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Protein

DNA topoisomerase 4 subunit B

Gene

parE

Organism
Rickettsia conorii (strain ATCC VR-613 / Malish 7)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule.UniRule annotation

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotation, Ca2+UniRule annotationNote: Binds two Mg2+ per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn2+ or Ca2+.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei20ATPUniRule annotation1
Binding sitei60ATPUniRule annotation1
Binding sitei87ATPUniRule annotation1
Binding sitei359ATPUniRule annotation1
Metal bindingi445Magnesium 1; catalyticUniRule annotation1
Metal bindingi518Magnesium 1; catalyticUniRule annotation1
Metal bindingi518Magnesium 2UniRule annotation1
Metal bindingi520Magnesium 2UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi129 – 135ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

ATP-binding, DNA-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 4 subunit BUniRule annotation (EC:5.99.1.3UniRule annotation)
Alternative name(s):
Topoisomerase IV subunit BUniRule annotation
Gene namesi
Name:parEUniRule annotation
Ordered Locus Names:RC0309
OrganismiRickettsia conorii (strain ATCC VR-613 / Malish 7)
Taxonomic identifieri272944 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
Proteomesi
  • UP000000816 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002731171 – 662DNA topoisomerase 4 subunit BAdd BLAST662

Interactioni

Subunit structurei

Heterotetramer composed of ParC and ParE.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei470Interaction with DNAUniRule annotation1
Sitei473Interaction with DNAUniRule annotation1
Sitei525Interaction with DNAUniRule annotation1
Sitei641Interaction with DNAUniRule annotation1

Structurei

3D structure databases

ProteinModelPortaliQ92IW1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini439 – 553ToprimUniRule annotationAdd BLAST115

Sequence similaritiesi

Belongs to the type II topoisomerase family. ParE type 1 subfamily.UniRule annotation
Contains 1 Toprim domain.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000075154.
KOiK02622.
OMAiTPDRIKL.
OrthoDBiPOG091H03L6.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
HAMAPiMF_00938. ParE_type1. 1 hit.
InterProiIPR002288. DNA_gyrase_B_C.
IPR003594. HATPase_C.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR013760. Topo_IIA-like_dom.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR018522. TopoIIA_CS.
IPR005737. TopoIV_B_Gneg.
IPR006171. Toprim_domain.
[Graphical view]
PfamiPF00204. DNA_gyraseB. 1 hit.
PF00986. DNA_gyraseB_C. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00418. TPI2FAMILY.
SMARTiSM00387. HATPase_c. 1 hit.
SM00433. TOP2c. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 1 hit.
TIGRFAMsiTIGR01055. parE_Gneg. 1 hit.
PROSITEiPS00177. TOPOISOMERASE_II. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q92IW1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDLFSFNKE KKNKLVDNNY SAKDIEVLEG LEPVRKRPGM YIGGTDSNAM
60 70 80 90 100
HHLVSEVLDN AMDEAVAGFA SIITITMHHD HSITIFDNGR GIPIDNHPKF
110 120 130 140 150
PDKSALEVIL TTLHSGGKFS NNVYHTAGGL HGVGISVVNA LSKHLEIKVY
160 170 180 190 200
KQGKLYSQSY SKGEKLTDLI CEEASKRLRG TSINFTPDPE IFSEKLHFNP
210 220 230 240 250
KKIYELARSK AYLYRGVTIE WACEVEVQSD IPKKALISFP NGLKDYLSSK
260 270 280 290 300
ITLDNLVIPG IFAGNIESKS DRIKLEWAIC WQNNDSSAFV QSYCNTVPTP
310 320 330 340 350
QGGTHEQGLK SAILRGLKAY GEMIGNKKAA NLTIEDILET ASVVLSIFIA
360 370 380 390 400
EPSFQGQTKE KLVSNGVSKP VENIIKDHFD HFLSSDKALA TNLLEHVISI
410 420 430 440 450
AEFRISKKNE KNISRKNATQ KLRLPGKLAD CTRTSPEGTE LFIVEGDSAG
460 470 480 490 500
GSAKQARNRE TQAVLPLWGK VLNVASSTLE KIVNNQAIQD LEIALACGSL
510 520 530 540 550
KNYKKENLRY EKIIIMTDAD VDGAHIASLL MTFFFLRMPK LVEEGHLYLA
560 570 580 590 600
KPPLYRLTQS NKTYYAGDEE EKAKLMDKLL KASKAKIEVG RFKGLGEMMP
610 620 630 640 650
AQLKETTMHP EKRSLLKVTL ADFQNVDKIV DDLMGKKPEK RFQFIYEQAL
660
VKMDKIISEL DI
Length:662
Mass (Da):73,827
Last modified:December 1, 2001 - v1
Checksum:iD84CB42C32308088
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006914 Genomic DNA. Translation: AAL02847.1.
PIRiE97738.
RefSeqiWP_010976968.1. NC_003103.1.

Genome annotation databases

EnsemblBacteriaiAAL02847; AAL02847; RC0309.
GeneIDi928839.
KEGGirco:RC0309.
PATRICi17887794. VBIRicCon45613_0354.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006914 Genomic DNA. Translation: AAL02847.1.
PIRiE97738.
RefSeqiWP_010976968.1. NC_003103.1.

3D structure databases

ProteinModelPortaliQ92IW1.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL02847; AAL02847; RC0309.
GeneIDi928839.
KEGGirco:RC0309.
PATRICi17887794. VBIRicCon45613_0354.

Phylogenomic databases

HOGENOMiHOG000075154.
KOiK02622.
OMAiTPDRIKL.
OrthoDBiPOG091H03L6.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
HAMAPiMF_00938. ParE_type1. 1 hit.
InterProiIPR002288. DNA_gyrase_B_C.
IPR003594. HATPase_C.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR013760. Topo_IIA-like_dom.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR018522. TopoIIA_CS.
IPR005737. TopoIV_B_Gneg.
IPR006171. Toprim_domain.
[Graphical view]
PfamiPF00204. DNA_gyraseB. 1 hit.
PF00986. DNA_gyraseB_C. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00418. TPI2FAMILY.
SMARTiSM00387. HATPase_c. 1 hit.
SM00433. TOP2c. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 1 hit.
TIGRFAMsiTIGR01055. parE_Gneg. 1 hit.
PROSITEiPS00177. TOPOISOMERASE_II. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPARE_RICCN
AccessioniPrimary (citable) accession number: Q92IW1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: December 1, 2001
Last modified: September 7, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Rickettsia conorii
    (strain Malish 7): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.