Q92JE9 (KDTA_RICCN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-deoxy-D-manno-octulosonic acid transferase
Short name=Kdo transferase
EC=2.4.99.12

Alternative name(s):
Lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase

Gene names

Name:	waaA
Synonyms:	kdtA
Ordered Locus Names:	RC0118

Organism

Rickettsia conorii (strain ATCC VR-613 / Malish 7) [Complete proteome] [HAMAP]

Taxonomic identifier

272944 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group › Rickettsia conorii

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in lipopolysaccharide (LPS) biosynthesis. Catalyzes the transfer of 3-deoxy-D-manno-octulosonate (Kdo) residue(s) from CMP-Kdo to lipid IV(A), the tetraacyldisaccharide-1,4'-bisphosphate precursor of lipid A By similarity.
Catalytic activity	Lipid IV(A) + CMP-alpha-Kdo = alpha-Kdo-(2->6)-lipid IV(A) + CMP.
Pathway	Bacterial outer membrane biogenesis; LPS core biosynthesis.
Subcellular location	Cell inner membrane; Single-pass membrane protein; Cytoplasmic side By similarity.
Sequence similarities	Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 30 subfamily. Contains 1 RPE1 insert domain.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	lipopolysaccharide core region biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 464	464	3-deoxy-D-manno-octulosonic acid transferase		PRO_0000286455
Regions
	Transmembrane	2 – 22	21	Helical; Signal-anchor; Potential
	Domain	47 – 93	47	RPE1 insert
	Region	311 – 312	2	CMP-Kdo binding By similarity
	Region	352 – 354	3	CMP-Kdo binding By similarity
	Region	377 – 380	4	CMP-Kdo binding By similarity
Sites
	Active site	107	1	Proton acceptor By similarity
	Site	177	1	Transition state stabilizer By similarity
	Site	252	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q92JE9 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 476C023C396CFCFA	FASTA	464	53,047
	10 20 30 40 50 60 MMLLYYALSF ILLPVYFIII LIRLLIGKED IRRIQERFAI GKHRQDDSLD FMQTSANKEE 70 80 90 100 110 120 FKGDTSLRTT TYTLIREDEG LGSTYKLPLE ASDARRLIWI NAASIGESMV ALTLIHNISK 130 140 150 160 170 180 RYPDVRFLVT SWTNSSAKIL TAKLPKIAVH QFLPIDNIIF TRKFLRNWQP DLGIFIESEL 190 200 210 220 230 240 WPCTINEGAK QCKLLLVNAR ISDKSFKAWL QRKSFFQLIL KNCSKIIVQS ERDLQKFNEL 250 260 270 280 290 300 GVSDAVNLGN IKFANEKLPV NQEELSKLSL HLDNKRVVLF ASTHPEDEEV ILPIIKNLKE 310 320 330 340 350 360 QFLDCYIILI PRHPERVKSI IDNCKSHNLS ATAKSQNDLP VLSDDLYIVD RFGEMGLFFS 370 380 390 400 410 420 VATISFIGGS FKQGGHNILE AAYFSNCIIF GPDMSKNTDI AKGVLQNEAA IQIKNGEDLL 430 440 450 460 TKLTYLLRSN NALELTTYRE NALKFIKDNQ KVLDEYLNVI TKFL

References

[1]

"Mechanisms of evolution in Rickettsia conorii and R. prowazekii." Ogata H., Audic S., Renesto-Audiffren P., Fournier P.-E., Barbe V., Samson D., Roux V., Cossart P., Weissenbach J., Claverie J.-M., Raoult D. Science 293:2093-2098(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-613 / Malish 7.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006914 Genomic DNA. Translation: AAL02656.1.
PIR	F97714.
RefSeq	NP_359755.1. NC_003103.1.
3D structure databases
ProteinModelPortal	Q92JE9.
ModBase	Search...
Protein-protein interaction databases
STRING	272944.RC0118.
Protein family/group databases
CAZy	GT30. Glycosyltransferase Family 30.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL02656; AAL02656; RC0118.
GeneID	928074.
KEGG	rco:RC0118.
PATRIC	17887355. VBIRicCon45613_0140.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1519.
HOGENOM	HOG000010628.
KO	K02527.
OMA	KHGGHNP.
ProtClustDB	PRK05749.
Enzyme and pathway databases
UniPathway	UPA00958.
Family and domain databases
InterPro	IPR007507. Glycos_transf_N. IPR005728. Rickett_RPE. [Graphical view]
Pfam	PF04413. Glycos_transf_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01045. RPE1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KDTA_RICCN

Accession

Primary (citable) accession number: Q92JE9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program