Reviewed, UniProtKB/Swiss-Prot Q92JI7 (DEF2_RICCN)

Names and origin

Protein names

Recommended name:
    Peptide deformylase 2
      Short name=PDF 2
    EC=3.5.1.88
Alternative name(s):
    Polypeptide deformylase 2

Gene names

Name:	def2
Ordered Locus Names:	RC0080

Organism

Rickettsia conorii [Complete proteome] [HAMAP]

Taxonomic identifier

781 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	202 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions By similarity.
Catalytic activity	Formyl-L-methionyl peptide + H2O = formate + methionyl peptide. HAMAP MF_00163
Cofactor	Binds 1 Fe2+ ion By similarity.
Sequence similarities	Belongs to the polypeptide deformylase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	Iron Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW peptide deformylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 202	202	Peptide deformylase 2 HAMAP MF_00163		PRO_0000082830
Sites
	Active site	163	1	By similarity
	Metal binding	120	1	Iron By similarity
	Metal binding	162	1	Iron By similarity
	Metal binding	166	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q92JI7-1 [UniParc]. Last modified September 19, 2002. Version 2. Checksum: 9A108195359D1B82	FASTA	202	23,191
	10 20 30 40 50 60 MKNENNISLP QYVTLNIQTL NSSEDKTIRI KAKTLNFPLS SEDLRDISIL EKKYDQEENC 70 80 90 100 110 120 AGLAAPQIGI SKCIIIFAVH EDAELKKWHP DLKDTMPKTI WINPSYKPIG IDKHEDYEGC 130 140 150 160 170 180 FSVENATGPV ARFKKIHYHA YDINGNQIQG IAEGFLARVI QHEIDHLNGK VFLDYVAPKK 190 200 IMTKEEYLEM RKKAMEQENI KS

References

[1]

"Mechanisms of evolution in Rickettsia conorii and R. prowazekii." Ogata H., Audic S., Renesto-Audiffren P., Fournier P.-E., Barbe V., Samson D., Roux V., Cossart P., Weissenbach J., Claverie J.-M., Raoult D. Science 293:2093-2098(2001) [PubMed: 11557893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-613 / Malish 7.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE006914 Genomic DNA. Translation: AAL02618.1. Different initiation.
PIR	H97709.
RefSeq	NP_359717.1.
3D structure databases
HSSP	HSSP built from PDB template 1LMH based on UniProtKB Q9F4L4.
ModBase	Search...
Genome annotation databases
GeneID	928534.
GenomeReviews	Gene locus RC0080 in contig AE006914_GR.
KEGG	rco:RC0080.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q92JI7.
Enzyme and pathway databases
BioCyc	RCON272944:RC0080-MON.
BRENDA	3.5.1.88. 267518.
Family and domain databases
HAMAP	MF_00163. [Tree]
InterPro	IPR000181. Fmet_deformylase. [Graphical view]
Gene3D	G3DSA:3.90.45.10. Fmet_deformylase. 1 hit.
PANTHER	PTHR10458. Fmet_deformylase. 1 hit.
Pfam	PF01327. Pep_deformylase. 1 hit. [Graphical view]
PIRSF	PIRSF004749. Pep_def. 1 hit.
PRINTS	PR01576. PDEFORMYLASE.
ProDom	PD003844. Fmet_deformylase. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

DEF2_RICCN

Accession

Primary (citable) accession number: Q92JI7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2002
Last sequence update:	September 19, 2002
Last modified:	June 16, 2009
This is version 41 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)