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Protein

DNA topoisomerase 4 subunit B

Gene

parE

Organism
Rickettsia conorii (strain ATCC VR-613 / Malish 7)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule.UniRule annotation

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotation, Ca2+UniRule annotationNote: Binds two Mg(2+) per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn(2+) or Ca2+.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei20 – 201ATPUniRule annotation
Binding sitei60 – 601ATPUniRule annotation
Binding sitei87 – 871ATPUniRule annotation
Binding sitei359 – 3591ATPUniRule annotation
Metal bindingi445 – 4451Magnesium 1; catalyticUniRule annotation
Sitei470 – 4701Interaction with DNAUniRule annotation
Sitei473 – 4731Interaction with DNAUniRule annotation
Metal bindingi518 – 5181Magnesium 1; catalyticUniRule annotation
Metal bindingi518 – 5181Magnesium 2UniRule annotation
Metal bindingi520 – 5201Magnesium 2UniRule annotation
Sitei525 – 5251Interaction with DNAUniRule annotation
Sitei641 – 6411Interaction with DNAUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi129 – 1357ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. DNA binding Source: UniProtKB-HAMAP
  3. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. chromosome segregation Source: UniProtKB-HAMAP
  2. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

ATP-binding, DNA-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 4 subunit BUniRule annotation (EC:5.99.1.3UniRule annotation)
Alternative name(s):
Topoisomerase IV subunit BUniRule annotation
Gene namesi
Name:parEUniRule annotation
Ordered Locus Names:RC0309
OrganismiRickettsia conorii (strain ATCC VR-613 / Malish 7)
Taxonomic identifieri272944 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000000816 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. chromosome Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 662662DNA topoisomerase 4 subunit BPRO_0000273117Add
BLAST

Interactioni

Subunit structurei

Heterotetramer composed of ParC and ParE.UniRule annotation

Protein-protein interaction databases

STRINGi272944.RC0309.

Structurei

3D structure databases

ProteinModelPortaliQ92IW1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini439 – 553115ToprimUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the type II topoisomerase family. ParE type 1 subfamily.UniRule annotation
Contains 1 Toprim domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0187.
HOGENOMiHOG000075154.
KOiK02622.
OMAiLICEEAP.
OrthoDBiEOG6P334W.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
HAMAPiMF_00938. ParE_type1.
InterProiIPR002288. DNA_gyrase_B_C.
IPR003594. HATPase_C.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR013760. Topo_IIA_like_dom.
IPR018522. TopoIIA_CS.
IPR005737. TopoIV_B_Gneg.
IPR006171. Toprim_domain.
[Graphical view]
PfamiPF00204. DNA_gyraseB. 1 hit.
PF00986. DNA_gyraseB_C. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00418. TPI2FAMILY.
SMARTiSM00387. HATPase_c. 1 hit.
SM00433. TOP2c. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 1 hit.
TIGRFAMsiTIGR01055. parE_Gneg. 1 hit.
PROSITEiPS00177. TOPOISOMERASE_II. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q92IW1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDLFSFNKE KKNKLVDNNY SAKDIEVLEG LEPVRKRPGM YIGGTDSNAM
60 70 80 90 100
HHLVSEVLDN AMDEAVAGFA SIITITMHHD HSITIFDNGR GIPIDNHPKF
110 120 130 140 150
PDKSALEVIL TTLHSGGKFS NNVYHTAGGL HGVGISVVNA LSKHLEIKVY
160 170 180 190 200
KQGKLYSQSY SKGEKLTDLI CEEASKRLRG TSINFTPDPE IFSEKLHFNP
210 220 230 240 250
KKIYELARSK AYLYRGVTIE WACEVEVQSD IPKKALISFP NGLKDYLSSK
260 270 280 290 300
ITLDNLVIPG IFAGNIESKS DRIKLEWAIC WQNNDSSAFV QSYCNTVPTP
310 320 330 340 350
QGGTHEQGLK SAILRGLKAY GEMIGNKKAA NLTIEDILET ASVVLSIFIA
360 370 380 390 400
EPSFQGQTKE KLVSNGVSKP VENIIKDHFD HFLSSDKALA TNLLEHVISI
410 420 430 440 450
AEFRISKKNE KNISRKNATQ KLRLPGKLAD CTRTSPEGTE LFIVEGDSAG
460 470 480 490 500
GSAKQARNRE TQAVLPLWGK VLNVASSTLE KIVNNQAIQD LEIALACGSL
510 520 530 540 550
KNYKKENLRY EKIIIMTDAD VDGAHIASLL MTFFFLRMPK LVEEGHLYLA
560 570 580 590 600
KPPLYRLTQS NKTYYAGDEE EKAKLMDKLL KASKAKIEVG RFKGLGEMMP
610 620 630 640 650
AQLKETTMHP EKRSLLKVTL ADFQNVDKIV DDLMGKKPEK RFQFIYEQAL
660
VKMDKIISEL DI
Length:662
Mass (Da):73,827
Last modified:December 1, 2001 - v1
Checksum:iD84CB42C32308088
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006914 Genomic DNA. Translation: AAL02847.1.
PIRiE97738.
RefSeqiNP_359946.1. NC_003103.1.

Genome annotation databases

EnsemblBacteriaiAAL02847; AAL02847; RC0309.
KEGGirco:RC0309.
PATRICi17887794. VBIRicCon45613_0354.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006914 Genomic DNA. Translation: AAL02847.1.
PIRiE97738.
RefSeqiNP_359946.1. NC_003103.1.

3D structure databases

ProteinModelPortaliQ92IW1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272944.RC0309.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL02847; AAL02847; RC0309.
KEGGirco:RC0309.
PATRICi17887794. VBIRicCon45613_0354.

Phylogenomic databases

eggNOGiCOG0187.
HOGENOMiHOG000075154.
KOiK02622.
OMAiLICEEAP.
OrthoDBiEOG6P334W.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
HAMAPiMF_00938. ParE_type1.
InterProiIPR002288. DNA_gyrase_B_C.
IPR003594. HATPase_C.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR013760. Topo_IIA_like_dom.
IPR018522. TopoIIA_CS.
IPR005737. TopoIV_B_Gneg.
IPR006171. Toprim_domain.
[Graphical view]
PfamiPF00204. DNA_gyraseB. 1 hit.
PF00986. DNA_gyraseB_C. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00418. TPI2FAMILY.
SMARTiSM00387. HATPase_c. 1 hit.
SM00433. TOP2c. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 1 hit.
TIGRFAMsiTIGR01055. parE_Gneg. 1 hit.
PROSITEiPS00177. TOPOISOMERASE_II. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC VR-613 / Malish 7.

Entry informationi

Entry nameiPARE_RICCN
AccessioniPrimary (citable) accession number: Q92IW1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: December 1, 2001
Last modified: April 1, 2015
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Rickettsia conorii
    (strain Malish 7): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.