Q92JK0 (TILS_RICCN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA(Ile)-lysidine synthase
EC=6.3.4.19

Alternative name(s):
tRNA(Ile)-2-lysyl-cytidine synthase
tRNA(Ile)-lysidine synthetase

Gene names

Name:	tilS
Ordered Locus Names:	RC0067

Organism

Rickettsia conorii (strain ATCC VR-613 / Malish 7) [Complete proteome] [HAMAP]

Taxonomic identifier

272944 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	478 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine By similarity. HAMAP MF_01161
Catalytic activity	(tRNA(Ile2))-cytidine(34) + L-lysine + ATP = (tRNA(Ile2))-lysidine(34) + AMP + diphosphate + H2O. HAMAP MF_01161
Subcellular location	Cytoplasm Probable HAMAP MF_01161.
Domain	The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding. HAMAP MF_01161
Sequence similarities	Belongs to the tRNA(Ile)-lysidine synthase family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ligase activity, forming carbon-nitrogen bonds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 478	478	tRNA(Ile)-lysidine synthase HAMAP MF_01161		PRO_0000181756
Regions
	Nucleotide binding	27 – 32	6	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q92JK0 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: A169B1D28E9EE7CE	FASTA	478	55,546
	10 20 30 40 50 60 MLYEKFEYNI NNLIGNFGLS KISIAVSGGS DSVALLYLAN IWAEKNNIEL FVISVDHNLR 70 80 90 100 110 120 EQSKQETHYI QNISNSLNRK HYSLSFDHQN NFSNLQERAR EGRYDLMTNL CLELDILVLL 130 140 150 160 170 180 TAHHEDDYVE NFCLRLERNS GIFGLSSSNI NWYNNIQIIR PLYNIPKSEL VEYLVRHNIK 190 200 210 220 230 240 WFEDESNSSD KYRRNVIRQK LAKGADYIRH FSKPVYREEF KGDTERSTAA YTLVREDAST 250 260 270 280 290 300 GTASKLSLEA KCGKMSKAAI ISQQLKTNKL IENEFKPELI SAIAEAVKIF EYGFAFLDLV 310 320 330 340 350 360 KFDKFSNEVK VQIINFLLII ISGQSRAARF YSVEPILKLI TQDVNFKNTL HGCIIKRIQN 370 380 390 400 410 420 ELLIYREFGK KLPESKILLD KSVIWDNRFC ITKNQETPNC FVTHLSLKDY KIIKKQLDLE 430 440 450 460 470 PLKNLSCKNH NAVLLTLPII KILEKVIAIP HISYYDNDMW NFEVSFSPNF VSRFTHFC

References

[1]

"Mechanisms of evolution in Rickettsia conorii and R. prowazekii." Ogata H., Audic S., Renesto-Audiffren P., Fournier P.-E., Barbe V., Samson D., Roux V., Cossart P., Weissenbach J., Claverie J.-M., Raoult D. Science 293:2093-2098(2001) [PubMed: 11557893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-613 / Malish 7.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006914 Genomic DNA. Translation: AAL02605.1.
PIR	C97708.
RefSeq	NP_359704.1. NC_003103.1.
3D structure databases
ProteinModelPortal	Q92JK0.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	928597.
GenomeReviews	Gene locus RC0067 in contig AE006914_GR.
KEGG	rco:RC0067.
NMPDR	fig|272944.1.peg.67.
PATRIC	17887228. VBIRicCon45613_0078.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	NIQIIRP.
ProtClustDB	CLSK870716.
Enzyme and pathway databases
BioCyc	RCON272944:RC0067-MONOMER.
Family and domain databases
HAMAP	MF_01161. tRNA(Ile)-lys_synt. [Tree]
InterPro	IPR012094. Lysidine-tRNA-synth. IPR012795. Lysidine-tRNA-synth_N. IPR005728. Rickett_RPE. IPR014729. Rossmann-like_a/b/a_fold. IPR011063. tRNA-lysidine/thiocyt_synth. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K04075.
PANTHER	PTHR11807:SF2. ATPase_MesJ_YaeO. 1 hit.
Pfam	PF01171. ATP_bind_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR02432. Lysidine_TilS_N. 1 hit. TIGR01045. RPE1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TILS_RICCN

Accession

Primary (citable) accession number: Q92JK0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	December 1, 2001
Last modified:	January 25, 2012
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program