Reviewed, UniProtKB/Swiss-Prot Q92LA6 (DNE21_RHIME)

Names and origin

Protein names

Recommended name:
    Error-prone DNA polymerase 1
    EC=2.7.7.7

Gene names

Name:	dnaE2-1
Ordered Locus Names:	R03171
ORF Names:	SMc03788

Organism

Rhizobium meliloti (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

382 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	1116 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity. HAMAP MF_01902
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	Cytoplasm Probable HAMAP MF_01902.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1116	1116	Error-prone DNA polymerase 1 HAMAP MF_01902		PRO_0000103395

Sequences

	Sequence		Length	Mass (Da)
	Q92LA6-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: C8D6339601CEB405	FASTA	1,116	124,842
	10 20 30 40 50 60 MSADAVFCEL GARTNFSFLE GAAPAEEMVV FAKKAGLAGL GIADRNSVAG VVRAHAKAKV 70 80 90 100 110 120 EGYPFQPGAR LVFADGTPDI LAYPKNRRGW GHLCRLLSAG NLRSKKGDCT LHLADLLEWQ 130 140 150 160 170 180 EELLLIVMQG EGRPEPESLE VLLGTLKEHA GNRLYLGLAP HYDGFDRHDF AVLAAIARKA 190 200 210 220 230 240 GIGLLATNDA LYHDPHYRPL ADVVTSIREH VPIAGAGFLL QKNAERHLKG PREMARLFSD 250 260 270 280 290 300 YPEAIANTRK FFRELAFSLD ELSHQYPDEN ADGETPAESL RRLVAEGAAE RYPEGVPEKV 310 320 330 340 350 360 MRQIDYELEL IHDKKYEPYF LTVHKLVKFA RSVNILCQGR GSAANSSVCF CLGITDVDPQ 370 380 390 400 410 420 KFTLLFDRFL SKDRDEPPDI DVDFEHERRE EVIQYIYRTY GKEHAGLTAA VISYRSRSAG 430 440 450 460 470 480 REVAKAFGLS EDVQSALVSS IWGWGTSPFT EEQAKGAGLD AADPLTRRVL AYASLLMNFP 490 500 510 520 530 540 RHLSQHVGGF VITRDRLDEV VPIMNTAMPD RYMIEWDKDD LDELKILKVD VLALGMLTCL 550 560 570 580 590 600 AKGFKLLEAH YGEPITLAEI YQDHRDAVYD MICRADTVGV FQIESRAQMS MLPRLQPREM 610 620 630 640 650 660 YDLVIEVAIV RPGPIQGNMV HPYLKRREAQ RRGEAVVYPS PELKAVLERT LGVPLFQEQA 670 680 690 700 710 720 MQIAITAAGF SPSEADRLRR AMATFKRTGT IHTFERKMVE GMVANDYERE FAERCFNQIK 730 740 750 760 770 780 GFGEYGFPES HAASFASLVY ASAWLKTYYP DIFCAALLNA QPMGFYAPAQ LVRDAREHGV 790 800 810 820 830 840 RMLPVDINHS DWDALLEGEG AFDKNAVHPR HASMREVIKT RKAVRLGFRL VKGLKQTDMK 850 860 870 880 890 900 ALVARRGEGY RSVHDLWLRS GLSRSVLERL ADADAFRSIG LDRRAALWAV KALDEQSAVE 910 920 930 940 950 960 RLPLFEGAGS DDLQIEPKVA LPDMPAGEQV IHDYRTLTLS LKAHPVSFMR EDFSRRGILR 970 980 990 1000 1010 1020 SRDLAATATG RWVTVAGLVL VRQRPGSANG VIFMTIEDET GIANIIVWEK TFQKYRRQVM 1030 1040 1050 1060 1070 1080 GSRLVKVRGR LQNQSGVIHV VADHLEDITP MLGLLRREAR RFGVNDRADG ALRPSADARE 1090 1100 1110 KKKLRQLRLG LPARAAPEGE AAAQVAEVMP KGRNFH

References

[1]

"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021." Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F. Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed: 11481430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL591688 Genomic DNA. Translation: CAC47750.1.
RefSeq	NP_387277.1.
3D structure databases
SMR	Q92LA6. Positions 1-892.
ModBase	Search...
Genome annotation databases
GeneID	1234864.
GenomeReviews	Gene locus R03171 in contig AL591688_GR.
KEGG	sme:SMc03788.
NMPDR	fig|266834.1.peg.4465.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734490.
OMA	TIFATIE.
Enzyme and pathway databases
BioCyc	SMEL266834:SMC03788-MONOMER.
BRENDA	2.7.7.7. 142.
Family and domain databases
HAMAP	MF_01902. DNApol_error_prone. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR004365. NA_bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. Pol/His_phosphatase_N. IPR004805. PolC_alpha. [Graphical view]
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00594. polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNE21_RHIME

Accession

Primary (citable) accession number: Q92LA6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	December 1, 2001
Last modified:	February 9, 2010
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)