Q92PH0 (GLSA_RHIME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutaminase
EC=3.5.1.2

Gene names

Name:	glsA
Ordered Locus Names:	R01786
ORF Names:	SMc00486

Organism

Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

266834 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium › Rhizobium meliloti

Protein attributes

Sequence length	315 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-glutamine + H2O = L-glutamate + NH3. HAMAP-Rule MF_00313
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the glutaminase family.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glutamine metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	glutaminase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 315	315	Glutaminase HAMAP-Rule MF_00313		PRO_0000110621
Sites
	Binding site	70	1	Substrate By similarity
	Binding site	120	1	Substrate By similarity
	Binding site	166	1	Substrate By similarity
	Binding site	173	1	Substrate By similarity
	Binding site	197	1	Substrate By similarity
	Binding site	249	1	Substrate By similarity
	Binding site	267	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q92PH0 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: CB3A62CAB20A2B84	FASTA	315	33,991
	10 20 30 40 50 60 MREPKPQEDL QAIIDDIYRE LTPRLGEGKV ADYIPQLARV DARHFGMAIV TTGGEVYRVG 70 80 90 100 110 120 DAEMPFSIQS ISKVFTLTLA LGKHGENIWN RVGREPSGSA FNSIVQLEHE GGKPRNPFIN 130 140 150 160 170 180 AGAIRISDLI LAGHTPKELI GEIVRFVRYL ADDENIVIDH EVARSETATG YRNIALANFM 190 200 210 220 230 240 RSFGRLDHPV EHVLGVYFHH CALAMTCSQL AKAGLFLAAG GTNPLTGHSV VSRQRARRIN 250 260 270 280 290 300 ALMLTCGHYD GSGDFAYRVG LPGKSGVGGG IMAVAPGKAS IAVWSPGLND YGNSLLGSLA 310 LEMLAARTGW SVFGP

References

[1]

"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021." Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F. Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

[2]

"The composite genome of the legume symbiont Sinorhizobium meliloti." Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F., Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G., Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P., Cowie A. , Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S., Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I., Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S., Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C., Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R., Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H., Wong K., Yeh K.-C., Batut J. Science 293:668-672(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL591688 Genomic DNA. Translation: CAC46365.1.
RefSeq	NP_385892.1. NC_003047.1.
3D structure databases
ProteinModelPortal	Q92PH0.
ModBase	Search...
Protein-protein interaction databases
STRING	266834.SMc00486.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAC46365; CAC46365; SMc00486.
GeneID	1233451.
KEGG	sme:SMc00486.
PATRIC	23632977. VBISinMel96828_3227.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2066.
HOGENOM	HOG000216890.
KO	K01425.
OMA	EPSGNPF.
ProtClustDB	PRK00971.
Enzyme and pathway databases
BioCyc	SMEL266834:GJF6-1834-MONOMER.
Family and domain databases
Gene3D	3.40.710.10. 1 hit.
HAMAP	MF_00313. Glutaminase.
InterPro	IPR012338. Beta-lactam/transpept-like. IPR015868. Glutaminase. [Graphical view]
PANTHER	PTHR12544. PTHR12544. 1 hit.
Pfam	PF04960. Glutaminase. 1 hit. [Graphical view]
SUPFAM	SSF56601. PBP_transp_fold. 1 hit.
TIGRFAMs	TIGR03814. Gln_ase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLSA_RHIME

Accession

Primary (citable) accession number: Q92PH0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2002
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program