Reviewed, UniProtKB/Swiss-Prot Q92RL0 (PUR2_RHIME)

Names and origin

Protein names

Recommended name:
    Phosphoribosylamine--glycine ligase
    EC=6.3.4.13
Alternative name(s):
    GARS
    Glycinamide ribonucleotide synthetase
    Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	R00858
ORF Names:	SMc00993

Organism

Rhizobium meliloti (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

382 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP MF_00138
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity. HAMAP MF_00138
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 423	423	Phosphoribosylamine--glycine ligase HAMAP MF_00138		PRO_0000151474
Regions
	Domain	107 – 312	206	ATP-grasp
	Nucleotide binding	133 – 193	61	ATP By similarity
Sites
	Metal binding	282	1	Magnesium or manganese By similarity
	Metal binding	284	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q92RL0-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 5E65E13B606D204B	FASTA	423	44,324
	10 20 30 40 50 60 MKVLLIGSGG REHALAWKIA QSPRLTKLYA APGNPGIAEE AAIVDLHTEN HEDVVDFCRT 70 80 90 100 110 120 HAIDFVVVGP EAPLVAGLGD VLRAADIPTF GPSAAAAQLE GSKGFTKDLC ARYGIPTGAY 130 140 150 160 170 180 RRFTDAEPAK AYIREEGAPI VIKADGLAAG KGVTVAMSLD EAFAAVDECF AGAFGAAGAE 190 200 210 220 230 240 VVVEAYLDGE EASFFCLCDG KSVLPLASAQ DHKRVGDGDT GPNTGGMGAY SPAPVMTAEM 250 260 270 280 290 300 VERTMKEIIE PTVRGMAESG YPFTGVFFAG LMITEKGPEL IEYNVRFGDP ECQVLMMRLE 310 320 330 340 350 360 SDLLPLLYAA ATGTLEGMKA EWRDEAALTV VMASRGYPGA YEKDTPIAAL PDASAATKVF 370 380 390 400 410 420 HAGTAMKGGG LVAAGGRVLN VTATGKTVGE AKDAAYAAVR DVSWENGFYR NDIGWRAVAR ETA

References

[1]

"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021." Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F. Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed: 11481430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL591688 Genomic DNA. Translation: CAC45430.1.
RefSeq	NP_384964.1.
3D structure databases
SMR	Q92RL0. Positions 1-420.
ModBase	Search...
Genome annotation databases
GeneID	1232499.
GenomeReviews	Gene locus R00858 in contig AL591688_GR.
KEGG	sme:SMc00993.
NMPDR	fig|266834.1.peg.2152.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG404060.
OMA	FSLMAFV.
Enzyme and pathway databases
BioCyc	SMEL266834:SMC00993-MONOMER.
BRENDA	6.3.4.13. 142.
Family and domain databases
HAMAP	MF_00138. GARS. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N-dom. IPR011054. Rudment_hybrid_motif. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.90.600.10. Gars. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_RHIME

Accession

Primary (citable) accession number: Q92RL0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	December 1, 2001
Last modified:	February 9, 2010
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)