Q92UG5 (HIUH2_RHIME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-hydroxyisourate hydrolase 2
Short name=HIU hydrolase 2
Short name=HIUHase 2
EC=3.5.2.17

Gene names

Ordered Locus Names:	RB1166
ORF Names:	SMb20872

Encoded on

Plasmid pSymB (megaplasmid 2)

Organism

Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

266834 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium › Rhizobium meliloti

Protein attributes

Sequence length	120 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the hydrolysis of 5-hydroxyisourate (HIU) to 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU) By similarity.
Catalytic activity	5-hydroxyisourate + H2O = 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate.
Subunit structure	Homotetramer By similarity.
Miscellaneous	HIU hydrolysis also occurs spontaneously, but more slowly.
Sequence similarities	Belongs to the transthyretin family. 5-hydroxyisourate hydrolase subfamily.
Sequence caution	The sequence CAC49566.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Purine metabolism
Molecular function	Hydrolase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological_process	purine nucleobase metabolic process Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: InterPro
Molecular_function	hydroxyisourate hydrolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 120	120	5-hydroxyisourate hydrolase 2		PRO_0000050617
Sites
	Binding site	10	1	Substrate By similarity
	Binding site	48	1	Substrate By similarity
	Binding site	117	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q92UG5 [UniParc]. Last modified May 2, 2002. Version 2. Checksum: 1A12DCC4F2E46232	FASTA	120	13,114
	10 20 30 40 50 60 MSKSGRLTTH VLDTALGRPA HGLKIDLYRL EGDARHLIRT VHTNSDGRVD GPLMEGAGFA 70 80 90 100 110 120 TGTYELVFHA GDYFRSAGVK LPDPAFLELV PLRFGIADAD SHYHVPLLLS PYGYSTYRGS

References

[1]

"The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing endosymbiont Sinorhizobium meliloti." Finan T.M., Weidner S., Wong K., Buhrmester J., Chain P., Vorhoelter F.J., Hernandez-Lucas I., Becker A., Cowie A., Gouzy J., Golding B., Puehler A. Proc. Natl. Acad. Sci. U.S.A. 98:9889-9894(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

[2]

"The composite genome of the legume symbiont Sinorhizobium meliloti." Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F., Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G., Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P., Cowie A. , Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S., Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I., Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S., Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C., Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R., Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H., Wong K., Yeh K.-C., Batut J. Science 293:668-672(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL591985 Genomic DNA. Translation: CAC49566.1. Different initiation.
PIR	F95987.
RefSeq	NP_437706.1. NC_003078.1.
3D structure databases
ProteinModelPortal	Q92UG5.
SMR	Q92UG5. Positions 5-120.
ModBase	Search...
Protein-protein interaction databases
STRING	266834.SM_b20872.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAC49566; CAC49566; SM_b20872.
GeneID	1237497.
KEGG	sme:SM_b20872.
PATRIC	23638742. VBISinMel96828_6092.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2351.
KO	K07127.
ProtClustDB	CLSK808246.
Enzyme and pathway databases
BioCyc	SMEL266834:GJF6-4599-MONOMER.
Family and domain databases
Gene3D	2.60.40.180. 1 hit.
InterPro	IPR014306. Hydroxyisourate_hydrolase. IPR023418. Thyroxine_BS. IPR023416. Transthyretin/HIU_hydrolase_SF. IPR023419. Transthyretin_CS. [Graphical view]
Pfam	PF00576. Transthyretin. 1 hit. [Graphical view]
SUPFAM	SSF49472. Transthyretin. 1 hit.
TIGRFAMs	TIGR02962. hdxy_isourate. 1 hit.
PROSITE	PS00768. TRANSTHYRETIN_1. 1 hit. PS00769. TRANSTHYRETIN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HIUH2_RHIME

Accession

Primary (citable) accession number: Q92UG5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2002
Last sequence update:	May 2, 2002
Last modified:	May 1, 2013
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program