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Q92IL0

- MNMA_RICCN

UniProt

Q92IL0 - MNMA_RICCN

Protein

tRNA-specific 2-thiouridylase MnmA

Gene

mnmA

Organism
Rickettsia conorii (strain ATCC VR-613 / Malish 7)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s2U34.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei45 – 451ATP; via amide nitrogen and carbonyl oxygenUniRule annotation
    Active sitei113 – 1131NucleophileUniRule annotation
    Binding sitei137 – 1371ATP; via amide nitrogenUniRule annotation
    Sitei138 – 1381Interaction with tRNAUniRule annotation
    Active sitei209 – 2091Cysteine persulfide intermediateUniRule annotation
    Sitei349 – 3491Interaction with tRNAUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi19 – 268ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. sulfurtransferase activity Source: InterPro
    3. tRNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. tRNA modification Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, RNA-binding, tRNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA-specific 2-thiouridylase MnmAUniRule annotation (EC:2.8.1.-UniRule annotation)
    Gene namesi
    Name:mnmAUniRule annotation
    Synonyms:trmU
    Ordered Locus Names:RC0410
    OrganismiRickettsia conorii (strain ATCC VR-613 / Malish 7)
    Taxonomic identifieri272944 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
    ProteomesiUP000000816: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 370370tRNA-specific 2-thiouridylase MnmAPRO_0000121669Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi113 ↔ 209AlternateUniRule annotation

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Protein-protein interaction databases

    STRINGi272944.RC0410.

    Structurei

    3D structure databases

    ProteinModelPortaliQ92IL0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni159 – 1613Interaction with tRNAUniRule annotation

    Sequence similaritiesi

    Belongs to the MnmA/TRMU family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0482.
    HOGENOMiHOG000218045.
    KOiK00566.
    OMAiFEHFLEE.
    OrthoDBiEOG6RZB5H.

    Family and domain databases

    Gene3Di2.30.30.280. 1 hit.
    3.40.50.620. 1 hit.
    HAMAPiMF_00144. tRNA_thiouridyl_MnmA.
    InterProiIPR023382. Adenine_a_hdrlase_dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR004506. tRNA-specific_2-thiouridylase.
    [Graphical view]
    PANTHERiPTHR11933. PTHR11933. 1 hit.
    PfamiPF03054. tRNA_Me_trans. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00420. trmU. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q92IL0-1 [UniParc]FASTAAdd to Basket

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    MFKVGMINLG DKQSTIVVAM SGGVDSSAVA AMLHEQGHNV IGITLQLYDH    50
    GMAVGKKNAC CAGQDIYDAK MVANKLGIPH YVLDYENKFK ESVIDNFVDS 100
    YLQGETPLPC VQCNKSVKFR DLIKTARELG ADKLATGHYV RKINGDNGAE 150
    LHTGLDPAKD QSYFLFTTTK EQLEYLRFPL GGLTKDETRK LASKFGLEVA 200
    DKPDSQDICF IPDGNYKSVI NKIRPNSSES GKIIHVNGFE LGEHSGIINY 250
    TIGQRRGLGI AYNEPLYVVK IDPKDNIVYV GPESALNVQE FIIRDVNWLA 300
    DEIKDNEKLE VAVKIRSTRP PRLAEISKLG DDKMKVKFLC EEKAVAPGQA 350
    CVIYAGERVL GGGWITREIR 370
    Length:370
    Mass (Da):40,915
    Last modified:December 1, 2001 - v1
    Checksum:i1884AC815E730CE3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE006914 Genomic DNA. Translation: AAL02948.1.
    PIRiB97751.
    RefSeqiNP_360047.1. NC_003103.1.
    WP_010977062.1. NC_003103.1.

    Genome annotation databases

    EnsemblBacteriaiAAL02948; AAL02948; RC0410.
    GeneIDi927560.
    KEGGirco:RC0410.
    PATRICi17888019. VBIRicCon45613_0465.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE006914 Genomic DNA. Translation: AAL02948.1 .
    PIRi B97751.
    RefSeqi NP_360047.1. NC_003103.1.
    WP_010977062.1. NC_003103.1.

    3D structure databases

    ProteinModelPortali Q92IL0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272944.RC0410.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL02948 ; AAL02948 ; RC0410 .
    GeneIDi 927560.
    KEGGi rco:RC0410.
    PATRICi 17888019. VBIRicCon45613_0465.

    Phylogenomic databases

    eggNOGi COG0482.
    HOGENOMi HOG000218045.
    KOi K00566.
    OMAi FEHFLEE.
    OrthoDBi EOG6RZB5H.

    Family and domain databases

    Gene3Di 2.30.30.280. 1 hit.
    3.40.50.620. 1 hit.
    HAMAPi MF_00144. tRNA_thiouridyl_MnmA.
    InterProi IPR023382. Adenine_a_hdrlase_dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR004506. tRNA-specific_2-thiouridylase.
    [Graphical view ]
    PANTHERi PTHR11933. PTHR11933. 1 hit.
    Pfami PF03054. tRNA_Me_trans. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00420. trmU. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC VR-613 / Malish 7.

    Entry informationi

    Entry nameiMNMA_RICCN
    AccessioniPrimary (citable) accession number: Q92IL0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 2, 2002
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Rickettsia conorii
      (strain Malish 7): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3