Reviewed, UniProtKB/Swiss-Prot Q92LB3 (PROB1_RHIME)

Names and origin

Protein names

Recommended name:
    Glutamate 5-kinase 1
    EC=2.7.2.11
Alternative name(s):
    Gamma-glutamyl kinase 1
      Short name=GK 1

Gene names

Name:	proB1
Ordered Locus Names:	R03160
ORF Names:	SMc03776

Organism

Rhizobium meliloti (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

382 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline. HAMAP MF_00456
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP MF_00456
Subcellular location	Cytoplasm By similarity HAMAP MF_00456.
Sequence similarities	Belongs to the glutamate 5-kinase family. Contains 1 PUA domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro glutamate 5-kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 393	393	Glutamate 5-kinase 1 HAMAP MF_00456		PRO_0000109715
Regions
	Domain	282 – 359	78	PUA

Sequences

	Sequence		Length	Mass (Da)
	Q92LB3-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 2424B9FEDDEF9C22	FASTA	393	40,869
	10 20 30 40 50 60 MTKARKALEN YRRVVIKIGS ALLVDRRTGL KKSWLDALCA DIAALRAKGV EVLVVSSGAI 70 80 90 100 110 120 ALGRTVLDLP AGALKLEESQ AAAAVGQIVL ARAWSESLST HAIVAGQILL TLGDTEERRR 130 140 150 160 170 180 YLNARATIGQ LLKLGSVPII NENDTVATTE IRYGDNDRLA ARVATMVGAD LLVLLSDIDG 190 200 210 220 230 240 LYTAPPHLDP NARFLETVAE ITPEIEAMAG GAASELSRGG MRTKIDAGKI ATTAGCAMII 250 260 270 280 290 300 ASGKPDHPLA AIEAGARSSW FAPSGSPVTA RKTWIAGQLL PAGSLSIDAG AETALRSGKS 310 320 330 340 350 360 LLPAGVRQVT GSFSRGDTIA IIGASGREIA RGLAGYDADE ARQIAGKKSA EIAAILGYAG 370 380 390 RTAMVHRDDL VMTAPSGARL VEESDEGKGK LHA

References

[1]

"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021." Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F. Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed: 11481430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL591688 Genomic DNA. Translation: CAC47739.1.
RefSeq	NP_387266.1.
3D structure databases
SMR	Q92LB3. Positions 10-372.
ModBase	Search...
Genome annotation databases
GeneID	1234853.
GenomeReviews	Gene locus R03160 in contig AL591688_GR.
KEGG	sme:SMc03776.
NMPDR	fig|266834.1.peg.4454.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG507643.
OMA	TDVDRLY.
Enzyme and pathway databases
BioCyc	SMEL266834:SMC03776-MONOMER.
BRENDA	2.7.2.11. 142.
Family and domain databases
HAMAP	MF_00456. ProB. [Tree]
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu_5kinase. IPR011529. Glu_5kinase_bact. IPR019797. Glutamate_5-kinase_CS. IPR005715. ProB. IPR002478. PUA. IPR015947. PUA-like. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF01472. PUA. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SMART	SM00359. PUA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01027. proB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. PS50890. PUA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB1_RHIME

Accession

Primary (citable) accession number: Q92LB3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 26, 2002
Last sequence update:	December 1, 2001
Last modified:	February 9, 2010
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)