Reviewed, UniProtKB/Swiss-Prot Q92J97 (DHSA_RICCN)

Names and origin

Protein names

Recommended name:
    Succinate dehydrogenase flavoprotein subunit
    EC=1.3.99.1

Gene names

Name:	sdhA
Ordered Locus Names:	RC0170

Organism

Rickettsia conorii [Complete proteome] [HAMAP]

Taxonomic identifier

781 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	596 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Succinate + acceptor = fumarate + reduced acceptor.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle.
Subunit structure	Part of an enzyme complex containing four subunits: a flavoprotein, an iron-sulfur, cytochrome b-556, and an hydrophobic anchor protein.
Subcellular location	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side.
Sequence similarities	Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.

Ontologies

Keywords
Biological process	Electron transport Transport Tricarboxylic acid cycle
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: InterPro
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro succinate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 596	596	Succinate dehydrogenase flavoprotein subunit		PRO_0000158656
Regions
	Nucleotide binding	41 – 56	16	FAD By similarity
Sites
	Active site	290	1	Proton acceptor By similarity
	Binding site	246	1	Substrate By similarity
	Binding site	258	1	Substrate By similarity
	Binding site	357	1	Substrate By similarity
	Binding site	402	1	Substrate By similarity
Amino acid modifications
	Modified residue	49	1	Tele-8alpha-FAD histidine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q92J97-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: F313CE9506A7DA10	FASTA	596	65,291
	10 20 30 40 50 60 MTKAYNIIHH KFDVVVVGAG GAGLRSAFGM AKEGLNTACI TKLFPTRSHT VAAQGGISAA 70 80 90 100 110 120 LGNMGEDDWR WHMYDTVKGS DWLGDQDAIE YMCKNAPDAI LELEHYGVPF SRTEEGKIYQ 130 140 150 160 170 180 RPFGGMTTEY GKGKAAQRTC AAADRTGHAI LHTLYQQSLK HKVQFFVEYF AIDLLMEDGE 190 200 210 220 230 240 CRGVVAWNLD DGSLHCFRAH NVVLATGGYG RAYFSATSAH TCTGDGGGMA IRAGLPLQDM 250 260 270 280 290 300 EFVQFHPTGI YSAGCLITEG ARGEGGYLVN ANGERFMERY APAAKDLASR DVVSRAMTIE 310 320 330 340 350 360 IREGRGVGEH KDHVFLHLNH LSPEILHSRL PGISETAKIF AGVDVTKEPI PVLPTVHYNM 370 380 390 400 410 420 GGIPTNYHGQ VIIKDGKNHN SVVKGLMAIG EAACVSVHGA NRLGSNSLLD LVVFGRSSAL 430 440 450 460 470 480 KAAELIKPAS PHRSIKEESL EKIINRFDKV RHANGNILVA ELRLKMQRTM QSHASVFRTQ 490 500 510 520 530 540 EVLDEGAGMI SEIRSGYKDI KINDKSLIWN SDLVEALELD NLLDQALVTV YSAAARKESR 550 560 570 580 590 GAHAREDYPD RNDEDWMKHT LSSIDEAGKV VLDYKPVTLT TLTDEVTAVP PVKRVY

References

[1]

"Mechanisms of evolution in Rickettsia conorii and R. prowazekii." Ogata H., Audic S., Renesto-Audiffren P., Fournier P.-E., Barbe V., Samson D., Roux V., Cossart P., Weissenbach J., Claverie J.-M., Raoult D. Science 293:2093-2098(2001) [PubMed: 11557893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-613 / Malish 7.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE008583 Genomic DNA. Translation: AAL02708.1.
PIR	B97721.
RefSeq	NP_359807.1.
3D structure databases
HSSP	HSSP built from PDB template 1NEK based on UniProtKB P10444.
ModBase	Search...
Genome annotation databases
GeneID	928018.
GenomeReviews	Gene locus RC0170 in contig AE006914_GR.
KEGG	rco:RC0170.
NMPDR	fig|272944.1.peg.170.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q92J97.
Enzyme and pathway databases
BioCyc	RCON272944:RC0170-MON.
Family and domain databases
InterPro	IPR003953. FAD_bind2_N. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR003952. FRD_SDH_FAD_BS. IPR004112. Fum_Rdtase/Succ_DHase_flav_C. IPR011281. Succ_DHase_flav_su_fwd. IPR014006. Succ_Dhase_frdA_Gneg. [Graphical view]
Pfam	PF00890. FAD_binding_2. 1 hit. PF02910. Succ_DH_flav_C. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR.
TIGRFAMs	TIGR01816. sdhA_forward. 1 hit. TIGR01812. sdhA_frdA_Gneg. 1 hit.
PROSITE	PS00504. FRD_SDH_FAD_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHSA_RICCN

Accession

Primary (citable) accession number: Q92J97

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 11, 2002
Last sequence update:	December 1, 2001
Last modified:	November 25, 2008
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)