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Q92M14

- GLGB_RHIME

UniProt

Q92M14 - GLGB_RHIME

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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene
glgB, R02844, SMc03922
Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei419 – 4191Nucleophile By similarity
Active sitei472 – 4721Proton donor By similarity

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. glycogen biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BioCyciSMEL266834:GJF6-2913-MONOMER.
UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgB (EC:2.4.1.18)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name:
BE
Gene namesi
Name:glgB
Ordered Locus Names:R02844
ORF Names:SMc03922
OrganismiRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Taxonomic identifieri266834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
ProteomesiUP000001976: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7367361,4-alpha-glucan branching enzyme GlgBUniRule annotationPRO_0000188735Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi266834.SMc03922.

Structurei

3D structure databases

ProteinModelPortaliQ92M14.
SMRiQ92M14. Positions 127-736.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiISEGRHE.
OrthoDBiEOG6JX7GT.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

Q92M14-1 [UniParc]FASTAAdd to Basket

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MTVPPGKDPA PAPPGMLPAP EIAAILSGTH SNPFAVLGVH AAGKTYVARC    50
FVPGAETVIA ETLSGKELGT LERRDEAGFF EGPVALRKEQ PIRYRARNDG 100
GEWTVIDPYS FGPVLGPMDD YYIREGSHLR LFDKMGAHPI RHDGAEGFHF 150
AVWAPNARRV SVVGSFNDWD GRRHVMRLRV DTGIWEIFIP GVPVGTPYKY 200
EIVDSNGTLL PLKADPFARR SELRPDTASM TADEIAQNWE DEAHRRHWAE 250
IDPRRQPISI YEVHAASWQR RDNGDMLTWD ELAERLIPYC VDMGFTHIEF 300
LPISEYPYDP SWGYQTTGLY APTARFGEPE GFARFVNGCH KVGIGVILDW 350
VPAHFPTDEH GLRWFDGTAL YEHADPRQGF HPDWNTAIYN FGRQEVVAFL 400
VNNALYWAEK FHVDGLRVDA VASMLYLDYS RKHGEWVPNE YGGNENLEAV 450
RFLQTMNTRI YGIHQGVLTI AEESTSWPKV SHPVHAGGLG FGFKWNMGFM 500
HDTLQYLSRE PVHRKFHHND MTFGLLYAFS ENFVLPLSHD EVVHGKGSLI 550
AKMAGDDWQK FANLRAYYGF MWGYPGKKLL FMGQEFAQWR EWSEDRGLDW 600
NLLEYNLHEG MRRLVRDLNG TYRSKPALHA RDCEGDGFEW LIADDRENSV 650
FAWLRKAPGE KLVAVVTNFT PVYREHYDIP LPVAGRWKEV LNTDAEIYGG 700
SGKGNGGAVH AEKRANGETI ATITLPPLAT LMLEQD 736
Length:736
Mass (Da):83,388
Last modified:December 1, 2001 - v1
Checksum:i24F194CC9A19C363
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL591688 Genomic DNA. Translation: CAC47423.1.
RefSeqiNP_386950.1. NC_003047.1.

Genome annotation databases

EnsemblBacteriaiCAC47423; CAC47423; SMc03922.
GeneIDi1234521.
KEGGisme:SMc03922.
PATRICi23635268. VBISinMel96828_4363.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL591688 Genomic DNA. Translation: CAC47423.1 .
RefSeqi NP_386950.1. NC_003047.1.

3D structure databases

ProteinModelPortali Q92M14.
SMRi Q92M14. Positions 127-736.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 266834.SMc03922.

Protein family/group databases

CAZyi CBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAC47423 ; CAC47423 ; SMc03922 .
GeneIDi 1234521.
KEGGi sme:SMc03922.
PATRICi 23635268. VBISinMel96828_4363.

Phylogenomic databases

eggNOGi COG0296.
HOGENOMi HOG000283037.
KOi K00700.
OMAi ISEGRHE.
OrthoDBi EOG6JX7GT.

Enzyme and pathway databases

UniPathwayi UPA00164 .
BioCyci SMEL266834:GJF6-2913-MONOMER.

Family and domain databases

Gene3Di 2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_00685. GlgB.
InterProi IPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view ]
PIRSFi PIRSF000463. GlgB. 1 hit.
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsi TIGR01515. branching_enzym. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1021.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1021.

Entry informationi

Entry nameiGLGB_RHIME
AccessioniPrimary (citable) accession number: Q92M14
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: December 1, 2001
Last modified: June 11, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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