ID   LPXB_RICCN              Reviewed;         446 AA.
AC   Q92II0;
DT   27-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   27-MAR-2024, entry version 99.
DE   RecName: Full=Lipid-A-disaccharide synthase;
DE            EC=2.4.1.182;
GN   Name=lpxB; OrderedLocusNames=RC0440;
OS   Rickettsia conorii (strain ATCC VR-613 / Malish 7).
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Rickettsiales;
OC   Rickettsiaceae; Rickettsieae; Rickettsia; spotted fever group.
OX   NCBI_TaxID=272944;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC VR-613 / Malish 7;
RX   PubMed=11557893; DOI=10.1126/science.1061471;
RA   Ogata H., Audic S., Renesto-Audiffren P., Fournier P.-E., Barbe V.,
RA   Samson D., Roux V., Cossart P., Weissenbach J., Claverie J.-M., Raoult D.;
RT   "Mechanisms of evolution in Rickettsia conorii and R. prowazekii.";
RL   Science 293:2093-2098(2001).
CC   -!- FUNCTION: Condensation of UDP-2,3-diacylglucosamine and 2,3-
CC       diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor
CC       of lipid A, a phosphorylated glycolipid that anchors the
CC       lipopolysaccharide to the outer membrane of the cell. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a lipid X + a UDP-2-N,3-O-bis[(3R)-3-hydroxyacyl]-alpha-D-
CC         glucosamine = a lipid A disaccharide + H(+) + UDP;
CC         Xref=Rhea:RHEA:67828, ChEBI:CHEBI:15378, ChEBI:CHEBI:58223,
CC         ChEBI:CHEBI:137748, ChEBI:CHEBI:176338, ChEBI:CHEBI:176343;
CC         EC=2.4.1.182;
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; LPS lipid A biosynthesis.
CC   -!- SIMILARITY: Belongs to the LpxB family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE006914; AAL02978.1; -; Genomic_DNA.
DR   PIR; H97754; H97754.
DR   RefSeq; WP_010977087.1; NC_003103.1.
DR   AlphaFoldDB; Q92II0; -.
DR   SMR; Q92II0; -.
DR   CAZy; GT19; Glycosyltransferase Family 19.
DR   GeneID; 928676; -.
DR   KEGG; rco:RC0440; -.
DR   PATRIC; fig|272944.4.peg.498; -.
DR   HOGENOM; CLU_036577_2_0_5; -.
DR   UniPathway; UPA00973; -.
DR   Proteomes; UP000000816; Chromosome.
DR   GO; GO:0008915; F:lipid-A-disaccharide synthase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009245; P:lipid A biosynthetic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.2000; Glycogen Phosphorylase B; 1.
DR   HAMAP; MF_00392; LpxB; 1.
DR   InterPro; IPR003835; Glyco_trans_19.
DR   InterPro; IPR022437; RPE3.
DR   NCBIfam; TIGR00215; lpxB; 1.
DR   NCBIfam; TIGR03775; RPE3; 1.
DR   PANTHER; PTHR30372; LIPID-A-DISACCHARIDE SYNTHASE; 1.
DR   PANTHER; PTHR30372:SF4; LIPID-A-DISACCHARIDE SYNTHASE, MITOCHONDRIAL-RELATED; 1.
DR   Pfam; PF02684; LpxB; 1.
DR   SUPFAM; SSF53756; UDP-Glycosyltransferase/glycogen phosphorylase; 1.
PE   3: Inferred from homology;
KW   Glycosyltransferase; Lipid A biosynthesis; Lipid biosynthesis;
KW   Lipid metabolism; Transferase.
FT   CHAIN           1..446
FT                   /note="Lipid-A-disaccharide synthase"
FT                   /id="PRO_0000190181"
FT   DOMAIN          379..418
FT                   /note="RPE3 insert"
SQ   SEQUENCE   446 AA;  50738 MW;  FD5F11728238FDB7 CRC64;
     MIKIYFIAGE VSGDFVGGRI MQHLKNNTGV QLNSPVSSFV NDAVQFVGVG GKYMEEAGSF
     KSLFPITSIN LMGFVEILPH IFKLKKLIDK TVEDIINSKA DLLITIDSPG FTYRVAKRVR
     KLLPKLKMIH IVAPSVWAYK EGRAVKYAKI YDCLFALLPF EPPYFTKVGL DCRYIGHPIM
     EQEFYSDKIA LREEFKIDEN ERVLCVTLGS RKGEILRHLS VFVSSIEEIF KSCNNLKVIF
     TLANPAHEAI IKPFLEDVKF NYLFSSERLK IYAVADVALA KSGTNTLEIA ASGTPMIVAY
     KVNLISFFII RLLIKIKYVT LINIIADKEI IPEFIQFNCR ANLISNKLQE LLFNSKKAYE
     QVIESQKILQ QLGFKSNRLQ LNSESFRHDE FKGKLARCTK VREHRLSLEN SLVSSDRDDA
     VPSYIAAAII KQEFLEPKIK LLKEKD
//