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Q92SN3 (GSHB_RHIME) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione synthetase

EC=6.3.2.3
Alternative name(s):
GSH synthetase
Short name=GSH-S
Short name=GSHase
Glutathione synthase
Gene names
Name:gshB
Ordered Locus Names:R00338
ORF Names:SMc00419
OrganismRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) [Complete proteome] [HAMAP]
Taxonomic identifier266834 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium

Protein attributes

Sequence length315 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP-Rule MF_00162

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. HAMAP-Rule MF_00162

Sequence similarities

Belongs to the prokaryotic GSH synthase family.

Contains 1 ATP-grasp domain.

Ontologies

Keywords
   Biological processGlutathione biosynthesis
   LigandATP-binding
Magnesium
Manganese
Metal-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcytosol

Inferred from electronic annotation. Source: InterPro

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutathione synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

manganese ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 315315Glutathione synthetase HAMAP-Rule MF_00162
PRO_0000197481

Regions

Domain127 – 311185ATP-grasp
Nucleotide binding153 – 20957ATP By similarity

Sites

Metal binding2821Magnesium or manganese By similarity
Metal binding2841Magnesium or manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
Q92SN3 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 32666BD118EB4087

FASTA31535,317
        10         20         30         40         50         60 
MAGIVNVGVQ MDHVSGITIA GDSTFAMSLE AQARGYRLFH YTPDKLSLRD GRVYATAQQM 

        70         80         90        100        110        120 
QLRDVRGDHF TLGEDERIDL STMDVILLRQ DPPFDMAYIT STHLLERLHP KTLVVNDPAW 

       130        140        150        160        170        180 
VRNSPEKIFV TEFPDLMPKT LITRDPDEIA RFRQEMGDII LKPLYGNGGA GVFHSARDDR 

       190        200        210        220        230        240 
NFSSLLEMFA QMFREPYIAQ EYLPAVRKGD KRILLVDGEP VGAINRVPAE HDSRSNMHVG 

       250        260        270        280        290        300 
GRAEATDLTA REREICTRIG PALKERGFLF VGIDVIGDYL TEINVTSPTG IREVKKFGGA 

       310 
DVASLLWDAI EKKRA 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL591688 Genomic DNA. Translation: CAC41775.1.
RefSeqNP_384444.1. NC_003047.1.

3D structure databases

ProteinModelPortalQ92SN3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING266834.SMc00419.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAC41775; CAC41775; SMc00419.
GeneID1231972.
KEGGsme:SMc00419.
PATRIC23629879. VBISinMel96828_1709.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0189.
HOGENOMHOG000265022.
KOK01920.
OMAPTCFQEI.
OrthoDBEOG6WMHWB.
ProtClustDBPRK05246.

Enzyme and pathway databases

BioCycSMEL266834:GJF6-349-MONOMER.
UniPathwayUPA00142; UER00210.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00162. GSH_S.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamPF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01380. glut_syn. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGSHB_RHIME
AccessionPrimary (citable) accession number: Q92SN3
Entry history
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: December 1, 2001
Last modified: February 19, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways