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Q92SI3 (TRMB_RHIME) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:R00412
ORF Names:SMc01108
OrganismRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) [Complete proteome] [HAMAP]
Taxonomic identifier266834 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium ›

Protein attributes

Sequence length232 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the methyltransferase superfamily. TrmB family.

Sequence caution

The sequence CAC41849.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processRNA (guanine-N7)-methylation

Inferred from electronic annotation. Source: GOC

   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 232232tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171381

Regions

Region211 – 2144Substrate binding Potential

Sites

Binding site631S-adenosyl-L-methionine By similarity
Binding site881S-adenosyl-L-methionine By similarity
Binding site1151S-adenosyl-L-methionine By similarity
Binding site1371S-adenosyl-L-methionine By similarity
Binding site1411Substrate By similarity
Binding site1731Substrate Potential

Sequences

Sequence LengthMass (Da)Tools
Q92SI3 [UniParc].

Last modified May 29, 2007. Version 2.
Checksum: 5955B6D4DFCBDC99

FASTA23226,532
        10         20         30         40         50         60 
MTETRGARAT EAFFGRRKGK PLRERQAAHL EHLLPLLKLD LEEPAPADLT ALFPEPVERI 

        70         80         90        100        110        120 
RLEIGFGGGE HLIHRAAEDP ATGFIGVEPF VNSMAKLLGQ IEAKAIRNIR LYDDDATQVL 

       130        140        150        160        170        180 
DWLPAASVDQ IDLLYPDPWP KRKHWKRRFV SQINLDRFAR ILKPGGLFCF ASDIDSYINW 

       190        200        210        220        230 
TLIHCREHAA FEWTAERAAD WLTPFAGWPS TRYEAKARRE GRSSAYLAFR RA

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AL591688 Genomic DNA. Translation: CAC41849.1. Different initiation.
RefSeqNP_384518.1. NC_003047.1.

3D structure databases

ProteinModelPortalQ92SI3.
ModBaseSearch...

Protein-protein interaction databases

STRING266834.SMc01108.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAC41849; CAC41849; SMc01108.
GeneID1232046.
KEGGsme:SMc01108.
PATRIC23630031. VBISinMel96828_1785.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073969.
KOK03439.
ProtClustDBPRK00121.

Enzyme and pathway databases

BioCycSMEL266834:GJF6-423-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_RHIME
AccessionPrimary (citable) accession number: Q92SI3
Entry history
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: May 29, 2007
Last modified: May 1, 2013
This is version 59 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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