Reviewed, UniProtKB/Swiss-Prot Q92LF7 (PROB2_RHIME)

Names and origin

Protein names

Recommended name:
    Glutamate 5-kinase 2
    EC=2.7.2.11
Alternative name(s):
    Gamma-glutamyl kinase 2
      Short name=GK 2

Gene names

Name:	proB2
Ordered Locus Names:	R03106
ORF Names:	SMc03252

Organism

Rhizobium meliloti (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

382 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	269 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline. HAMAP MF_00456
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP MF_00456
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glutamate 5-kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate 5-kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 269	269	Glutamate 5-kinase 2 HAMAP MF_00456		PRO_0000109716

Sequences

	Sequence		Length	Mass (Da)
	Q92LF7-1 [UniParc]. Last modified July 26, 2002. Version 2. Checksum: 9DE5148CA9ECB292	FASTA	269	28,953
	10 20 30 40 50 60 MEDKFDLSSA RLVVVKIGST LVLDRETSGI RSSWLESLTE DVSRLLTRGQ QVVLVSSGAV 70 80 90 100 110 120 AIGSTIVDRL ATYSQVSHKQ AAAALGQVQL THAYSESLKR HGLQVAQLLM GRGDLVDPAH 130 140 150 160 170 180 RLNTRAVLLR LIDLGAVPLV NENDTTATCG TRVGDNDRLA AWIAEIINAD LLILLSNVDG 190 200 210 220 230 240 LFMKDPRNNP LTPMLTEVES ITREIEAMAT QSVDPYSSGG MISKIEAGKI AMNAGCRMII 250 260 ANGTRSHPLY AIESGGPSTH FIPVARDRV

References

[1]

"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021." Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F. Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed: 11481430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL591688 Genomic DNA. Translation: CAC47685.1. Different initiation.
RefSeq	NP_387212.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1234791.
GenomeReviews	Gene locus R03106 in contig AL591688_GR.
KEGG	sme:SMc03252.
NMPDR	fig|266834.1.peg.4400.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q92LF7.
Enzyme and pathway databases
BioCyc	SMEL266834:SMC03252-MON.
BRENDA	2.7.2.11. 142.
Family and domain databases
HAMAP	MF_00456. [Tree]
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu_5kinase. IPR011529. Glu_5kinase_bact. IPR019797. Glutamate_5-kinase_CS. IPR005715. ProB. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
TIGRFAMs	TIGR01027. proB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB2_RHIME

Accession

Primary (citable) accession number: Q92LF7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 26, 2002
Last sequence update:	July 26, 2002
Last modified:	June 16, 2009
This is version 45 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)