Reviewed, UniProtKB/Swiss-Prot Q92JF6 (SYC_RICCN)

Names and origin

Protein names

Recommended name:
    Cysteinyl-tRNA synthetase
    EC=6.1.1.16
Alternative name(s):
    Cysteine--tRNA ligase
      Short name=CysRS

Gene names

Name:	cysS
Ordered Locus Names:	RC0111

Organism

Rickettsia conorii [Complete proteome] [HAMAP]

Taxonomic identifier

781 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	459 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm HAMAP MF_00041.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP cysteine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 459	459	Cysteinyl-tRNA synthetase HAMAP MF_00041		PRO_0000159468
Regions
	Motif	33 – 43	11	"HIGH" region HAMAP MF_00041
	Motif	274 – 278	5	"KMSKS" region HAMAP MF_00041
Sites
	Metal binding	31	1	Zinc By similarity
	Metal binding	216	1	Zinc By similarity
	Metal binding	241	1	Zinc By similarity
	Metal binding	245	1	Zinc By similarity
	Binding site	277	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q92JF6-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: AB3CD491F7830228	FASTA	459	52,998
	10 20 30 40 50 60 MQIQFHLYNT LSRTKEVFNP QDQANVKMYV CGPTVYYNPH IGNSRSGVVY DLLYRIVIKI 70 80 90 100 110 120 FGEKAVKYVR NITDVDDKII DRAALLGVTI DELTDKVTKE FHKNMAYLGC MLPSIEPKAT 130 140 150 160 170 180 KHIDVMIAII ERLIAKDHAY IADNHVYFDV LSAPNYTELS NRNLEEMFEG VHVENSKTKK 190 200 210 220 230 240 NPQDFVLWKP AKQNESANMN FESPWGLGRP GWHIECSAMS YKYLGENFDI HGGGADLIFP 250 260 270 280 290 300 HHTNEIAQSR CAFPSSTYAK YWVHNGFLTV NGEKMSKSLG NFITVRDLMD KQIQGEVVRL 310 320 330 340 350 360 FLLSSHYRRP LDYNDKAIED AKKTLDYWYR AIENINVQKI DLPHDFMQSL LDDMNTPLAV 370 380 390 400 410 420 KIINDYAKGV FISKTEEERQ LNASAIITCA NFIGLMNKTP HEWFNSGVDE LYVNELVNKR 430 440 450 LEAKKQKNWL LADQIRNQLL EEKIILEDRP DGTTIWRKE

References

[1]

"Mechanisms of evolution in Rickettsia conorii and R. prowazekii." Ogata H., Audic S., Renesto-Audiffren P., Fournier P.-E., Barbe V., Samson D., Roux V., Cossart P., Weissenbach J., Claverie J.-M., Raoult D. Science 293:2093-2098(2001) [PubMed: 11557893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-613 / Malish 7.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE006914 Genomic DNA. Translation: AAL02649.1.
PIR	G97713.
RefSeq	NP_359748.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	928083.
GenomeReviews	Gene locus RC0111 in contig AE006914_GR.
KEGG	rco:RC0111.
NMPDR	fig|272944.1.peg.111.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	VLWKAAK.
Enzyme and pathway databases
BioCyc	RCON272944:RC0111-MON.
BRENDA	6.1.1.16. 267518.
Family and domain databases
HAMAP	MF_00041. [Tree]
InterPro	IPR015804. Cys-tRNA-synt_Ia_C. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR015803. Cys-tRNA-synt_Ia_N. IPR002308. Cys-tRNA-synth_1a. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
SMART	SM00840. DALR_2. 1 hit. [Graphical view]
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_RICCN

Accession

Primary (citable) accession number: Q92JF6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	December 1, 2001
Last modified:	December 15, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)