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Protein

Malate synthase G

Gene

glcB

Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: glyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Malate synthase G (glcB)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei116Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Binding sitei274Acetyl-CoAUniRule annotation1
Binding sitei311Acetyl-CoAUniRule annotation1
Active sitei338Proton acceptorUniRule annotation1
Binding sitei338GlyoxylateUniRule annotation1
Metal bindingi430MagnesiumUniRule annotation1
Binding sitei430GlyoxylateUniRule annotation1
Metal bindingi458MagnesiumUniRule annotation1
Binding sitei539Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Active sitei629Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:R00062
ORF Names:SMc02581
OrganismiRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Taxonomic identifieri266834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
Proteomesi
  • UP000001976 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001668981 – 723Malate synthase GAdd BLAST723

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei615Cysteine sulfenic acid (-SOH)UniRule annotation1

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi266834.SMc02581.

Structurei

3D structure databases

ProteinModelPortaliQ92TA4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni123 – 124Acetyl-CoA bindingUniRule annotation2
Regioni455 – 458Glyoxylate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QP3. Bacteria.
COG2225. LUCA.
HOGENOMiHOG000220740.
KOiK01638.
OMAiTEPVLHA.

Family and domain databases

CDDicd00728. malate_synt_G. 1 hit.
Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G. 1 hit.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

Q92TA4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDRVEKYGLQ IDAGLHRFLV EEAMPGTGVD ADRFFSAFSD LVHDLGPKNR
60 70 80 90 100
ALLVKRDELQ ARLDGWYREH GAPVDMEAYE AFLREIGYLL PEGPDFYVST
110 120 130 140 150
ANVDSEIATI AGPQLVVPVM NARYALNAAN ARWGSLYDAL YGTDAIAETD
160 170 180 190 200
GAERGKGYNP KRGAKVIAWA REFLDASAPL AAGRWSDAKS FSVEGATLTV
210 220 230 240 250
TLADGTKSAP RNSVQFAGYA GDPAAPSEIV LRRNGLHIVI VLDATTPIGK
260 270 280 290 300
ADAAGISDVV LESAITTIMD CEDSIAAVDA EDKVLVYRNW LGLMKGDLEE
310 320 330 340 350
EVTKGGRAFT RRLNPDRAYT APDGATLTLP GRSLMLVRNV GHLMTNPAVL
360 370 380 390 400
DRDGEEVPEG LMDAMVTALI ALHDIGRNGR RANSRSGSMY VVKPKMHGPE
410 420 430 440 450
EVAFACEIFA RVEAALGLPA NAMKMGIMDE ERRTTVNLKE CIRAARERVV
460 470 480 490 500
FINTGFLDRT GDEIHTSMEA GPMIRKGDMK QAPWISAYEN WNVDVGLECG
510 520 530 540 550
LSGHAQIGKG MWAMPDLMAA MLEQKIVHPK AGANTAWVPS PTAATLHATH
560 570 580 590 600
YHRVNVAEVQ ASLKSRTRAK LADILSVPVA ARPNWTEEEI QRELDNNAQG
610 620 630 640 650
ILGYVVRWVD QGVGCSKVPD INNVGLMEDR ATLRISAQHM ANWLHHGIVS
660 670 680 690 700
EAQIVETMQR MAAIVDGQNA GDPNYQPMAG RFEESIAFQA ALDLVLKGRE
710 720
QPNGYTEPVL HRRRLELKAK QGA
Length:723
Mass (Da):78,854
Last modified:December 1, 2001 - v1
Checksum:iA0E95E8A5164BB58
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL591688 Genomic DNA. Translation: CAC41449.1.
RefSeqiNP_384168.1. NC_003047.1.
WP_010968325.1. NC_003047.1.

Genome annotation databases

EnsemblBacteriaiCAC41449; CAC41449; SMc02581.
GeneIDi1231687.
KEGGisme:SMc02581.
PATRICi23629277. VBISinMel96828_1416.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL591688 Genomic DNA. Translation: CAC41449.1.
RefSeqiNP_384168.1. NC_003047.1.
WP_010968325.1. NC_003047.1.

3D structure databases

ProteinModelPortaliQ92TA4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi266834.SMc02581.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC41449; CAC41449; SMc02581.
GeneIDi1231687.
KEGGisme:SMc02581.
PATRICi23629277. VBISinMel96828_1416.

Phylogenomic databases

eggNOGiENOG4107QP3. Bacteria.
COG2225. LUCA.
HOGENOMiHOG000220740.
KOiK01638.
OMAiTEPVLHA.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Family and domain databases

CDDicd00728. malate_synt_G. 1 hit.
Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G. 1 hit.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMASZ_RHIME
AccessioniPrimary (citable) accession number: Q92TA4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.