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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei63S-adenosyl-L-methionineUniRule annotation1
Binding sitei88S-adenosyl-L-methionineUniRule annotation1
Binding sitei115S-adenosyl-L-methionineUniRule annotation1
Active sitei137By similarity1
Binding sitei137S-adenosyl-L-methionineUniRule annotation1
Binding sitei141SubstrateUniRule annotation1
Binding sitei173SubstrateUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:R00412
ORF Names:SMc01108
OrganismiRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Taxonomic identifieri266834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
Proteomesi
  • UP000001976 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001713811 – 232tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST232

Proteomic databases

PRIDEiQ92SI3

Interactioni

Protein-protein interaction databases

STRINGi266834.SMc01108

Structurei

3D structure databases

ProteinModelPortaliQ92SI3
SMRiQ92SI3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni211 – 214Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZ1 Bacteria
COG0220 LUCA
HOGENOMiHOG000073969
KOiK03439

Family and domain databases

HAMAPiMF_01057 tRNA_methyltr_TrmB, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PANTHERiPTHR23417 PTHR23417, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit

Sequencei

Sequence statusi: Complete.

Q92SI3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTETRGARAT EAFFGRRKGK PLRERQAAHL EHLLPLLKLD LEEPAPADLT
60 70 80 90 100
ALFPEPVERI RLEIGFGGGE HLIHRAAEDP ATGFIGVEPF VNSMAKLLGQ
110 120 130 140 150
IEAKAIRNIR LYDDDATQVL DWLPAASVDQ IDLLYPDPWP KRKHWKRRFV
160 170 180 190 200
SQINLDRFAR ILKPGGLFCF ASDIDSYINW TLIHCREHAA FEWTAERAAD
210 220 230
WLTPFAGWPS TRYEAKARRE GRSSAYLAFR RA
Length:232
Mass (Da):26,532
Last modified:May 29, 2007 - v2
Checksum:i5955B6D4DFCBDC99
GO

Sequence cautioni

The sequence CAC41849 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL591688 Genomic DNA Translation: CAC41849.1 Different initiation.
RefSeqiNP_384518.1, NC_003047.1

Genome annotation databases

EnsemblBacteriaiCAC41849; CAC41849; SMc01108
GeneIDi1232046
KEGGisme:SMc01108
PATRICifig|266834.11.peg.1785

Similar proteinsi

Entry informationi

Entry nameiTRMB_RHIME
AccessioniPrimary (citable) accession number: Q92SI3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: May 29, 2007
Last modified: May 23, 2018
This is version 76 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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