Phosphoribosylamine--glycine ligase - Rhizobium meliloti (strain 1021) (Ensifer meliloti)

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Q92RL0 (PUR2_RHIME) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 75. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Phosphoribosylamine--glycine ligase
EC=6.3.4.13

Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	R00858
ORF Names:	SMc00993

Organism

Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

266834 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium ›

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway purine nucleobase biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 423

423

Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138

PRO_0000151474

Regions

Domain

107 – 312

206

ATP-grasp

Nucleotide binding

133 – 193

ATP By similarity

Sites

Metal binding

282

Magnesium or manganese By similarity

Metal binding

284

Magnesium or manganese By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q92RL0 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 5E65E13B606D204B
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FASTA

423

44,324

        10         20         30         40         50         60 
MKVLLIGSGG REHALAWKIA QSPRLTKLYA APGNPGIAEE AAIVDLHTEN HEDVVDFCRT 

        70         80         90        100        110        120 
HAIDFVVVGP EAPLVAGLGD VLRAADIPTF GPSAAAAQLE GSKGFTKDLC ARYGIPTGAY 

       130        140        150        160        170        180 
RRFTDAEPAK AYIREEGAPI VIKADGLAAG KGVTVAMSLD EAFAAVDECF AGAFGAAGAE 

       190        200        210        220        230        240 
VVVEAYLDGE EASFFCLCDG KSVLPLASAQ DHKRVGDGDT GPNTGGMGAY SPAPVMTAEM 

       250        260        270        280        290        300 
VERTMKEIIE PTVRGMAESG YPFTGVFFAG LMITEKGPEL IEYNVRFGDP ECQVLMMRLE 

       310        320        330        340        350        360 
SDLLPLLYAA ATGTLEGMKA EWRDEAALTV VMASRGYPGA YEKDTPIAAL PDASAATKVF 

       370        380        390        400        410        420 
HAGTAMKGGG LVAAGGRVLN VTATGKTVGE AKDAAYAAVR DVSWENGFYR NDIGWRAVAR 


ETA

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References

[1]

"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021."
Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D.

Galibert F.
Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1021.

[2]

"The composite genome of the legume symbiont Sinorhizobium meliloti."
Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F., Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G., Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P., Cowie A.

Batut J.
Science 293:668-672(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1021.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL591688 Genomic DNA. Translation: CAC45430.1.
RefSeq	NP_384964.1. NC_003047.1.
3D structure databases
ProteinModelPortal	Q92RL0.
SMR	Q92RL0. Positions 1-420.
ModBase	Search...
Protein-protein interaction databases
STRING	266834.SMc00993.
Proteomic databases
ProMEX	Q92RL0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAC45430; CAC45430; SMc00993.
GeneID	1232499.
KEGG	sme:SMc00993.
PATRIC	23630975. VBISinMel96828_2251.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0151.
HOGENOM	HOG000033463.
KO	K01945.
OMA	MGAYTPL.
ProtClustDB	PRK00885.
Enzyme and pathway databases
BioCyc	SMEL266834:GJF6-877-MONOMER.
UniPathway	UPA00074; UER00125.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 1 hit. 3.40.50.20. 1 hit. 3.90.600.10. 1 hit.
HAMAP	MF_00138. GARS.
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR016185. PreATP-grasp_dom. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N. IPR011054. Rudment_hybrid_motif. [Graphical view]
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit. SSF51246. Rudmnt_hyb_motif. 1 hit.
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_RHIME

Accession

Primary (citable) accession number: Q92RL0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents