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Q92M14 (GLGB_RHIME) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:R02844
ORF Names:SMc03922
OrganismRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) [Complete proteome] [HAMAP]
Taxonomic identifier266834 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium

Protein attributes

Sequence length736 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7367361,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000188735

Sites

Active site4191Nucleophile By similarity
Active site4721Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q92M14 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 24F194CC9A19C363

FASTA73683,388
        10         20         30         40         50         60 
MTVPPGKDPA PAPPGMLPAP EIAAILSGTH SNPFAVLGVH AAGKTYVARC FVPGAETVIA 

        70         80         90        100        110        120 
ETLSGKELGT LERRDEAGFF EGPVALRKEQ PIRYRARNDG GEWTVIDPYS FGPVLGPMDD 

       130        140        150        160        170        180 
YYIREGSHLR LFDKMGAHPI RHDGAEGFHF AVWAPNARRV SVVGSFNDWD GRRHVMRLRV 

       190        200        210        220        230        240 
DTGIWEIFIP GVPVGTPYKY EIVDSNGTLL PLKADPFARR SELRPDTASM TADEIAQNWE 

       250        260        270        280        290        300 
DEAHRRHWAE IDPRRQPISI YEVHAASWQR RDNGDMLTWD ELAERLIPYC VDMGFTHIEF 

       310        320        330        340        350        360 
LPISEYPYDP SWGYQTTGLY APTARFGEPE GFARFVNGCH KVGIGVILDW VPAHFPTDEH 

       370        380        390        400        410        420 
GLRWFDGTAL YEHADPRQGF HPDWNTAIYN FGRQEVVAFL VNNALYWAEK FHVDGLRVDA 

       430        440        450        460        470        480 
VASMLYLDYS RKHGEWVPNE YGGNENLEAV RFLQTMNTRI YGIHQGVLTI AEESTSWPKV 

       490        500        510        520        530        540 
SHPVHAGGLG FGFKWNMGFM HDTLQYLSRE PVHRKFHHND MTFGLLYAFS ENFVLPLSHD 

       550        560        570        580        590        600 
EVVHGKGSLI AKMAGDDWQK FANLRAYYGF MWGYPGKKLL FMGQEFAQWR EWSEDRGLDW 

       610        620        630        640        650        660 
NLLEYNLHEG MRRLVRDLNG TYRSKPALHA RDCEGDGFEW LIADDRENSV FAWLRKAPGE 

       670        680        690        700        710        720 
KLVAVVTNFT PVYREHYDIP LPVAGRWKEV LNTDAEIYGG SGKGNGGAVH AEKRANGETI 

       730 
ATITLPPLAT LMLEQD 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL591688 Genomic DNA. Translation: CAC47423.1.
RefSeqNP_386950.1. NC_003047.1.

3D structure databases

ProteinModelPortalQ92M14.
SMRQ92M14. Positions 127-736.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING266834.SMc03922.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAC47423; CAC47423; SMc03922.
GeneID1234521.
KEGGsme:SMc03922.
PATRIC23635268. VBISinMel96828_4363.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAISEGRHE.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycSMEL266834:GJF6-2913-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_RHIME
AccessionPrimary (citable) accession number: Q92M14
Entry history
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: December 1, 2001
Last modified: June 11, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries