Reviewed, UniProtKB/Swiss-Prot Q92JB1 (LEP_RICCN)

Names and origin

Protein names

Recommended name:
    Signal peptidase I
      Short name=SPase I
    EC=3.4.21.89
Alternative name(s):
    Leader peptidase I

Gene names

Name:	lepB
Ordered Locus Names:	RC0156

Organism

Rickettsia conorii [Complete proteome] [HAMAP]

Taxonomic identifier

781 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	266 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.
Subcellular location	Cell membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the peptidase S26 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	serine-type peptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 266	266	Signal peptidase I		PRO_0000109515
Regions
	Topological domain	1 – 20	20	Cytoplasmic Potential
	Transmembrane	21 – 41	21	Potential
	Topological domain	42 – 266	225	Extracellular Potential
Sites
	Active site	45	1	By similarity
	Active site	108	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q92JB1-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 7C3926FECAB482FA	FASTA	266	31,140
	10 20 30 40 50 60 MQTDNTKSNT NKTAKQEWGS FAFVICIALL IRILIMEPFN VPTGSMKATI LENDYIFSTK 70 80 90 100 110 120 YSYGYSNYSL SFFDFIPLFK GRIFAREPDR GDIVVFRPPN DMSVRYIKRL IGLPGDKIQL 130 140 150 160 170 180 IDDVIYINDK KIERTEVGTY ISEEGIKYLK FKETLPNGRT YFSYKLAPIY GVIYNDRYSN 190 200 210 220 230 240 TDVFYVPEGQ YFFLGDNRDQ SNDSRVNLGF VPFENFIAKA QFIWFSTKIT WWDNDIGVIN 250 260 LVLKLKPWIE SVRLNRIFRN LYNTDA

References

[1]

"Mechanisms of evolution in Rickettsia conorii and R. prowazekii." Ogata H., Audic S., Renesto-Audiffren P., Fournier P.-E., Barbe V., Samson D., Roux V., Cossart P., Weissenbach J., Claverie J.-M., Raoult D. Science 293:2093-2098(2001) [PubMed: 11557893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-613 / Malish 7.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE006914 Genomic DNA. Translation: AAL02694.1.
PIR	D97719.
RefSeq	NP_359793.1.
3D structure databases
HSSP	HSSP built from PDB template 1B12 based on UniProtKB P00803.
ModBase	Search...
Protein family/group databases
MEROPS	S26.001.
Genome annotation databases
GeneID	928036.
GenomeReviews	Gene locus RC0156 in contig AE006914_GR.
KEGG	rco:RC0156.
NMPDR	fig|272944.1.peg.156.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q92JB1.
OMA	KPWIESV.
Enzyme and pathway databases
BioCyc	RCON272944:RC0156-MON.
BRENDA	3.4.21.89. 267518.
Family and domain databases
InterPro	IPR000223. Pept_S26A_signal_pept_1. IPR019758. Pept_S26A_signal_pept_1_CS. IPR019757. Pept_S26A_signal_pept_1_Lys-AS. IPR019756. Pept_S26A_signal_pept_1_Ser-AS. IPR019759. Peptidase_S24_S26_cons-reg. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit.
Pfam	PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00727. LEADERPTASE.
TIGRFAMs	TIGR02227. sigpep_I_bact. 1 hit.
PROSITE	PS00501. SPASE_I_1. False negative. PS00760. SPASE_I_2. 1 hit. PS00761. SPASE_I_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEP_RICCN

Accession

Primary (citable) accession number: Q92JB1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	December 1, 2001
Last modified:	November 3, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)