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Q92JE9 (KDTA_RICCN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
3-deoxy-D-manno-octulosonic acid transferase

Short name=Kdo transferase
EC=2.4.99.12
Alternative name(s):
Lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase
Gene names
Name:waaA
Synonyms:kdtA
Ordered Locus Names:RC0118
OrganismRickettsia conorii (strain ATCC VR-613 / Malish 7) [Complete proteome] [HAMAP]
Taxonomic identifier272944 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group

Protein attributes

Sequence length464 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in lipopolysaccharide (LPS) biosynthesis. Catalyzes the transfer of 3-deoxy-D-manno-octulosonate (Kdo) residue(s) from CMP-Kdo to lipid IV(A), the tetraacyldisaccharide-1,4'-bisphosphate precursor of lipid A By similarity.

Catalytic activity

Lipid IV(A) + CMP-alpha-Kdo = alpha-Kdo-(2->6)-lipid IV(A) + CMP.

Pathway

Bacterial outer membrane biogenesis; LPS core biosynthesis.

Subcellular location

Cell inner membrane; Single-pass membrane protein; Cytoplasmic side By similarity.

Sequence similarities

Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 30 subfamily.

Contains 1 RPE1 insert domain.

Ontologies

Keywords
   Biological processLipopolysaccharide biosynthesis
   Cellular componentCell inner membrane
Cell membrane
Membrane
   DomainSignal-anchor
Transmembrane
Transmembrane helix
   Molecular functionTransferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processlipopolysaccharide core region biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontransferase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4644643-deoxy-D-manno-octulosonic acid transferase
PRO_0000286455

Regions

Transmembrane2 – 2221Helical; Signal-anchor; Potential
Domain47 – 9347RPE1 insert
Region311 – 3122CMP-Kdo binding By similarity
Region352 – 3543CMP-Kdo binding By similarity
Region377 – 3804CMP-Kdo binding By similarity

Sites

Active site1071Proton acceptor By similarity
Site1771Transition state stabilizer By similarity
Site2521Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
Q92JE9 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 476C023C396CFCFA

FASTA46453,047
        10         20         30         40         50         60 
MMLLYYALSF ILLPVYFIII LIRLLIGKED IRRIQERFAI GKHRQDDSLD FMQTSANKEE 

        70         80         90        100        110        120 
FKGDTSLRTT TYTLIREDEG LGSTYKLPLE ASDARRLIWI NAASIGESMV ALTLIHNISK 

       130        140        150        160        170        180 
RYPDVRFLVT SWTNSSAKIL TAKLPKIAVH QFLPIDNIIF TRKFLRNWQP DLGIFIESEL 

       190        200        210        220        230        240 
WPCTINEGAK QCKLLLVNAR ISDKSFKAWL QRKSFFQLIL KNCSKIIVQS ERDLQKFNEL 

       250        260        270        280        290        300 
GVSDAVNLGN IKFANEKLPV NQEELSKLSL HLDNKRVVLF ASTHPEDEEV ILPIIKNLKE 

       310        320        330        340        350        360 
QFLDCYIILI PRHPERVKSI IDNCKSHNLS ATAKSQNDLP VLSDDLYIVD RFGEMGLFFS 

       370        380        390        400        410        420 
VATISFIGGS FKQGGHNILE AAYFSNCIIF GPDMSKNTDI AKGVLQNEAA IQIKNGEDLL 

       430        440        450        460 
TKLTYLLRSN NALELTTYRE NALKFIKDNQ KVLDEYLNVI TKFL 

« Hide

References

[1]"Mechanisms of evolution in Rickettsia conorii and R. prowazekii."
Ogata H., Audic S., Renesto-Audiffren P., Fournier P.-E., Barbe V., Samson D., Roux V., Cossart P., Weissenbach J., Claverie J.-M., Raoult D.
Science 293:2093-2098(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC VR-613 / Malish 7.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE006914 Genomic DNA. Translation: AAL02656.1.
PIRF97714.
RefSeqNP_359755.1. NC_003103.1.

3D structure databases

ProteinModelPortalQ92JE9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272944.RC0118.

Protein family/group databases

CAZyGT30. Glycosyltransferase Family 30.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL02656; AAL02656; RC0118.
GeneID928074.
KEGGrco:RC0118.
PATRIC17887355. VBIRicCon45613_0140.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1519.
HOGENOMHOG000010628.
KOK02527.
OMAQHCYLPY.
OrthoDBEOG6H7FNN.
ProtClustDBPRK05749.

Enzyme and pathway databases

UniPathwayUPA00958.

Family and domain databases

InterProIPR007507. Glycos_transf_N.
IPR005728. Rickett_RPE.
[Graphical view]
PfamPF04413. Glycos_transf_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR01045. RPE1. 1 hit.
ProtoNetSearch...

Entry information

Entry nameKDTA_RICCN
AccessionPrimary (citable) accession number: Q92JE9
Entry history
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: December 1, 2001
Last modified: February 19, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rickettsia conorii

(strain Malish 7): entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways