Q92IZ6 (GYRA_RICCN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA gyrase subunit A
EC=5.99.1.3

Gene names

Name:	gyrA
Ordered Locus Names:	RC0273

Organism

Rickettsia conorii (strain ATCC VR-613 / Malish 7) [Complete proteome] [HAMAP]

Taxonomic identifier

272944 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	905 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity. HAMAP MF_01897
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP MF_01897
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer By similarity.
Subcellular location	Cytoplasm Potential HAMAP MF_01897.
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 905	905	DNA gyrase subunit A HAMAP MF_01897		PRO_0000145246
Sites
	Active site	123	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q92IZ6 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: CBB5543B3772DA53	FASTA	905	100,701
	10 20 30 40 50 60 MTDKSSSNLV PVNIEDEMKV SYLDYAMSVI VSRAIPDVRD GLKPVHRRII YSMHEAGNHA 70 80 90 100 110 120 SKPYRKSARI VGDVMGKYHP HGDSAIYDAL VRMAQDFSLR LPLVDGQGNF GSMDGDAAAA 130 140 150 160 170 180 MRYTESRMAK VSYKLVEDID KETVSFNPNY DGSEEEPSVL PAMFPNLLVN GSGGIAVGMA 190 200 210 220 230 240 TNIPPHNLGE VIDACCLYID NNDIEILDLL EVVKGPDFPT GSMILGISGI RSAYLTGRGS 250 260 270 280 290 300 IIMRGRAEIE NIGNNRQAII ITEIPYMVNK ARLVEKIAEM VKEKRIEGIS DLRDESNKNG 310 320 330 340 350 360 VRIFIELKKD VVAEVVLNQI YACTQLQTSF GVIMLALKDG LPKVMNLKEV IAAFVSFREV 370 380 390 400 410 420 VITNRTIYLL NKARDRAHIL LGLTIAVSNI DEIIRIIKAS NDSNAAKQEL MARQWEALNI 430 440 450 460 470 480 LPLVKLVDDK AMLNEQGKCN FTEVQAKAIL EMRLQRLTAM EKNKLEEDLK NLITEITEYL 490 500 510 520 530 540 NILGSRTRLL EILKEELIKV KEEFATPRLT SIEFGEFDQD IEDLIQREEM VVTVTLGGYI 550 560 570 580 590 600 KRVPLSSYRA QKRGGKGRSG LSMRDEDITT QVFVGSTHTP MLFFSNIGQV YSLKLYKLPL 610 620 630 640 650 660 SNPQGKGRPM VNILPLKANE HITNIMPLPE NQDERDNLNI MFATAKGNIR RSDLLDFKKL 670 680 690 700 710 720 QSNGKIAIRL DEDDKLIDVK PCKEDEHILL ATKAGKALRF PVESLRVIKS RTSDGVRGMK 730 740 750 760 770 780 LAKEDSVISM TVLKGISSTK EDRDAYLSVP WEQRLEIAKG EAFNPEELGV SLTAPAIVEM 790 800 810 820 830 840 ANSEEFILTV TENGFGKRSS AYGYRITDRG GSGIINMDIN DKTGLVVGVM PVKMDDELML 850 860 870 880 890 900 ITNSGKLIRC KLESVRITGR NTSGVILFKL DDGEKVVSVS LIAETAESEE DSELEEEGLE QSEEV

References

[1]	"Mechanisms of evolution in Rickettsia conorii and R. prowazekii." Ogata H., Audic S., Renesto-Audiffren P., Fournier P.-E., Barbe V., Samson D., Roux V., Cossart P., Weissenbach J., Claverie J.-M., Raoult D. Science 293:2093-2098(2001) [PubMed: 11557893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-613 / Malish 7.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006914 Genomic DNA. Translation: AAL02811.1.
PIR	A97734.
RefSeq	NP_359910.1. NC_003103.1.
3D structure databases
ProteinModelPortal	Q92IZ6.
SMR	Q92IZ6. Positions 31-512.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	927904.
GenomeReviews	Gene locus RC0273 in contig AE006914_GR.
KEGG	rco:RC0273.
NMPDR	fig|272944.1.peg.273.
PATRIC	17887701. VBIRicCon45613_0310.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	TGRGRIY.
ProtClustDB	PRK05560.
Enzyme and pathway databases
BioCyc	RCON272944:RC0273-MONOMER.
Family and domain databases
HAMAP	MF_01897. GyrA. [Tree]
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. IPR013760. Topo_IIA_cen. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
KO	K02469.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01063. GyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GYRA_RICCN

Accession

Primary (citable) accession number: Q92IZ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 11, 2002
Last sequence update:	December 1, 2001
Last modified:	January 25, 2012
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program