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Protein

tRNA-specific 2-thiouridylase MnmA

Gene

mnmA

Organism
Rickettsia conorii (strain ATCC VR-613 / Malish 7)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s2U34.UniRule annotation

Catalytic activityi

A [protein]-S-sulfanyl-L-cysteine + uridine(34) in tRNA + ATP + reduced acceptor = a [protein]-L-cysteine + 2-thiouridine(34) in tRNA + AMP + diphosphate + acceptor.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei45ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1
Active sitei113NucleophileUniRule annotation1
Binding sitei137ATP; via amide nitrogenUniRule annotation1
Active sitei209Cysteine persulfide intermediateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi19 – 26ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding, tRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-specific 2-thiouridylase MnmAUniRule annotation (EC:2.8.1.13UniRule annotation)
Gene namesi
Name:mnmAUniRule annotation
Synonyms:trmU
Ordered Locus Names:RC0410
OrganismiRickettsia conorii (strain ATCC VR-613 / Malish 7)
Taxonomic identifieri272944 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
Proteomesi
  • UP000000816 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001216691 – 370tRNA-specific 2-thiouridylase MnmAAdd BLAST370

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi113 ↔ 209AlternateUniRule annotation

Keywords - PTMi

Disulfide bond

Interactioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei138Interaction with tRNAUniRule annotation1
Sitei349Interaction with tRNAUniRule annotation1

Structurei

3D structure databases

ProteinModelPortaliQ92IL0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni159 – 161Interaction with tRNAUniRule annotation3

Sequence similaritiesi

Belongs to the MnmA/TRMU family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000218045.
KOiK00566.
OMAiCIACNRY.
OrthoDBiPOG091H02F0.

Family and domain databases

CDDicd01998. tRNA_Me_trans. 1 hit.
Gene3Di2.30.30.280. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_00144. tRNA_thiouridyl_MnmA. 1 hit.
InterProiIPR023382. Adenine_a_hdrlase_dom.
IPR014729. Rossmann-like_a/b/a_fold.
IPR004506. tRNA-specific_2-thiouridylase.
[Graphical view]
PANTHERiPTHR11933. PTHR11933. 1 hit.
TIGRFAMsiTIGR00420. trmU. 1 hit.

Sequencei

Sequence statusi: Complete.

Q92IL0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKVGMINLG DKQSTIVVAM SGGVDSSAVA AMLHEQGHNV IGITLQLYDH
60 70 80 90 100
GMAVGKKNAC CAGQDIYDAK MVANKLGIPH YVLDYENKFK ESVIDNFVDS
110 120 130 140 150
YLQGETPLPC VQCNKSVKFR DLIKTARELG ADKLATGHYV RKINGDNGAE
160 170 180 190 200
LHTGLDPAKD QSYFLFTTTK EQLEYLRFPL GGLTKDETRK LASKFGLEVA
210 220 230 240 250
DKPDSQDICF IPDGNYKSVI NKIRPNSSES GKIIHVNGFE LGEHSGIINY
260 270 280 290 300
TIGQRRGLGI AYNEPLYVVK IDPKDNIVYV GPESALNVQE FIIRDVNWLA
310 320 330 340 350
DEIKDNEKLE VAVKIRSTRP PRLAEISKLG DDKMKVKFLC EEKAVAPGQA
360 370
CVIYAGERVL GGGWITREIR
Length:370
Mass (Da):40,915
Last modified:December 1, 2001 - v1
Checksum:i1884AC815E730CE3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006914 Genomic DNA. Translation: AAL02948.1.
PIRiB97751.

Genome annotation databases

EnsemblBacteriaiAAL02948; AAL02948; RC0410.
KEGGirco:RC0410.
PATRICi17888019. VBIRicCon45613_0465.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006914 Genomic DNA. Translation: AAL02948.1.
PIRiB97751.

3D structure databases

ProteinModelPortaliQ92IL0.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL02948; AAL02948; RC0410.
KEGGirco:RC0410.
PATRICi17888019. VBIRicCon45613_0465.

Phylogenomic databases

HOGENOMiHOG000218045.
KOiK00566.
OMAiCIACNRY.
OrthoDBiPOG091H02F0.

Family and domain databases

CDDicd01998. tRNA_Me_trans. 1 hit.
Gene3Di2.30.30.280. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_00144. tRNA_thiouridyl_MnmA. 1 hit.
InterProiIPR023382. Adenine_a_hdrlase_dom.
IPR014729. Rossmann-like_a/b/a_fold.
IPR004506. tRNA-specific_2-thiouridylase.
[Graphical view]
PANTHERiPTHR11933. PTHR11933. 1 hit.
TIGRFAMsiTIGR00420. trmU. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMNMA_RICCN
AccessioniPrimary (citable) accession number: Q92IL0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: December 1, 2001
Last modified: October 5, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Rickettsia conorii
    (strain Malish 7): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.