -.
DR   eggNOG; COG2351; Bacteria.
DR   HOGENOM; CLU_115536_1_1_5; -.
DR   OrthoDB; 9792386at2; -.
DR   Proteomes; UP000001976; Plasmid pSymB.
DR   GO; GO:0033971; F:hydroxyisourate hydrolase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006144; P:purine nucleobase metabolic process; IEA:UniProtKB-KW.
DR   CDD; cd05822; TLP_HIUase; 1.
DR   Gene3D; 2.60.40.180; Transthyretin/hydroxyisourate hydrolase domain; 1.
DR   InterPro; IPR014306; Hydroxyisourate_hydrolase.
DR   InterPro; IPR023418; Thyroxine_BS.
DR   InterPro; IPR023416; Transthyretin/HIU_hydrolase_d.
DR   InterPro; IPR036817; Transthyretin/HIU_hydrolase_sf.
DR   InterPro; IPR023419; Transthyretin_CS.
DR   NCBIfam; TIGR02962; hdxy_isourate; 1.
DR   PANTHER; PTHR10395:SF7; 5-HYDROXYISOURATE HYDROLASE; 1.
DR   PANTHER; PTHR10395; URICASE AND TRANSTHYRETIN-RELATED; 1.
DR   Pfam; PF00576; Transthyretin; 1.
DR   SUPFAM; SSF49472; Transthyretin (synonym: prealbumin); 1.
DR   PROSITE; PS00768; TRANSTHYRETIN_1; 1.
DR   PROSITE; PS00769; TRANSTHYRETIN_2; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Plasmid; Purine metabolism; Reference proteome.
FT   CHAIN           1..120
FT                   /note="5-hydroxyisourate hydrolase 2"
FT                   /id="PRO_0000050617"
FT   BINDING         10
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         48
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         117
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   120 AA;  13114 MW;  1A12DCC4F2E46232 CRC64;
     MSKSGRLTTH VLDTALGRPA HGLKIDLYRL EGDARHLIRT VHTNSDGRVD GPLMEGAGFA
     TGTYELVFHA GDYFRSAGVK LPDPAFLELV PLRFGIADAD SHYHVPLLLS PYGYSTYRGS
//