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Protein

3-deoxy-D-manno-octulosonic acid transferase

Gene

waaA

Organism
Rickettsia conorii (strain ATCC VR-613 / Malish 7)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in lipopolysaccharide (LPS) biosynthesis. Catalyzes the transfer of 3-deoxy-D-manno-octulosonate (Kdo) residue(s) from CMP-Kdo to lipid IV(A), the tetraacyldisaccharide-1,4'-bisphosphate precursor of lipid A (By similarity).By similarity

Catalytic activityi

Lipid IV(A) + CMP-alpha-Kdo = alpha-Kdo-(2->6)-lipid IV(A) + CMP.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei107 – 1071Proton acceptorBy similarity
Sitei177 – 1771Transition state stabilizerBy similarity
Sitei252 – 2521Transition state stabilizerBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Lipopolysaccharide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00958.

Protein family/group databases

CAZyiGT30. Glycosyltransferase Family 30.

Names & Taxonomyi

Protein namesi
Recommended name:
3-deoxy-D-manno-octulosonic acid transferase (EC:2.4.99.12)
Short name:
Kdo transferase
Alternative name(s):
Lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase
Gene namesi
Name:waaA
Synonyms:kdtA
Ordered Locus Names:RC0118
OrganismiRickettsia conorii (strain ATCC VR-613 / Malish 7)
Taxonomic identifieri272944 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000000816 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei2 – 2221Helical; Signal-anchorSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4644643-deoxy-D-manno-octulosonic acid transferasePRO_0000286455Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272944.RC0118.

Structurei

3D structure databases

ProteinModelPortaliQ92JE9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini47 – 9347RPE1 insertAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni311 – 3122CMP-Kdo bindingBy similarity
Regioni352 – 3543CMP-Kdo bindingBy similarity
Regioni377 – 3804CMP-Kdo bindingBy similarity

Sequence similaritiesi

Contains 1 RPE1 insert domain.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1519.
HOGENOMiHOG000010628.
KOiK02527.
OMAiKHGGHNP.
OrthoDBiEOG6H7FNN.

Family and domain databases

InterProiIPR007507. Glycos_transf_N.
IPR005728. Rickett_RPE.
[Graphical view]
PfamiPF04413. Glycos_transf_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01045. RPE1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q92JE9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMLLYYALSF ILLPVYFIII LIRLLIGKED IRRIQERFAI GKHRQDDSLD
60 70 80 90 100
FMQTSANKEE FKGDTSLRTT TYTLIREDEG LGSTYKLPLE ASDARRLIWI
110 120 130 140 150
NAASIGESMV ALTLIHNISK RYPDVRFLVT SWTNSSAKIL TAKLPKIAVH
160 170 180 190 200
QFLPIDNIIF TRKFLRNWQP DLGIFIESEL WPCTINEGAK QCKLLLVNAR
210 220 230 240 250
ISDKSFKAWL QRKSFFQLIL KNCSKIIVQS ERDLQKFNEL GVSDAVNLGN
260 270 280 290 300
IKFANEKLPV NQEELSKLSL HLDNKRVVLF ASTHPEDEEV ILPIIKNLKE
310 320 330 340 350
QFLDCYIILI PRHPERVKSI IDNCKSHNLS ATAKSQNDLP VLSDDLYIVD
360 370 380 390 400
RFGEMGLFFS VATISFIGGS FKQGGHNILE AAYFSNCIIF GPDMSKNTDI
410 420 430 440 450
AKGVLQNEAA IQIKNGEDLL TKLTYLLRSN NALELTTYRE NALKFIKDNQ
460
KVLDEYLNVI TKFL
Length:464
Mass (Da):53,047
Last modified:December 1, 2001 - v1
Checksum:i476C023C396CFCFA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006914 Genomic DNA. Translation: AAL02656.1.
PIRiF97714.
RefSeqiNP_359755.1. NC_003103.1.
WP_010976798.1. NC_003103.1.

Genome annotation databases

EnsemblBacteriaiAAL02656; AAL02656; RC0118.
GeneIDi928074.
KEGGirco:RC0118.
PATRICi17887355. VBIRicCon45613_0140.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006914 Genomic DNA. Translation: AAL02656.1.
PIRiF97714.
RefSeqiNP_359755.1. NC_003103.1.
WP_010976798.1. NC_003103.1.

3D structure databases

ProteinModelPortaliQ92JE9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272944.RC0118.

Protein family/group databases

CAZyiGT30. Glycosyltransferase Family 30.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL02656; AAL02656; RC0118.
GeneIDi928074.
KEGGirco:RC0118.
PATRICi17887355. VBIRicCon45613_0140.

Phylogenomic databases

eggNOGiCOG1519.
HOGENOMiHOG000010628.
KOiK02527.
OMAiKHGGHNP.
OrthoDBiEOG6H7FNN.

Enzyme and pathway databases

UniPathwayiUPA00958.

Family and domain databases

InterProiIPR007507. Glycos_transf_N.
IPR005728. Rickett_RPE.
[Graphical view]
PfamiPF04413. Glycos_transf_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01045. RPE1. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC VR-613 / Malish 7.

Entry informationi

Entry nameiKDTA_RICCN
AccessioniPrimary (citable) accession number: Q92JE9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: December 1, 2001
Last modified: May 27, 2015
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Rickettsia conorii
    (strain Malish 7): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.