Reviewed, UniProtKB/Swiss-Prot Q92R32 (NADB_RHIME)

Names and origin

Protein names

Recommended name:
    L-aspartate oxidase
      Short name=LASPO
    EC=1.4.3.16
Alternative name(s):
    Quinolinate synthetase B

Gene names

Name:	nadB
Ordered Locus Names:	R01095
ORF Names:	SMc02599

Organism

Rhizobium meliloti (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

382 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	532 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of L-aspartate to iminoaspartate.
Catalytic activity	L-aspartate + O2 = iminosuccinate + H2O2.
Cofactor	FAD.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (oxidase route): step 1/1.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-aspartate oxidase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 532	532	L-aspartate oxidase		PRO_0000184397
Regions
	Nucleotide binding	18 – 32	15	FAD Potential
Sites
	Active site	242	1	By similarity
	Active site	261	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q92R32-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 7C3321F90921E8E9	FASTA	532	56,646
	10 20 30 40 50 60 MLTDHFRPQS FNGIDDIVIV GGGLAGLFCA LKLAPRPVTI LAAAPIGYGA SSAWAQGGIA 70 80 90 100 110 120 AAMSKGDTFE KHVADTVAAG AGIVDEKMTR MMVAEGPARI HDLLEFGVPF DRDLEGKLLL 130 140 150 160 170 180 SREAAHSERR IVRVKGDMAG KSIMEALIAA VRNTPSIRVI EGYVVEELVR EGRFISGVIA 190 200 210 220 230 240 RPDAGQSKSR VSFPARAVVL CSGGVGHLYA VTTNPWEACG QGVGMAARAG AIIADPEFVQ 250 260 270 280 290 300 FHPTAIDIGK DPAPLATEAL RGDGATLINS EGRRFMLDIH PDGELAPRDI VSRGVFAEVQ 310 320 330 340 350 360 AGRGAFLDCT KAVGRHFPEM FPTVYASCMA AGIDPVRQPI PVTPAVHYHM GGVLTDGEGR 370 380 390 400 410 420 TSIDGLWAAG EVTSTGVHGA NRLASNSLLE AVVFAARIAE NIKGSLPAPK LTAWGDNAGE 430 440 450 460 470 480 NDDPVTVEDS PPFKVLRQLM SDCVGVVRTR ESLTRAIRGI AELERTNKRL RFANIATTAK 490 500 510 520 530 LIAIAALQRT ESRGGHFRAD CPDERPEWRR RTYLTLAQAE RLAAEVAESE PA

References

[1]

"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021." Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F. Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed: 11481430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL591688 Genomic DNA. Translation: CAC45674.1.
RefSeq	NP_385201.1.
3D structure databases
HSSP	HSSP built from PDB template 1D4D based on UniProtKB P83223.
ModBase	Search...
Genome annotation databases
GeneID	1232739.
GenomeReviews	Gene locus R01095 in contig AL591688_GR.
KEGG	sme:SMc02599.
NMPDR	fig|266834.1.peg.2389.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q92R32.
OMA	Q92R32. AGCHYNN.
Enzyme and pathway databases
BioCyc	SMEL266834:SMC02599-MON.
BRENDA	1.4.3.16. 142.
Family and domain databases
InterPro	IPR003953. FAD_bind2_N. IPR004112. Fum_Rdtase/Succ_DH_flav_C. IPR005288. NadB. [Graphical view]
Pfam	PF00890. FAD_binding_2. 1 hit. PF02910. Succ_DH_flav_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00551. nadB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADB_RHIME

Accession

Primary (citable) accession number: Q92R32

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	December 1, 2001
Last modified:	June 16, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)