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Q92JK6

- TSAD_RICCN

UniProt

Q92JK6 - TSAD_RICCN

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Protein

tRNA N6-adenosine threonylcarbamoyltransferase

Gene

tsaD

Organism
Rickettsia conorii (strain ATCC VR-613 / Malish 7)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.UniRule annotation

Catalytic activityi

L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Binds 1 Fe2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi112 – 1121IronUniRule annotation
Metal bindingi116 – 1161IronUniRule annotation
Binding sitei167 – 1671SubstrateUniRule annotation
Binding sitei180 – 1801Substrate; via amide nitrogenUniRule annotation
Binding sitei280 – 2801SubstrateUniRule annotation
Metal bindingi308 – 3081IronUniRule annotation

GO - Molecular functioni

  1. iron ion binding Source: UniProtKB-HAMAP
  2. metalloendopeptidase activity Source: InterPro
  3. transferase activity, transferring acyl groups other than amino-acyl groups Source: UniProtKB-HAMAP

GO - Biological processi

  1. threonylcarbamoyladenosine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.6.99.4UniRule annotation)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthaseUniRule annotation
Short name:
t(6)A synthaseUniRule annotation
t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
tRNA threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
Gene namesi
Name:tsaDUniRule annotation
Synonyms:gcp
Ordered Locus Names:RC0061
OrganismiRickettsia conorii (strain ATCC VR-613 / Malish 7)
Taxonomic identifieri272944 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000000816: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 344344tRNA N6-adenosine threonylcarbamoyltransferasePRO_0000303523Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272944.RC0061.

Structurei

3D structure databases

ProteinModelPortaliQ92JK6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni134 – 1385Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the KAE1 / TsaD family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0533.
HOGENOMiHOG000109568.
KOiK01409.
OMAiRDHVKRM.
OrthoDBiEOG6K402S.

Family and domain databases

HAMAPiMF_01445. TsaD.
InterProiIPR000905. Gcp-like_dom.
IPR017861. KAE1/YgjD.
IPR017860. Peptidase_M22_CS.
IPR022450. TsaD.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PRINTSiPR00789. OSIALOPTASE.
TIGRFAMsiTIGR00329. gcp_kae1. 1 hit.
TIGR03723. T6A_YgjD. 1 hit.
PROSITEiPS01016. GLYCOPROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q92JK6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIKILGIESS CDDTAVSIIT ENREILSNII ISQNTEHAVF GGVVPEIAAR
60 70 80 90 100
SHLSHLDKAL KNVLKESNTK LTDISTIAAT SGPGLIGGVI VGSMFARSLS
110 120 130 140 150
SALKKPFIAI NHLEGHALTA RLTDNIPYPY LLLLASGGHC QFVAVLGLGK
160 170 180 190 200
YKILGSTIDD AVGEAFDKVA KMLNLAFPGG PEIEKRAKLG DPHKYKFPKP
210 220 230 240 250
IINSGNCNMS FSGLKTAVRT LIMTLKEIND TVINDIAASF QFTIGEILSS
260 270 280 290 300
KVQDAIRAYE QITNNFDKKN IVIAGGVAAN KYLQKILSSC AKTYGYRLIY
310 320 330 340
PPIHLCTDNA AMIAYAGLER YNNKLFTPLN FCPKARWSLE DISN
Length:344
Mass (Da):37,302
Last modified:December 1, 2001 - v1
Checksum:i72B082999B0B7408
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE006914 Genomic DNA. Translation: AAL02599.1.
PIRiE97707.
RefSeqiNP_359698.1. NC_003103.1.

Genome annotation databases

EnsemblBacteriaiAAL02599; AAL02599; RC0061.
GeneIDi928604.
KEGGirco:RC0061.
PATRICi17887216. VBIRicCon45613_0072.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE006914 Genomic DNA. Translation: AAL02599.1 .
PIRi E97707.
RefSeqi NP_359698.1. NC_003103.1.

3D structure databases

ProteinModelPortali Q92JK6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272944.RC0061.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL02599 ; AAL02599 ; RC0061 .
GeneIDi 928604.
KEGGi rco:RC0061.
PATRICi 17887216. VBIRicCon45613_0072.

Phylogenomic databases

eggNOGi COG0533.
HOGENOMi HOG000109568.
KOi K01409.
OMAi RDHVKRM.
OrthoDBi EOG6K402S.

Family and domain databases

HAMAPi MF_01445. TsaD.
InterProi IPR000905. Gcp-like_dom.
IPR017861. KAE1/YgjD.
IPR017860. Peptidase_M22_CS.
IPR022450. TsaD.
[Graphical view ]
Pfami PF00814. Peptidase_M22. 1 hit.
[Graphical view ]
PRINTSi PR00789. OSIALOPTASE.
TIGRFAMsi TIGR00329. gcp_kae1. 1 hit.
TIGR03723. T6A_YgjD. 1 hit.
PROSITEi PS01016. GLYCOPROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC VR-613 / Malish 7.

Entry informationi

Entry nameiTSAD_RICCN
AccessioniPrimary (citable) accession number: Q92JK6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: December 1, 2001
Last modified: October 29, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Rickettsia conorii
    (strain Malish 7): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3