Reviewed, UniProtKB/Swiss-Prot Q92JG5 (QUEF_RICCN)

Names and origin

Protein names

Recommended name:
    NADPH-dependent 7-cyano-7-deazaguanine reductase
    EC=1.7.1.13
Alternative name(s):
    7-cyano-7-carbaguanine reductase
    PreQ(0) reductase
    NADPH-dependent nitrile oxidoreductase

Gene names

Name:	queF
Ordered Locus Names:	RC0102

Organism

Rickettsia conorii [Complete proteome] [HAMAP]

Taxonomic identifier

781 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity.
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00817
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00817
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP queuine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 273	273	NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00817		PRO_0000163052

Sequences

	Sequence		Length	Mass (Da)
	Q92JG5-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: B31146994074D23B	FASTA	273	31,215
	10 20 30 40 50 60 MPLSTSLLGK KSTYKDSYDV TLLFKIPRIN NRNELGINSN NLPFYGVDVW NTYELSCLNK 70 80 90 100 110 120 NGKPWVGVGT FYIPTDSENI VESKSFKLYL NSFNNFVVES VKELERIILQ DLSNVTHAKV 130 140 150 160 170 180 TGRIFPINTK VEFGVPSGKN IDDLDIVCNN YGAPDNSLIE YEDVLVEEEI NSHLLKSNCL 190 200 210 220 230 240 VTGQPDWGTI VIKYKGKKLK YDSFLKYLIS FRNCNEFAEQ CAERIFTDIK NAISPDFLSI 250 260 270 YIVYARRGGI DICPYRSTDK SYTLPSDKRF IRQ

References

[1]

"Mechanisms of evolution in Rickettsia conorii and R. prowazekii." Ogata H., Audic S., Renesto-Audiffren P., Fournier P.-E., Barbe V., Samson D., Roux V., Cossart P., Weissenbach J., Claverie J.-M., Raoult D. Science 293:2093-2098(2001) [PubMed: 11557893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-613 / Malish 7.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE006914 Genomic DNA. Translation: AAL02640.1.
PIR	F97712.
RefSeq	NP_359739.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	928092.
GenomeReviews	Gene locus RC0102 in contig AE006914_GR.
KEGG	rco:RC0102.
NMPDR	fig|272944.1.peg.102.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	INPFRSN.
Enzyme and pathway databases
BioCyc	RCON272944:RC0102-MON.
BRENDA	1.7.1.13. 267518.
Family and domain databases
HAMAP	MF_00817. [Tree]
InterPro	IPR016428. CN_OxRdtase_NADPH-dep_YqcD. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. [Graphical view]
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_RICCN

Accession

Primary (citable) accession number: Q92JG5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	December 1, 2001
Last modified:	December 15, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)