Q92TY6 (NADE_RHIME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Synonyms:	nadE1
Ordered Locus Names:	RB1357
ORF Names:	SMb20649

Encoded on

Plasmid pSymB (megaplasmid 2)

Organism

Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

266834 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_0000152189
Regions
	Nucleotide binding	47 – 54	8	ATP By similarity
Sites
	Active site	49	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q92TY6 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 5BD444642974AFC1	FASTA	334	36,913
	10 20 30 40 50 60 MNIRPDQNRL VFSADTLKID EAAEADRIVA GLRAQLRSLR KRGLVLGLSG GIDSSVSVAL 70 80 90 100 110 120 AVRAVGAKNV FCLFMPENDS DPESLRLGRL VAETFGVEAV VEDIGPTLDA MGCYQRRDAF 130 140 150 160 170 180 IRELVPDYGP GWASKIVIAN ALEGDGYNIS SLVVQDPEGK QTKLRMPPSV YLGIVAATNM 190 200 210 220 230 240 KQRTRKQIEY YHADRLNFAV LGTPNRLEYD QGFFVKNGDG AADVKPIAHL YKSQVYALAG 250 260 270 280 290 300 HLGIPEEIRR RPPTTDTYSL EQTQEEFYFS LPYDRMDLCL FGLNNGLSAD EVGRAANLGV 310 320 330 AQVKRVWADI AAKRKATRYL HLGPQLVQPV EEIE

References

[1]

"The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing endosymbiont Sinorhizobium meliloti." Finan T.M., Weidner S., Wong K., Buhrmester J., Chain P., Vorhoelter F.J., Hernandez-Lucas I., Becker A., Cowie A., Gouzy J., Golding B., Puehler A. Proc. Natl. Acad. Sci. U.S.A. 98:9889-9894(2001) [PubMed: 11481431] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

[2]

"The composite genome of the legume symbiont Sinorhizobium meliloti." Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F., Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G., Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P., Cowie A. , Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S., Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I., Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S., Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C., Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R., Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H., Wong K., Yeh K.-C., Batut J. Science 293:668-672(2001) [PubMed: 11474104] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL591985 Genomic DNA. Translation: CAC49757.1.
PIR	E96011.
RefSeq	NP_437897.1. NC_003078.1.
3D structure databases
ProteinModelPortal	Q92TY6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1237689.
GenomeReviews	Gene locus RB1357 in contig AL591985_GR.
NMPDR	fig|266834.1.peg.5992.
PATRIC	23639114. VBISinMel96828_6277.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG351567.
OMA	AHLYKSQ.
ProtClustDB	PRK00876.
Enzyme and pathway databases
BioCyc	SMEL266834:SMB20649-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
Pfam	PF02540. NAD_synthase. 2 hits. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_RHIME

Accession

Primary (citable) accession number: Q92TY6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	December 1, 2001
Last modified:	January 25, 2012
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program