Q92KX2 (ACSA2_RHIME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetyl-coenzyme A synthetase 2
EC=6.2.1.1

Alternative name(s):
Acetate--CoA ligase 2
Acyl-activating enzyme 2

Gene names

Name:	acsA2
Ordered Locus Names:	R03327
ORF Names:	SMc04093

Organism

Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

266834 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	649 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA. HAMAP MF_01123
Post-translational modification	Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme By similarity. HAMAP MF_01123
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	AMP binding Inferred from electronic annotation. Source: InterPro ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetate-CoA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 649	649	Acetyl-coenzyme A synthetase 2 HAMAP MF_01123		PRO_0000208382
Sites
	Active site	517	1	By similarity
Amino acid modifications
	Modified residue	609	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q92KX2 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: ACC3ED95B87BAC92	FASTA	649	72,022
	10 20 30 40 50 60 MDVKTYPVLE AAKNRTLLDN ATYLEWYRES VADPEKFWGE HGKRIEWFEP YTKVKNTSFE 70 80 90 100 110 120 GDVSIKWFED GLTNVSYNCI DRHLKTHGEK TAIIWEGDNP YLDKKITYNE LYDKVCRLAN 130 140 150 160 170 180 VLKEQGVKKG DRVTIYMPMI PEAAYAMLAC ARIGAIHSVV FGGFSPEALA GRIVDCESTF 190 200 210 220 230 240 VITCDEGVRG GKPVALKENT DTAIDIAARQ HVTVSKVLVV RRTGGKVGWA PGRDLWYHQE 250 260 270 280 290 300 TAAAEPHCPP EKMNAEDPLF ILYTSGSTGK PKGVLHTTGG YLVYASMTHQ YVFDYQDGDI 310 320 330 340 350 360 YWCTADVGWV TGHSYIVYGP LANAATTLMF EGVPNFPDAG RFWEVVDKHK VNIFYTAPTA 370 380 390 400 410 420 IRSLMGAGDD FVKRSSRSSL RLLGTVGEPI NPEAWEWYYH VVGDERCPVV DTWWQTETGG 430 440 450 460 470 480 ILITPLPGAT DLKPGSATRP FFGVQPQIVD SDGKVVDGAA DGNLCITDSW PGQMRTVYGD 490 500 510 520 530 540 HERFIQTYFS TYKGKYFTGD GCRRDEDGYY WITGRVDDVL NVSGHRLGTA EVESALVSHN 550 560 570 580 590 600 LVSEAAVVGY PHPIKGQGIY CYVSLMAGEV GDDELRQALV KHVRSEIGPI ATPDKIQFAP 610 620 630 640 GLPKTRSGKI MRRILRKIAE DDFGSLGDTS TLADPGVVDD LIANRQNRA

References

[1]

"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021." Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F. Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed: 11481430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

[2]

"The composite genome of the legume symbiont Sinorhizobium meliloti." Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F., Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G., Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P., Cowie A. , Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S., Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I., Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S., Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C., Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R., Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H., Wong K., Yeh K.-C., Batut J. Science 293:668-672(2001) [PubMed: 11474104] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL591688 Genomic DNA. Translation: CAC47906.1.
RefSeq	NP_387433.1. NC_003047.1.
3D structure databases
ProteinModelPortal	Q92KX2.
SMR	Q92KX2. Positions 14-646.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1235021.
GenomeReviews	Gene locus R03327 in contig AL591688_GR.
KEGG	sme:SMc04093.
NMPDR	fig|266834.1.peg.4621.
PATRIC	23636332. VBISinMel96828_4888.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG547964.
OMA	KGKPYML.
ProtClustDB	PRK00174.
Enzyme and pathway databases
BioCyc	SMEL266834:SMC04093-MONOMER.
Family and domain databases
HAMAP	MF_01123. Ac_CoA_synth. [Tree]
InterPro	IPR011904. Ac_CoA_lig. IPR024597. Acyl-CoA_synth_DUF3448. IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
KO	K01895.
PANTHER	PTHR24095:SF42. PTHR24095:SF42. 1 hit.
Pfam	PF00501. AMP-binding. 1 hit. PF11930. DUF3448. 1 hit. [Graphical view]
TIGRFAMs	TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACSA2_RHIME

Accession

Primary (citable) accession number: Q92KX2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2003
Last sequence update:	December 1, 2001
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program