Reviewed, UniProtKB/Swiss-Prot Q92TA4 (MASZ_RHIME)

Names and origin

Protein names

Recommended name:
    Malate synthase G
    EC=2.3.3.9

Gene names

Name:	glcB
Ordered Locus Names:	R00062
ORF Names:	SMc02581

Organism

Rhizobium meliloti (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

382 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	723 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP MF_00641
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP MF_00641
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	malate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 723	723	Malate synthase G HAMAP MF_00641		PRO_0000166898
Sites
	Active site	338	1	Proton acceptor By similarity
	Active site	629	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q92TA4-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: A0E95E8A5164BB58	FASTA	723	78,854
	10 20 30 40 50 60 MDRVEKYGLQ IDAGLHRFLV EEAMPGTGVD ADRFFSAFSD LVHDLGPKNR ALLVKRDELQ 70 80 90 100 110 120 ARLDGWYREH GAPVDMEAYE AFLREIGYLL PEGPDFYVST ANVDSEIATI AGPQLVVPVM 130 140 150 160 170 180 NARYALNAAN ARWGSLYDAL YGTDAIAETD GAERGKGYNP KRGAKVIAWA REFLDASAPL 190 200 210 220 230 240 AAGRWSDAKS FSVEGATLTV TLADGTKSAP RNSVQFAGYA GDPAAPSEIV LRRNGLHIVI 250 260 270 280 290 300 VLDATTPIGK ADAAGISDVV LESAITTIMD CEDSIAAVDA EDKVLVYRNW LGLMKGDLEE 310 320 330 340 350 360 EVTKGGRAFT RRLNPDRAYT APDGATLTLP GRSLMLVRNV GHLMTNPAVL DRDGEEVPEG 370 380 390 400 410 420 LMDAMVTALI ALHDIGRNGR RANSRSGSMY VVKPKMHGPE EVAFACEIFA RVEAALGLPA 430 440 450 460 470 480 NAMKMGIMDE ERRTTVNLKE CIRAARERVV FINTGFLDRT GDEIHTSMEA GPMIRKGDMK 490 500 510 520 530 540 QAPWISAYEN WNVDVGLECG LSGHAQIGKG MWAMPDLMAA MLEQKIVHPK AGANTAWVPS 550 560 570 580 590 600 PTAATLHATH YHRVNVAEVQ ASLKSRTRAK LADILSVPVA ARPNWTEEEI QRELDNNAQG 610 620 630 640 650 660 ILGYVVRWVD QGVGCSKVPD INNVGLMEDR ATLRISAQHM ANWLHHGIVS EAQIVETMQR 670 680 690 700 710 720 MAAIVDGQNA GDPNYQPMAG RFEESIAFQA ALDLVLKGRE QPNGYTEPVL HRRRLELKAK QGA

References

[1]

"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021." Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F. Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed: 11481430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL591688 Genomic DNA. Translation: CAC41449.1.
RefSeq	NP_384168.1.
3D structure databases
HSSP	HSSP built from PDB template 1N8I based on UniProtKB Q50596.
ModBase	Search...
Genome annotation databases
GeneID	1231687.
GenomeReviews	Gene locus R00062 in contig AL591688_GR.
KEGG	sme:SMc02581.
NMPDR	fig|266834.1.peg.1356.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q92TA4.
OMA	PKMHGPD.
Enzyme and pathway databases
BioCyc	SMEL266834:SMC02581-MON.
BRENDA	2.3.3.9. 142.
Family and domain databases
HAMAP	MF_00641. [Tree]
InterPro	IPR011076. Malate_synth-like_core. IPR001465. Malate_synthase. IPR006253. Malate_synthG. [Graphical view]
PANTHER	PTHR21631:SF1. Malate_synthase. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01345. malate_syn_G. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MASZ_RHIME

Accession

Primary (citable) accession number: Q92TA4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 30, 2003
Last sequence update:	December 1, 2001
Last modified:	November 3, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)