Reviewed, UniProtKB/Swiss-Prot Q92QU6 (GLYA1_RHIME)

Names and origin

Protein names

Recommended name:
    Serine hydroxymethyltransferase 1
      Short name=Serine methylase 1
      Short name=SHMT 1
    EC=2.1.2.1

Gene names

Name:	glyA1
Ordered Locus Names:	R01208
ORF Names:	SMc01770

Organism

Rhizobium meliloti (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

382 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	431 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Interconversion of serine and glycine. HAMAP MF_00051
Catalytic activity	5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP MF_00051
Cofactor	Pyridoxal phosphate By similarity.
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_00051
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the SHMT family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-serine metabolic process Inferred from electronic annotation. Source: InterPro glycine metabolic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycine hydroxymethyltransferase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 431	431	Serine hydroxymethyltransferase 1 HAMAP MF_00051		PRO_0000113648
Amino acid modifications
	Modified residue	236	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q92QU6-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 6FDC3CFDDE7704B3	FASTA	431	46,732
	10 20 30 40 50 60 MLSQTNDAFF TRSLADSDPE IFGAIEKELG RQRHEIELIA SENIVSRAVL EAQGSIMTNK 70 80 90 100 110 120 YAEGYPGKRY YGGCQYVDIA EALAIERAKK LFGVNFANVQ PNSGSQMNQA VFLALLQPGD 130 140 150 160 170 180 TFMGLDLNSG GHLTHGSPVN MSGKWFNVVS YGVREDDHLL DMDEVARKAR EQKPKLIIAG 190 200 210 220 230 240 GTAYSRIWDW KRFREIADEV GAWLMVDMAH IAGLVAGGQH PSPFPHCHVA TTTTHKSLRG 250 260 270 280 290 300 PRGGMILTND EEIAKKINSA VFPGLQGGPL MHVIAAKAVA LGEALQPSFK DYAAQVVKNA 310 320 330 340 350 360 RTLAETLKAN GLDIVSGGTD NHLMLVDLRK KNATGKRAEA ALGRAYVTCN KNGIPFDPEK 370 380 390 400 410 420 PFVTSGVRLG APAGTTRGFK EAEFKEVGEL IVEVLDGLKA ANSDEGNAAV EAGVREKVIK 430 LTDRFPMYGY M

References

[1]

"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021." Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F. Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed: 11481430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL591688 Genomic DNA. Translation: CAC45787.1.
RefSeq	NP_385314.1.
3D structure databases
HSSP	HSSP built from PDB template 1DFO based on UniProtKB P00477.
ModBase	Search...
Genome annotation databases
GeneID	1232852.
GenomeReviews	Gene locus R01208 in contig AL591688_GR.
KEGG	sme:SMc01770.
NMPDR	fig|266834.1.peg.2502.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q92QU6.
OMA	HCHVATT.
Enzyme and pathway databases
BioCyc	MetaCyc:MON-8567. SMEL266834:SMC01770-MON.
BRENDA	2.1.2.1. 142.
Family and domain databases
HAMAP	MF_00051. [Tree]
InterPro	IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR001085. Ser_HO-MeTrfase. IPR019798. Ser_HO-MeTrfase_PLP_BS. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11680. Gly_HO-Metrfase. 1 hit.
Pfam	PF00464. SHMT. 1 hit. [Graphical view]
PIRSF	PIRSF000412. SHMT. 1 hit.
PROSITE	PS00096. SHMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLYA1_RHIME

Accession

Primary (citable) accession number: Q92QU6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	December 1, 2001
Last modified:	November 3, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)