Reviewed, UniProtKB/Swiss-Prot Q92Q90 (ISPDF_RHIME)

Names and origin

Protein names

Recommended name:
    Bifunctional enzyme ispD/ispF
Including the following 2 domains:
    1- Recommended name:
            2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
              EC=2.7.7.60
        Alternative name(s):
            4-diphosphocytidyl-2C-methyl-D-erythritol synthase
            MEP cytidylyltransferase
              Short name=MCT
    2- Recommended name:
            2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
                Short name=MECPS
                Short name=MECDP-synthase
              EC=4.6.1.12

Gene names

Name:	ispDF
Ordered Locus Names:	R01456
ORF Names:	SMc01040

Organism

Rhizobium meliloti (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

382 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	410 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme that catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) (ispD), and converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP (ispF) By similarity.
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_01520 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP. HAMAP MF_01520
Cofactor	Divalent metal cations By similarity.
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_01520 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 4/6.
Sequence similarities	In the N-terminal section; belongs to the ispD family. In the C-terminal section; belongs to the ispF family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	Metal-binding
Molecular function	Lyase Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity Inferred from electronic annotation. Source: HAMAP 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 410	410	Bifunctional enzyme ispD/ispF HAMAP MF_01520		PRO_0000075674
Regions
	Region	1 – 243	243	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_01520
	Region	244 – 410	167	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase HAMAP MF_01520
Sites
	Metal binding	250	1	Divalent metal cation By similarity
	Metal binding	252	1	Divalent metal cation By similarity
	Metal binding	284	1	Divalent metal cation By similarity
	Site	21	1	Transition state stabilizer By similarity
	Site	30	1	Transition state stabilizer By similarity
	Site	164	1	Positions MEP for the nucleophilic attack By similarity
	Site	221	1	Positions MEP for the nucleophilic attack By similarity
	Site	276	1	Transition state stabilizer By similarity
	Site	375	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q92Q90-1 [UniParc]. Last modified August 4, 2003. Version 2. Checksum: A53085CF9D8AEA65	FASTA	410	43,580
	10 20 30 40 50 60 MQGEEQFSCG VVIVAAGRGE RAGQSAEGPK QYRTIGDRPV IAHTLDIFAT WPGAGPVVVV 70 80 90 100 110 120 IHPDDEELFA AARKRMAGAL ELTVVHGGAT RQLSVLAGLE AVAATGVEYV MIHDAVRPFF 130 140 150 160 170 180 DHTLLDRCGA ALRDGAQALL PAIAVADTLK RTKAAGLVAE TVPRTDLYAA QTPQCFRLEP 190 200 210 220 230 240 ILSAHRRAAA SGRSDFTDDA SIAEWAGIPV LLVEGAVDNF KLTLRRDLSM ADEKLTRMAI 250 260 270 280 290 300 PDVRTGNGYD VHQLVDGDGV TLCGVFIPHG RKLSGHSDAD VALHALTDAL LATCGAGDIG 310 320 330 340 350 360 DHFPPSDPQW KGAPSRIFLE HAARIVRERG GTVTNADISL IAEAPKVGPH RQQMRENLAA 370 380 390 400 410 ILGISLDRCS IKATTNEKLG FVGRNEGIAA IATATVVYAS RSKPDVDDRY

References

[1]

"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021." Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F. Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed: 11481430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL591688 Genomic DNA. Translation: CAC46035.1. Different initiation.
RefSeq	NP_385562.3.
3D structure databases
HSSP	HSSP built from PDB template 1JY8 based on UniProtKB P36663.
ModBase	Search...
Genome annotation databases
GeneID	1233109.
GenomeReviews	Gene locus R01456 in contig AL591688_GR.
KEGG	sme:SMc01040.
NMPDR	fig|266834.1.peg.2750.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q92Q90.
Enzyme and pathway databases
BioCyc	SMEL266834:SMC01040-MON.
BRENDA	2.7.7.60. 142. 4.6.1.12. 142.
Family and domain databases
HAMAP	MF_01520. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. IPR003526. MECDP_synthase_core. IPR020555. MECDP_synthase_CS. [Graphical view]
Pfam	PF01128. IspD. 1 hit. PF02542. YgbB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00453. ispD. 1 hit. TIGR00151. ispF. 1 hit.
PROSITE	PS01295. ISPD. 1 hit. PS01350. ISPF. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPDF_RHIME

Accession

Primary (citable) accession number: Q92Q90

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2002
Last sequence update:	August 4, 2003
Last modified:	September 22, 2009
This is version 56 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)