Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q92SI9 (SYW_RHIME) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tryptophan--tRNA ligase

EC=6.1.1.2
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name=TrpRS
Gene names
Name:trpS
Ordered Locus Names:R00399
ORF Names:SMc01121
OrganismRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) [Complete proteome] [HAMAP]
Taxonomic identifier266834 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium

Protein attributes

Sequence length354 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp). HAMAP-Rule MF_00140

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00140

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00140.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtryptophanyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tryptophan-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 354354Tryptophan--tRNA ligase HAMAP-Rule MF_00140
PRO_0000136667

Regions

Motif14 – 229"HIGH" region HAMAP-Rule MF_00140
Motif217 – 2215"KMSKS" region HAMAP-Rule MF_00140

Sites

Binding site2201ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q92SI9 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: EEEA6F4BFC1AF959

FASTA35439,168
        10         20         30         40         50         60 
MNEFKPLVFS GVQPTGNLHL GNYLGAIRKF VALQENNDCI YCVVDLHSIT AQLVHEDLPG 

        70         80         90        100        110        120 
QIRSIAAAFI ASGIDPEKHI VFNQSAVPQH AELAWIFNCV ARIGWMNRMT QFKDKAGKDR 

       130        140        150        160        170        180 
ENASLGLLAY PSLMAADILV YRATHVPVGD DQKQHLELTR DIAQKFNIDF MEHIRRGGYG 

       190        200        210        220        230        240 
VDIVVGEEPI HAYFPPVEPL IGGPAPRVMS LRDGTKKMSK SDPSDLSRIN LMDDADTILK 

       250        260        270        280        290        300 
KIRKAKTDPD ALPSEADGLK DRPEAENLVG IYAALADRSK EEVLAEFGGQ QFSTFKPALA 

       310        320        330        340        350 
DLAVSVLSPI TGEMRRLMGD TAHIDAILRK GGERARERAE TTMREVREII GFLQ 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL591688 Genomic DNA. Translation: CAC41836.1.
RefSeqNP_384505.1. NC_003047.1.

3D structure databases

ProteinModelPortalQ92SI9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING266834.SMc01121.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAC41836; CAC41836; SMc01121.
GeneID1232033.
KEGGsme:SMc01121.
PATRIC23630005. VBISinMel96828_1772.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0180.
HOGENOMHOG000059940.
KOK01867.
OMAFAFYLAC.
OrthoDBEOG686NJQ.
ProtClustDBPRK00927.

Enzyme and pathway databases

BioCycSMEL266834:GJF6-410-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
HAMAPMF_00140_B. Trp_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERPTHR10055. PTHR10055. 1 hit.
PfamPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSPR01039. TRNASYNTHTRP.
TIGRFAMsTIGR00233. trpS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYW_RHIME
AccessionPrimary (citable) accession number: Q92SI9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: December 1, 2001
Last modified: February 19, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries