Reviewed, UniProtKB/Swiss-Prot Q92IZ1 (DEF1_RICCN)

Names and origin

Protein names

Recommended name:
    Peptide deformylase 1
      Short name=PDF 1
    EC=3.5.1.88
Alternative name(s):
    Polypeptide deformylase 1

Gene names

Name:	def1
Ordered Locus Names:	RC0278

Organism

Rickettsia conorii [Complete proteome] [HAMAP]

Taxonomic identifier

781 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	175 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions By similarity.
Catalytic activity	Formyl-L-methionyl peptide + H2O = formate + methionyl peptide. HAMAP MF_00163
Cofactor	Binds 1 Fe2+ ion By similarity.
Sequence similarities	Belongs to the polypeptide deformylase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	Iron Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW peptide deformylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 175	175	Peptide deformylase 1 HAMAP MF_00163		PRO_0000082829
Sites
	Active site	142	1	By similarity
	Metal binding	99	1	Iron By similarity
	Metal binding	141	1	Iron By similarity
	Metal binding	145	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q92IZ1-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 93B050C6003C5A6D	FASTA	175	20,280
	10 20 30 40 50 60 MSILPIVTAP DERLKQKSQP VLEFTDQTRK FMDDMLKTMY HEDGAGLAAV QVGVLKRILV 70 80 90 100 110 120 IDIQDHDSVA RPKDFYPLFI VNPEIIEKAE ELVTANEGCI SLPEQRIEVA RPESIKIRYL 130 140 150 160 170 DYHGKSRELK ANDWLARVIQ HEYDHLEGKL MIDYLSNLKR DVVLRKLKKL KNNIV

References

[1]

"Mechanisms of evolution in Rickettsia conorii and R. prowazekii." Ogata H., Audic S., Renesto-Audiffren P., Fournier P.-E., Barbe V., Samson D., Roux V., Cossart P., Weissenbach J., Claverie J.-M., Raoult D. Science 293:2093-2098(2001) [PubMed: 11557893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-613 / Malish 7.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE006914 Genomic DNA. Translation: AAL02816.1.
PIR	F97734.
RefSeq	NP_359915.1.
3D structure databases
HSSP	HSSP built from PDB template 1DEF based on UniProtKB P27251.
ModBase	Search...
Genome annotation databases
GeneID	927899.
GenomeReviews	Gene locus RC0278 in contig AE006914_GR.
KEGG	rco:RC0278.
NMPDR	fig|272944.1.peg.278.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q92IZ1.
OMA	LETMYNA.
Enzyme and pathway databases
BioCyc	RCON272944:RC0278-MON.
BRENDA	3.5.1.88. 267518.
Family and domain databases
HAMAP	MF_00163. [Tree]
InterPro	IPR000181. Fmet_deformylase. [Graphical view]
Gene3D	G3DSA:3.90.45.10. Fmet_deformylase. 1 hit.
PANTHER	PTHR10458. Fmet_deformylase. 1 hit.
Pfam	PF01327. Pep_deformylase. 1 hit. [Graphical view]
PIRSF	PIRSF004749. Pep_def. 1 hit.
PRINTS	PR01576. PDEFORMYLASE.
ProDom	PD003844. Fmet_deformylase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00079. pept_deformyl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DEF1_RICCN

Accession

Primary (citable) accession number: Q92IZ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 31, 2002
Last sequence update:	December 1, 2001
Last modified:	November 3, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)