Reviewed, UniProtKB/Swiss-Prot Q92JI3 (MNMG_RICCN)

Names and origin

Protein names

Recommended name:
    tRNA uridine 5-carboxymethylaminomethyl modification enzyme mnmG
Alternative name(s):
    Glucose-inhibited division protein A

Gene names

Name:	mnmG
Synonyms:	gidA
Ordered Locus Names:	RC0084

Organism

Rickettsia conorii [Complete proteome] [HAMAP]

Taxonomic identifier

781 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	622 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34 By similarity.
Cofactor	FAD By similarity.
Subunit structure	Homodimer By similarity. Heterotetramer of two mnmE and two mnmG subunits By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the mnmG family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NAD
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA wobble uridine modification Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: HAMAP electron carrier activity Inferred from electronic annotation. Source: InterPro oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 622	622	tRNA uridine 5-carboxymethylaminomethyl modification enzyme mnmG HAMAP MF_00129		PRO_0000117164
Regions
	Nucleotide binding	10 – 15	6	FAD By similarity
	Nucleotide binding	269 – 283	15	NAD Potential
Sites
	Binding site	122	1	FAD; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	177	1	FAD By similarity
	Binding site	366	1	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q92JI3-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: D5660A0164A2A03A	FASTA	622	68,801
	10 20 30 40 50 60 MLKYDVIVIG GGHAGVEAAA ASARLGVPTL LITLKPENLG EMSCNPAIGG IAKGTLVKEI 70 80 90 100 110 120 DALDGLMGYV IDQAGIHYKM LNETRGPAVW GPRAQADRKL YKKAMYQILT NYPNLDILYG 130 140 150 160 170 180 KVEDIEIKSS KIEAVILNNG SKILCQKIIL TTGTFLSGLI HIGQKKIPAG RVDEEPSYGL 190 200 210 220 230 240 SNTLKQIGFK LARLKTGTPP RIDGRTIDYS KTILQPGDKI PRPFSELTNI VNVSQINCFI 250 260 270 280 290 300 TKTTSETHDI IRENLDKSAM YSGQIEGIGP RYCPSIEDKI VRFSTKSEHR IFLEPEGLDD 310 320 330 340 350 360 YTIYPNGIST SLPEDVQHKL IKTIPGLENV KVLRPGYAIE YDYVDPREIS VTLETKKIAG 370 380 390 400 410 420 LYLAGQINGT TGYEEAAGQG IIAGINAALA VKDQAPFMLT RANSYIGVMI DDLTTFGTIE 430 440 450 460 470 480 PYRMFTSRSE YRLSLRADNS DLRLTELGMN IGVVSEKRKK IFTKKCEDIE KIKSLLNTLS 490 500 510 520 530 540 LSTSKLAKMG IQVAQDGTYK TVLDLFKIPN FNVEQAIKIF PMLKETQNNN ILQLLYIEAK 550 560 570 580 590 600 YASYLTRQHA DINLFQSEEA QFIPKNIDYF KIPSISLEIQ EKLSSHKPTT IGVARRIPGI 610 620 TPAAITAIII YLKTKYSDGS ST

References

[1]

"Mechanisms of evolution in Rickettsia conorii and R. prowazekii." Ogata H., Audic S., Renesto-Audiffren P., Fournier P.-E., Barbe V., Samson D., Roux V., Cossart P., Weissenbach J., Claverie J.-M., Raoult D. Science 293:2093-2098(2001) [PubMed: 11557893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-613 / Malish 7.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE006914 Genomic DNA. Translation: AAL02622.1.
PIR	D97710.
RefSeq	NP_359721.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	928530.
GenomeReviews	Gene locus RC0084 in contig AE006914_GR.
KEGG	rco:RC0084.
NMPDR	fig|272944.1.peg.84.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	IQFRVLN.
Enzyme and pathway databases
BioCyc	RCON272944:RC0084-MON.
Family and domain databases
HAMAP	MF_00129. [Tree]
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR004416. GidA. IPR002218. GIDA-rel. IPR020595. GIDA-rel_CS. [Graphical view]
Pfam	PF01134. GIDA. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR.
PROSITE	PS01280. GIDA_1. 1 hit. PS01281. GIDA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MNMG_RICCN

Accession

Primary (citable) accession number: Q92JI3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	December 1, 2001
Last modified:	December 15, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)