Q92IX4 (SYT_RICCN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Threonine--tRNA ligase
EC=6.1.1.3

Alternative name(s):
Threonyl-tRNA synthetase
Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	RC0296

Organism

Rickettsia conorii (strain ATCC VR-613 / Malish 7) [Complete proteome] [HAMAP]

Taxonomic identifier

272944 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group › Rickettsia conorii

Protein attributes

Sequence length	635 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP-Rule MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW threonine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 635	635	Threonine--tRNA ligase HAMAP-Rule MF_00184		PRO_0000101036
Regions
	Region	242 – 533	292	Catalytic HAMAP-Rule MF_00184
Sites
	Metal binding	333	1	Zinc; catalytic By similarity
	Metal binding	384	1	Zinc; catalytic By similarity
	Metal binding	510	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q92IX4 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: BCF988D88C5CC3EB	FASTA	635	72,353
	10 20 30 40 50 60 MINISFPDGS IKQFAKNITA YEVANAISMS LAKAAMVAEI NGELQDLSIV IDNDCKLRIL 70 80 90 100 110 120 TAQDPECLEI IRHDAAHLTA EAVKELFPET QVTIGPAIEN GYYYDFARDK PFTTDDLAVI 130 140 150 160 170 180 EAKMQELSQK NEQVTRELWD RDKAVEFFKS IGEHYKAEII ASIPAGEPIT LYRQGNFIDL 190 200 210 220 230 240 CRGPHAPSTG VVKHFKLMKV AGAYWRGDSR NEMLQRIYGT AWATKEQLDS YLLMLEEAEK 250 260 270 280 290 300 RDHRKLGREL DLFHFQEEAQ GMVFWHDKGW SIYNTIEQYI RKKIRKNGYT EVKTPVLVDK 310 320 330 340 350 360 SLWEASGHWE KFRDDMFALE TDDKTLALKP MNCPCHVQIF KQGIKSYRDL PLRMSEFGLC 370 380 390 400 410 420 HRNEASGALH GLMRVRSLVQ DDAHIFCAAE QITDETVSFC KLLTEVYKDF GFTDIKVKFS 430 440 450 460 470 480 DRPEIRAGSN EVWDKAENAL KEAVEQAGFN YTLNPGEGAF YGPKLEFVLT DAIGRQWQCG 490 500 510 520 530 540 TLQMDFVLPE RLDASYVAAS GEKKRPVMLH RAILGSLERF IGILIEEYAG RFPLWLAPVQ 550 560 570 580 590 600 VAIATITSDL NDYALEVQKA LIDNGVRTDF NISPDKINYK IREFSNQKIP MIAVIGKQEQ 610 620 630 ENKQVAIRRL GTTDQEVLSV EQLIAVVKEE NEKYL

References

[1]

"Mechanisms of evolution in Rickettsia conorii and R. prowazekii." Ogata H., Audic S., Renesto-Audiffren P., Fournier P.-E., Barbe V., Samson D., Roux V., Cossart P., Weissenbach J., Claverie J.-M., Raoult D. Science 293:2093-2098(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-613 / Malish 7.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006914 Genomic DNA. Translation: AAL02834.1.
PIR	H97736.
RefSeq	NP_359933.1. NC_003103.1.
3D structure databases
ProteinModelPortal	Q92IX4.
SMR	Q92IX4. Positions 241-631.
ModBase	Search...
Protein-protein interaction databases
STRING	272944.RC0296.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL02834; AAL02834; RC0296.
GeneID	927857.
KEGG	rco:RC0296.
PATRIC	17887760. VBIRicCon45613_0337.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0441.
HOGENOM	HOG000003880.
KO	K01868.
OMA	KITYKIR.
ProtClustDB	PRK00413.
Family and domain databases
Gene3D	3.10.20.30. 1 hit. 3.40.50.800. 1 hit.
HAMAP	MF_00184. Thr_tRNA_synth.
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR012675. Beta-grasp_dom. IPR004095. TGS. IPR012676. TGS-like. IPR002320. Thr-tRNA-ligase_IIa. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF03129. HGTP_anticodon. 1 hit. PF02824. TGS. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF81271. TGS-like. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00418. thrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_RICCN

Accession

Primary (citable) accession number: Q92IX4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program