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Q92JG5 (QUEF_RICCN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductase

EC=1.7.1.13
Alternative name(s):
7-cyano-7-carbaguanine reductase
NADPH-dependent nitrile oxidoreductase
PreQ(0) reductase
Gene names
Name:queF
Ordered Locus Names:RC0102
OrganismRickettsia conorii (strain ATCC VR-613 / Malish 7) [Complete proteome] [HAMAP]
Taxonomic identifier272944 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group

Protein attributes

Sequence length273 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity. HAMAP-Rule MF_00817

Catalytic activity

7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP-Rule MF_00817

Pathway

tRNA modification; tRNA-queuosine biosynthesis. HAMAP-Rule MF_00817

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00817

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00817.

Sequence similarities

Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Ontologies

Keywords
   Biological processQueuosine biosynthesis
   Cellular componentCytoplasm
   LigandNADP
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processqueuosine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionoxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor

Inferred from electronic annotation. Source: InterPro

preQ1 synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 273273NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP-Rule MF_00817
PRO_0000163052

Regions

Nucleotide binding83 – 842NADPH By similarity
Nucleotide binding247 – 2482NADPH By similarity
Region81 – 833Substrate binding By similarity
Region218 – 2192Substrate binding By similarity

Sites

Active site1791Thioimide intermediate By similarity
Active site1861Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q92JG5 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: B31146994074D23B

FASTA27331,215
        10         20         30         40         50         60 
MPLSTSLLGK KSTYKDSYDV TLLFKIPRIN NRNELGINSN NLPFYGVDVW NTYELSCLNK 

        70         80         90        100        110        120 
NGKPWVGVGT FYIPTDSENI VESKSFKLYL NSFNNFVVES VKELERIILQ DLSNVTHAKV 

       130        140        150        160        170        180 
TGRIFPINTK VEFGVPSGKN IDDLDIVCNN YGAPDNSLIE YEDVLVEEEI NSHLLKSNCL 

       190        200        210        220        230        240 
VTGQPDWGTI VIKYKGKKLK YDSFLKYLIS FRNCNEFAEQ CAERIFTDIK NAISPDFLSI 

       250        260        270 
YIVYARRGGI DICPYRSTDK SYTLPSDKRF IRQ 

« Hide

References

[1]"Mechanisms of evolution in Rickettsia conorii and R. prowazekii."
Ogata H., Audic S., Renesto-Audiffren P., Fournier P.-E., Barbe V., Samson D., Roux V., Cossart P., Weissenbach J., Claverie J.-M., Raoult D.
Science 293:2093-2098(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC VR-613 / Malish 7.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE006914 Genomic DNA. Translation: AAL02640.1.
PIRF97712.
RefSeqNP_359739.1. NC_003103.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272944.RC0102.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL02640; AAL02640; RC0102.
GeneID928092.
KEGGrco:RC0102.
PATRIC17887314. VBIRicCon45613_0121.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0780.
HOGENOMHOG000273755.
KOK06879.
OMAQCVERIY.
OrthoDBEOG6D5G0M.
ProtClustDBPRK11792.

Enzyme and pathway databases

UniPathwayUPA00392.

Family and domain databases

HAMAPMF_00817. QueF_type2.
InterProIPR016428. QueF_type2.
[Graphical view]
PIRSFPIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMsTIGR03138. QueF. 1 hit.
ProtoNetSearch...

Entry information

Entry nameQUEF_RICCN
AccessionPrimary (citable) accession number: Q92JG5
Entry history
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: December 1, 2001
Last modified: April 16, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rickettsia conorii

(strain Malish 7): entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways