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Q92KW8 (SYL_RHIME) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:R03331
ORF Names:SMc02804
OrganismRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) [Complete proteome] [HAMAP]
Taxonomic identifier266834 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium

Protein attributes

Sequence length876 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 876876Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152071

Regions

Motif43 – 5311"HIGH" region HAMAP-Rule MF_00049
Motif632 – 6365"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6351ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q92KW8 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: B483BEDFEA63C084

FASTA87698,189
        10         20         30         40         50         60 
MATERYNPRD AEPRWQHQWE AGKVFETKND DPRDKYYVLE MFPYPSGRIH MGHVRNYTMG 

        70         80         90        100        110        120 
DVVARYKRAR GFNVLHPMGW DAFGMPAENA AMERGVHPAS WTYQNIASMK AQLKVMGLSL 

       130        140        150        160        170        180 
DWSREFATCD PEYYQRQQHL FLDFLEKGLV YRKQSKVNWD PVDNTVLANE QVIEGRGWRS 

       190        200        210        220        230        240 
GALVEQRELT QWFFRITDFS QDLLDALDTL DEWPEKVRLM QKNWIGRSEG LSVRWELDPA 

       250        260        270        280        290        300 
TVPGEEKEVT VYTTRPDTLF GASFLAISAD HPLARDAAAK NAEIEAFCEE CRRAGTSLAA 

       310        320        330        340        350        360 
LETAEKKGID TGIRARHPFD PNWELPVYVA NFVLMDYGTG AIFGCPSGDQ RDLDFARKYD 

       370        380        390        400        410        420 
LPVVPVVMPK DADPQTFTIG DEAYDGDGVM INSRFLDGLS AEEAFETVAS RLENDLLNGT 

       430        440        450        460        470        480 
PRAERKVNFR LRDWGISRQR YWGCPIPVIH CDDCGVVPVP KADLPVTLPP DVTFDKPGNP 

       490        500        510        520        530        540 
LDRHPTWRHV ACPQCGKDAR RETDTMDTFV DSSWYFTRFT APWEDNPTDP KAANHWLPVD 

       550        560        570        580        590        600 
QYIGGIEHAI LHLLYSRFFT RAMKATGHVA LDEPFKGLFT QGMVVHETYS RGEGAQREWI 

       610        620        630        640        650        660 
TPAEIRVEEV DGQRRAVHIE TGEEVAIGSI EKMSKSKKNV VDPDDIIGSY GADTARFFVL 

       670        680        690        700        710        720 
SDSPPDRDVI WSEAGVEGAH RFVQRVWRLL TEAAERLRAV DAAPASGGEG LAVSQAAHRT 

       730        740        750        760        770        780 
LKAVEADYDK LAFNKAVARI YELVNAMAAP LTQVASGKAD NALTAAVKDA AAILINLIAP 

       790        800        810        820        830        840 
MMPHLAEECW REIGGKGLIA ERPWPKFDPA LIVENEITLP VQINGKKRAD LTIARDADQS 

       850        860        870 
AIESAVLALD AVKAALNGGR PKKIIVVPQR IVNVVV 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL591688 Genomic DNA. Translation: CAC47910.1.
RefSeqNP_387437.1. NC_003047.1.

3D structure databases

ProteinModelPortalQ92KW8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING266834.SMc02804.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAC47910; CAC47910; SMc02804.
GeneID1235025.
KEGGsme:SMc02804.
PATRIC23636340. VBISinMel96828_4892.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycSMEL266834:GJF6-3422-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_RHIME
AccessionPrimary (citable) accession number: Q92KW8
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: December 1, 2001
Last modified: April 16, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries