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Protein

Chaperone protein ClpB

Gene

clpB

Organism
Rickettsia conorii (strain ATCC VR-613 / Malish 7)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase activity; ATP hydrolysis unfolds the denatured protein aggregates, which probably helps expose new hydrophobic binding sites on the surface of ClpB-bound aggregates, contributing to the solubilization and refolding of denatured protein aggregates by DnaK (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi207 – 214ATP 1By similarity8
Nucleotide bindingi604 – 611ATP 2By similarity8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperone
Biological processStress response
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRCON272944:G1FZG-93-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein ClpB
Gene namesi
Name:clpB
Ordered Locus Names:RC0059
OrganismiRickettsia conorii (strain ATCC VR-613 / Malish 7)
Taxonomic identifieri272944 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
Proteomesi

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001911691 – 857Chaperone protein ClpBAdd BLAST857

Proteomic databases

PRIDEiQ92JK8

Interactioni

Subunit structurei

Homohexamer. The oligomerization is ATP-dependent (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ92JK8
SMRiQ92JK8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 144N-terminalBy similarityAdd BLAST144
Regioni160 – 341NBD1By similarityAdd BLAST182
Regioni342 – 544LinkerBy similarityAdd BLAST203
Regioni554 – 764NBD2By similarityAdd BLAST211
Regioni765 – 857C-terminalBy similarityAdd BLAST93

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili392 – 523By similarityAdd BLAST132

Domaini

The N-terminal domain probably functions as a substrate-discriminating domain, recruiting aggregated proteins to the ClpB hexamer and/or stabilizing bound proteins. The NBD2 domain is responsible for oligomerization, whereas the NBD1 domain stabilizes the hexamer probably in an ATP-dependent manner. The movement of the coiled-coil domain is essential for ClpB ability to rescue proteins from an aggregated state, probably by pulling apart large aggregated proteins, which are bound between the coiled-coils motifs of adjacent ClpB subunits in the functional hexamer (By similarity).By similarity

Sequence similaritiesi

Belongs to the ClpA/ClpB family.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

HOGENOMiHOG000218211
KOiK03695
OMAiTQVIRML
OrthoDBiPOG091H019M

Family and domain databases

Gene3Di1.10.1780.10, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR003959 ATPase_AAA_core
IPR017730 Chaperonin_ClpB
IPR019489 Clp_ATPase_C
IPR004176 Clp_N
IPR036628 Clp_N_dom_sf
IPR001270 ClpA/B
IPR018368 ClpA/B_CS1
IPR028299 ClpA/B_CS2
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00004 AAA, 1 hit
PF07724 AAA_2, 1 hit
PF02861 Clp_N, 2 hits
PF10431 ClpB_D2-small, 1 hit
PRINTSiPR00300 CLPPROTEASEA
SMARTiView protein in SMART
SM00382 AAA, 2 hits
SM01086 ClpB_D2-small, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
SSF81923 SSF81923, 1 hit
TIGRFAMsiTIGR03346 chaperone_ClpB, 1 hit
PROSITEiView protein in PROSITE
PS00870 CLPAB_1, 1 hit
PS00871 CLPAB_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q92JK8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIDKFTAHA KSVIASSQLL AAKNDHQQIL PLHLLSSLLS EETGIIQTLI
60 70 80 90 100
NNTGGNINLL KDQVQLELNK IPKVQVEGGG QVYSSAEALK VLEKASSIAK
110 120 130 140 150
DNGDSFVTIE RIFEALTYDN TIAGKILTNN GINNKKIATA ILQFRKGKKA
160 170 180 190 200
DTESAENSYD ALKKYGRDVT ELAESGKLDP IIGRDEEIRR TVQVLSRRMK
210 220 230 240 250
NNPVLIGEPG VGKTAIIEGL AQRIFSKDVP ESLINCRIIE LDMGALIAGA
260 270 280 290 300
KYRGEFEERL KAVLSEIKES SGEIILFIDE LHLLVGTGKT DGAMDASNLL
310 320 330 340 350
KPMLARGELH CIGATTLDEY RKYIEKDAAL ARRFQPVYVS EPTVEDTISI
360 370 380 390 400
LRGIKEKYEL HHAVRISDSA IVAAATLSNR YITDRYLPDK AIDLIDEACS
410 420 430 440 450
RMKIELSSKP EELDELDRRI IQIKIELAAL KKENDEHSKK KIEHLTTALE
460 470 480 490 500
KLESQSYDMG AKWQAEKSKL QQTQKLKEEL DRSRNELERA ERDANLAKAS
510 520 530 540 550
ELKYGIIPEI MKKLQEAESM DNKGLLKEIV SESDIASIIS RITGIPIDTM
560 570 580 590 600
LSSERERLLV MEQKLRESVI GQDKAIKGVS DAVRRSRAGI QDINRPLGSF
610 620 630 640 650
LFLGPTGVGK TELTKALAGF LFDDRNAILR IDMSEYMEKH AISRLIGAPP
660 670 680 690 700
GYIGYDQGGV LTEAVRRRPY QVILFDEVEK AHPDIFNIML QILDEGRLTD
710 720 730 740 750
SQGITVDFKN TIIVLTSNLG AEILVNQKED EDTYKVKDEV MEYVKAVFKP
760 770 780 790 800
EFLNRLDEII LFHRLNRNNI HDIVKIQLES LKKILLAQNI ILEFDESALN
810 820 830 840 850
YLAEKGYDPS FGARPLKRLI QREIQNNLAK MILAGEISSG KTVKINSKDK

ELKVTMS
Length:857
Mass (Da):95,972
Last modified:December 1, 2001 - v1
Checksum:i2D34E3A0BCE47C8A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006914 Genomic DNA Translation: AAL02597.1
PIRiC97707
RefSeqiWP_010976745.1, NC_003103.1

Genome annotation databases

EnsemblBacteriaiAAL02597; AAL02597; RC0059
GeneIDi928600
KEGGirco:RC0059
PATRICifig|272944.4.peg.70

Entry informationi

Entry nameiCLPB_RICCN
AccessioniPrimary (citable) accession number: Q92JK8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: December 1, 2001
Last modified: March 28, 2018
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Rickettsia conorii
    (strain Malish 7): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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