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Q92IL0

- MNMA_RICCN

UniProt

Q92IL0 - MNMA_RICCN

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Protein

tRNA-specific 2-thiouridylase MnmA

Gene

mnmA

Organism
Rickettsia conorii (strain ATCC VR-613 / Malish 7)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s2U34.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei45 – 451ATP; via amide nitrogen and carbonyl oxygenUniRule annotation
Active sitei113 – 1131NucleophileUniRule annotation
Binding sitei137 – 1371ATP; via amide nitrogenUniRule annotation
Sitei138 – 1381Interaction with tRNAUniRule annotation
Active sitei209 – 2091Cysteine persulfide intermediateUniRule annotation
Sitei349 – 3491Interaction with tRNAUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 268ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. sulfurtransferase activity Source: InterPro
  3. tRNA binding Source: UniProtKB-KW

GO - Biological processi

  1. tRNA modification Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding, tRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-specific 2-thiouridylase MnmAUniRule annotation (EC:2.8.1.-UniRule annotation)
Gene namesi
Name:mnmAUniRule annotation
Synonyms:trmU
Ordered Locus Names:RC0410
OrganismiRickettsia conorii (strain ATCC VR-613 / Malish 7)
Taxonomic identifieri272944 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000000816: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 370370tRNA-specific 2-thiouridylase MnmAPRO_0000121669Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi113 ↔ 209AlternateUniRule annotation

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

STRINGi272944.RC0410.

Structurei

3D structure databases

ProteinModelPortaliQ92IL0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni159 – 1613Interaction with tRNAUniRule annotation

Sequence similaritiesi

Belongs to the MnmA/TRMU family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0482.
HOGENOMiHOG000218045.
KOiK00566.
OMAiFEHFLEE.
OrthoDBiEOG6RZB5H.

Family and domain databases

Gene3Di2.30.30.280. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_00144. tRNA_thiouridyl_MnmA.
InterProiIPR023382. Adenine_a_hdrlase_dom.
IPR014729. Rossmann-like_a/b/a_fold.
IPR004506. tRNA-specific_2-thiouridylase.
[Graphical view]
PANTHERiPTHR11933. PTHR11933. 1 hit.
PfamiPF03054. tRNA_Me_trans. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00420. trmU. 1 hit.

Sequencei

Sequence statusi: Complete.

Q92IL0-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MFKVGMINLG DKQSTIVVAM SGGVDSSAVA AMLHEQGHNV IGITLQLYDH
60 70 80 90 100
GMAVGKKNAC CAGQDIYDAK MVANKLGIPH YVLDYENKFK ESVIDNFVDS
110 120 130 140 150
YLQGETPLPC VQCNKSVKFR DLIKTARELG ADKLATGHYV RKINGDNGAE
160 170 180 190 200
LHTGLDPAKD QSYFLFTTTK EQLEYLRFPL GGLTKDETRK LASKFGLEVA
210 220 230 240 250
DKPDSQDICF IPDGNYKSVI NKIRPNSSES GKIIHVNGFE LGEHSGIINY
260 270 280 290 300
TIGQRRGLGI AYNEPLYVVK IDPKDNIVYV GPESALNVQE FIIRDVNWLA
310 320 330 340 350
DEIKDNEKLE VAVKIRSTRP PRLAEISKLG DDKMKVKFLC EEKAVAPGQA
360 370
CVIYAGERVL GGGWITREIR
Length:370
Mass (Da):40,915
Last modified:December 1, 2001 - v1
Checksum:i1884AC815E730CE3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006914 Genomic DNA. Translation: AAL02948.1.
PIRiB97751.
RefSeqiNP_360047.1. NC_003103.1.
WP_010977062.1. NC_003103.1.

Genome annotation databases

EnsemblBacteriaiAAL02948; AAL02948; RC0410.
GeneIDi927560.
KEGGirco:RC0410.
PATRICi17888019. VBIRicCon45613_0465.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006914 Genomic DNA. Translation: AAL02948.1 .
PIRi B97751.
RefSeqi NP_360047.1. NC_003103.1.
WP_010977062.1. NC_003103.1.

3D structure databases

ProteinModelPortali Q92IL0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272944.RC0410.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL02948 ; AAL02948 ; RC0410 .
GeneIDi 927560.
KEGGi rco:RC0410.
PATRICi 17888019. VBIRicCon45613_0465.

Phylogenomic databases

eggNOGi COG0482.
HOGENOMi HOG000218045.
KOi K00566.
OMAi FEHFLEE.
OrthoDBi EOG6RZB5H.

Family and domain databases

Gene3Di 2.30.30.280. 1 hit.
3.40.50.620. 1 hit.
HAMAPi MF_00144. tRNA_thiouridyl_MnmA.
InterProi IPR023382. Adenine_a_hdrlase_dom.
IPR014729. Rossmann-like_a/b/a_fold.
IPR004506. tRNA-specific_2-thiouridylase.
[Graphical view ]
PANTHERi PTHR11933. PTHR11933. 1 hit.
Pfami PF03054. tRNA_Me_trans. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00420. trmU. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC VR-613 / Malish 7.

Entry informationi

Entry nameiMNMA_RICCN
AccessioniPrimary (citable) accession number: Q92IL0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: December 1, 2001
Last modified: October 29, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Rickettsia conorii
    (strain Malish 7): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3