Reviewed, UniProtKB/Swiss-Prot Q92NH8 (TPIS2_RHIME)

Names and origin

Protein names

Recommended name:
    Triosephosphate isomerase 2
      Short name=TIM 2
    EC=5.3.1.1
Alternative name(s):
    Triose-phosphate isomerase 2

Gene names

Name:	tpiA2
Ordered Locus Names:	R02226
ORF Names:	SMc01614

Organism

Rhizobium meliloti (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

382 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	255 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate = glycerone phosphate. HAMAP MF_00147
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00147 Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1. HAMAP MF_00147
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the triosephosphate isomerase family.

Ontologies

Keywords
Biological process	Gluconeogenesis Glycolysis Pentose shunt
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: HAMAP glycolysis Inferred from electronic annotation. Source: HAMAP pentose-phosphate shunt Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triose-phosphate isomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 255	255	Triosephosphate isomerase 2 HAMAP MF_00147		PRO_0000090277
Sites
	Active site	98	1	Electrophile By similarity
	Active site	171	1	Proton acceptor By similarity
	Binding site	13	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q92NH8-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 2696EF571B14686E	FASTA	255	27,623
	10 20 30 40 50 60 MSKSGLWVGT SWKMNKTLAE AMVFADGLAA ADDARDQRIQ RFVIPPFTAA RQVKERLKAT 70 80 90 100 110 120 SVKVGAQNMH WDDAGAWTGE ISPVMLSDCD LDIVELGHSE RREHFGETDR TVGLKTAAAV 130 140 150 160 170 180 KHGLVPLICI GETLAQREAG EADTVLKRQV EGAFAFLEGA ARKAPVLLAY EPVWAIGVNG 190 200 210 220 230 240 IPATADYADA RHRLIGEVAE RALGVKVPVL YGGSVNPQNC EELILQPHID GLFIGRSAWD 250 VGGYLDILQR VARAI

References

[1]

"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021." Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F. Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed: 11481430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL591688 Genomic DNA. Translation: CAC46805.1.
RefSeq	NP_386332.1.
3D structure databases
HSSP	HSSP built from PDB template 2BTM based on UniProtKB P00943.
ModBase	Search...
Genome annotation databases
GeneID	1233892.
GenomeReviews	Gene locus R02226 in contig AL591688_GR.
KEGG	sme:SMc01614.
NMPDR	fig|266834.1.peg.3520.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q92NH8.
OMA	EHFGETD.
Enzyme and pathway databases
BioCyc	SMEL266834:SMC01614-MON.
BRENDA	5.3.1.1. 142.
Family and domain databases
HAMAP	MF_00147. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR000652. Triosephosphate_isomerase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR21139. Triophos_ismrse. 1 hit.
Pfam	PF00121. TIM. 1 hit. [Graphical view]
ProDom	PD001005. Triophos_ismrse. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00419. tim. 1 hit.
PROSITE	PS00171. TIM_1. 1 hit. PS51440. TIM_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TPIS2_RHIME

Accession

Primary (citable) accession number: Q92NH8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	December 1, 2001
Last modified:	November 3, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)