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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathway:iN(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei63 – 631S-adenosyl-L-methionineUniRule annotation
Binding sitei88 – 881S-adenosyl-L-methionineUniRule annotation
Binding sitei115 – 1151S-adenosyl-L-methionineUniRule annotation
Active sitei137 – 1371By similarity
Binding sitei137 – 1371S-adenosyl-L-methionineUniRule annotation
Binding sitei141 – 1411SubstrateUniRule annotation
Binding sitei173 – 1731SubstrateUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSMEL266834:GJF6-423-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:R00412
ORF Names:SMc01108
OrganismiRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Taxonomic identifieri266834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
ProteomesiUP000001976 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 232232tRNA (guanine-N(7)-)-methyltransferasePRO_0000171381Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi266834.SMc01108.

Structurei

3D structure databases

ProteinModelPortaliQ92SI3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni211 – 2144Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073969.
KOiK03439.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q92SI3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTETRGARAT EAFFGRRKGK PLRERQAAHL EHLLPLLKLD LEEPAPADLT
60 70 80 90 100
ALFPEPVERI RLEIGFGGGE HLIHRAAEDP ATGFIGVEPF VNSMAKLLGQ
110 120 130 140 150
IEAKAIRNIR LYDDDATQVL DWLPAASVDQ IDLLYPDPWP KRKHWKRRFV
160 170 180 190 200
SQINLDRFAR ILKPGGLFCF ASDIDSYINW TLIHCREHAA FEWTAERAAD
210 220 230
WLTPFAGWPS TRYEAKARRE GRSSAYLAFR RA
Length:232
Mass (Da):26,532
Last modified:May 29, 2007 - v2
Checksum:i5955B6D4DFCBDC99
GO

Sequence cautioni

The sequence CAC41849.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL591688 Genomic DNA. Translation: CAC41849.1. Different initiation.
RefSeqiNP_384518.1. NC_003047.1.

Genome annotation databases

EnsemblBacteriaiCAC41849; CAC41849; SMc01108.
GeneIDi1232046.
KEGGisme:SMc01108.
PATRICi23630031. VBISinMel96828_1785.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL591688 Genomic DNA. Translation: CAC41849.1. Different initiation.
RefSeqiNP_384518.1. NC_003047.1.

3D structure databases

ProteinModelPortaliQ92SI3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi266834.SMc01108.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC41849; CAC41849; SMc01108.
GeneIDi1232046.
KEGGisme:SMc01108.
PATRICi23630031. VBISinMel96828_1785.

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073969.
KOiK03439.
OrthoDBiEOG6K6VBC.

Enzyme and pathway databases

UniPathwayiUPA00989.
BioCyciSMEL266834:GJF6-423-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1021.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1021.

Entry informationi

Entry nameiTRMB_RHIME
AccessioniPrimary (citable) accession number: Q92SI3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: May 29, 2007
Last modified: January 7, 2015
This is version 67 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.