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Q92LF7 (PROB2_RHIME) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate 5-kinase 2

EC=2.7.2.11
Alternative name(s):
Gamma-glutamyl kinase 2
Short name=GK 2
Gene names
Name:proB2
Ordered Locus Names:R03106
ORF Names:SMc03252
OrganismRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) [Complete proteome] [HAMAP]
Taxonomic identifier266834 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium

Protein attributes

Sequence length269 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline By similarity. HAMAP-Rule MF_00456

Catalytic activity

ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP-Rule MF_00456

Pathway

Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP-Rule MF_00456

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00456.

Sequence similarities

Belongs to the glutamate 5-kinase family.

Sequence caution

The sequence CAC47685.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Proline biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processL-proline biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutamate 5-kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 269269Glutamate 5-kinase 2 HAMAP-Rule MF_00456
PRO_0000109716

Regions

Nucleotide binding218 – 2247ATP By similarity

Sites

Binding site161ATP By similarity
Binding site571Substrate By similarity
Binding site1441Substrate By similarity
Binding site1561Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q92LF7 [UniParc].

Last modified July 26, 2002. Version 2.
Checksum: 9DE5148CA9ECB292

FASTA26928,953
        10         20         30         40         50         60 
MEDKFDLSSA RLVVVKIGST LVLDRETSGI RSSWLESLTE DVSRLLTRGQ QVVLVSSGAV 

        70         80         90        100        110        120 
AIGSTIVDRL ATYSQVSHKQ AAAALGQVQL THAYSESLKR HGLQVAQLLM GRGDLVDPAH 

       130        140        150        160        170        180 
RLNTRAVLLR LIDLGAVPLV NENDTTATCG TRVGDNDRLA AWIAEIINAD LLILLSNVDG 

       190        200        210        220        230        240 
LFMKDPRNNP LTPMLTEVES ITREIEAMAT QSVDPYSSGG MISKIEAGKI AMNAGCRMII 

       250        260 
ANGTRSHPLY AIESGGPSTH FIPVARDRV 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL591688 Genomic DNA. Translation: CAC47685.1. Different initiation.
RefSeqNP_387212.1. NC_003047.1.

3D structure databases

ProteinModelPortalQ92LF7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING266834.SMc03252.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAC47685; CAC47685; SMc03252.
GeneID1234791.
KEGGsme:SMc03252.
PATRIC23635840. VBISinMel96828_4643.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0263.
HOGENOMHOG000246368.
KOK00931.
OrthoDBEOG6PGK7G.

Enzyme and pathway databases

BioCycSMEL266834:GJF6-3185-MONOMER.
UniPathwayUPA00098; UER00359.

Family and domain databases

Gene3D3.40.1160.10. 1 hit.
HAMAPMF_00456. ProB.
InterProIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
[Graphical view]
PfamPF00696. AA_kinase. 1 hit.
[Graphical view]
PIRSFPIRSF000729. GK. 1 hit.
PRINTSPR00474. GLU5KINASE.
SUPFAMSSF53633. SSF53633. 1 hit.
TIGRFAMsTIGR01027. proB. 1 hit.
PROSITEPS00902. GLUTAMATE_5_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePROB2_RHIME
AccessionPrimary (citable) accession number: Q92LF7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 26, 2002
Last sequence update: July 26, 2002
Last modified: May 14, 2014
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways