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Q92IL0 (MNMA_RICCN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA-specific 2-thiouridylase MnmA
EC=2.8.1.-
Gene names
Name:mnmA
Synonyms:trmU
Ordered Locus Names:RC0410
OrganismRickettsia conorii (strain ATCC VR-613 / Malish 7) [Complete proteome] [HAMAP]
Taxonomic identifier272944 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group ›

Protein attributes

Sequence length370 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s2U34 By similarity. HAMAP-Rule MF_00144

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the MnmA/TRMU family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
RNA-binding
tRNA-binding
   Molecular functionTransferase
   PTMDisulfide bond
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtRNA modification

Inferred from electronic annotation. Source: HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

sulfurtransferase activity

Inferred from electronic annotation. Source: InterPro

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 370370tRNA-specific 2-thiouridylase MnmA HAMAP-Rule MF_00144
PRO_0000121669

Regions

Nucleotide binding19 – 268ATP By similarity
Region159 – 1613Interaction with tRNA By similarity

Sites

Active site1131Nucleophile By similarity
Active site2091Cysteine persulfide intermediate By similarity
Binding site451ATP; via amide nitrogen and carbonyl oxygen By similarity
Binding site1371ATP; via amide nitrogen By similarity
Site1381Interaction with tRNA By similarity
Site3491Interaction with tRNA By similarity

Amino acid modifications

Disulfide bond113 ↔ 209Alternate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q92IL0 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 1884AC815E730CE3

FASTA37040,915
        10         20         30         40         50         60 
MFKVGMINLG DKQSTIVVAM SGGVDSSAVA AMLHEQGHNV IGITLQLYDH GMAVGKKNAC 

        70         80         90        100        110        120 
CAGQDIYDAK MVANKLGIPH YVLDYENKFK ESVIDNFVDS YLQGETPLPC VQCNKSVKFR 

       130        140        150        160        170        180 
DLIKTARELG ADKLATGHYV RKINGDNGAE LHTGLDPAKD QSYFLFTTTK EQLEYLRFPL 

       190        200        210        220        230        240 
GGLTKDETRK LASKFGLEVA DKPDSQDICF IPDGNYKSVI NKIRPNSSES GKIIHVNGFE 

       250        260        270        280        290        300 
LGEHSGIINY TIGQRRGLGI AYNEPLYVVK IDPKDNIVYV GPESALNVQE FIIRDVNWLA 

       310        320        330        340        350        360 
DEIKDNEKLE VAVKIRSTRP PRLAEISKLG DDKMKVKFLC EEKAVAPGQA CVIYAGERVL 

       370 
GGGWITREIR

« Hide

References

[1]"Mechanisms of evolution in Rickettsia conorii and R. prowazekii."
Ogata H., Audic S., Renesto-Audiffren P., Fournier P.-E., Barbe V., Samson D., Roux V., Cossart P., Weissenbach J., Claverie J.-M., Raoult D.
Science 293:2093-2098(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC VR-613 / Malish 7.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE006914 Genomic DNA. Translation: AAL02948.1.
PIRB97751.
RefSeqNP_360047.1. NC_003103.1.

3D structure databases

ProteinModelPortalQ92IL0.
ModBaseSearch...

Protein-protein interaction databases

STRING272944.RC0410.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL02948; AAL02948; RC0410.
GeneID927560.
KEGGrco:RC0410.
PATRIC17888019. VBIRicCon45613_0465.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0482.
HOGENOMHOG000218045.
KOK00566.
OMAQPGNIET.
ProtClustDBPRK00143.

Family and domain databases

Gene3D2.30.30.280. 1 hit.
3.40.50.620. 1 hit.
HAMAPMF_00144. tRNA_thiouridyl_MnmA.
InterProIPR023382. Adenine_a_hdrlase_dom.
IPR014729. Rossmann-like_a/b/a_fold.
IPR004506. tRNA-specific_2-thiouridylase.
[Graphical view]
PANTHERPTHR11933. PTHR11933. 1 hit.
PfamPF03054. tRNA_Me_trans. 1 hit.
[Graphical view]
TIGRFAMsTIGR00420. trmU. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMNMA_RICCN
AccessionPrimary (citable) accession number: Q92IL0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: December 1, 2001
Last modified: May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Rickettsia conorii

(strain Malish 7): entries and gene names

SIMILARITY comments

Index of protein domains and families

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