Q92JI3 (MNMG_RICCN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG

Alternative name(s):
Glucose-inhibited division protein A

Gene names

Name:	mnmG
Synonyms:	gidA
Ordered Locus Names:	RC0084

Organism

Rickettsia conorii (strain ATCC VR-613 / Malish 7) [Complete proteome] [HAMAP]

Taxonomic identifier

272944 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group › Rickettsia conorii

Protein attributes

Sequence length	622 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34 By similarity. HAMAP-Rule MF_00129
Cofactor	FAD By similarity.
Subunit structure	Homodimer By similarity. Heterotetramer of two MnmE and two MnmG subunits By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the MnmG family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NAD
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tRNA wobble uridine modification Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	flavin adenine dinucleotide binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 622	622	tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG HAMAP-Rule MF_00129		PRO_0000117164
Regions
	Nucleotide binding	10 – 15	6	FAD By similarity
	Nucleotide binding	269 – 283	15	NAD Potential
Sites
	Binding site	122	1	FAD; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	177	1	FAD By similarity
	Binding site	366	1	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q92JI3 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: D5660A0164A2A03A	FASTA	622	68,801
	10 20 30 40 50 60 MLKYDVIVIG GGHAGVEAAA ASARLGVPTL LITLKPENLG EMSCNPAIGG IAKGTLVKEI 70 80 90 100 110 120 DALDGLMGYV IDQAGIHYKM LNETRGPAVW GPRAQADRKL YKKAMYQILT NYPNLDILYG 130 140 150 160 170 180 KVEDIEIKSS KIEAVILNNG SKILCQKIIL TTGTFLSGLI HIGQKKIPAG RVDEEPSYGL 190 200 210 220 230 240 SNTLKQIGFK LARLKTGTPP RIDGRTIDYS KTILQPGDKI PRPFSELTNI VNVSQINCFI 250 260 270 280 290 300 TKTTSETHDI IRENLDKSAM YSGQIEGIGP RYCPSIEDKI VRFSTKSEHR IFLEPEGLDD 310 320 330 340 350 360 YTIYPNGIST SLPEDVQHKL IKTIPGLENV KVLRPGYAIE YDYVDPREIS VTLETKKIAG 370 380 390 400 410 420 LYLAGQINGT TGYEEAAGQG IIAGINAALA VKDQAPFMLT RANSYIGVMI DDLTTFGTIE 430 440 450 460 470 480 PYRMFTSRSE YRLSLRADNS DLRLTELGMN IGVVSEKRKK IFTKKCEDIE KIKSLLNTLS 490 500 510 520 530 540 LSTSKLAKMG IQVAQDGTYK TVLDLFKIPN FNVEQAIKIF PMLKETQNNN ILQLLYIEAK 550 560 570 580 590 600 YASYLTRQHA DINLFQSEEA QFIPKNIDYF KIPSISLEIQ EKLSSHKPTT IGVARRIPGI 610 620 TPAAITAIII YLKTKYSDGS ST

References

[1]

"Mechanisms of evolution in Rickettsia conorii and R. prowazekii." Ogata H., Audic S., Renesto-Audiffren P., Fournier P.-E., Barbe V., Samson D., Roux V., Cossart P., Weissenbach J., Claverie J.-M., Raoult D. Science 293:2093-2098(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-613 / Malish 7.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006914 Genomic DNA. Translation: AAL02622.1.
PIR	D97710.
RefSeq	NP_359721.1. NC_003103.1.
3D structure databases
ProteinModelPortal	Q92JI3.
ModBase	Search...
Protein-protein interaction databases
STRING	272944.RC0084.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL02622; AAL02622; RC0084.
GeneID	928530.
KEGG	rco:RC0084.
PATRIC	17887274. VBIRicCon45613_0101.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0445.
HOGENOM	HOG000201059.
KO	K03495.
OMA	AQMSCNP.
ProtClustDB	PRK05192.
Family and domain databases
HAMAP	MF_00129. MnmG_GidA.
InterPro	IPR004416. GidA. IPR026904. GidA-assoc_3. IPR002218. GIDA-rel. IPR020595. GIDA-rel_CS. [Graphical view]
Pfam	PF01134. GIDA. 1 hit. PF13932. GIDA_assoc_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00136. gidA. 1 hit.
PROSITE	PS01280. GIDA_1. 1 hit. PS01281. GIDA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MNMG_RICCN

Accession

Primary (citable) accession number: Q92JI3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program