Q92IH1 (TOP1_RICCN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA topoisomerase 1
EC=5.99.1.2

Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme

Gene names

Name:	topA
Ordered Locus Names:	RC0449

Organism

Rickettsia conorii (strain ATCC VR-613 / Malish 7) [Complete proteome] [HAMAP]

Taxonomic identifier

272944 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	776 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the type IA topoisomerase family. Contains 1 Toprim domain.

Ontologies

Keywords
Domain	Zinc-finger
Ligand	ATP-binding DNA-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 776	776	DNA topoisomerase 1		PRO_0000145162
Regions
	Domain	1 – 114	114	Toprim
	Zinc finger	600 – 627	28	C4-type
Sites
	Active site	304	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q92IH1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 2EB182D7A4BB68FA	FASTA	776	88,145
	10 20 30 40 50 60 MKLVIVESPA KAKTINKYLG DEFKVIASFG HIRDLPSKKG SVLPDENFAM KYDISDKAGK 70 80 90 100 110 120 YVDAIVKDAK KADAVYLATD PDREGESISW HVAEVIKEKN QVKSDDFFKR VAFNEITKKA 130 140 150 160 170 180 IIHAVENPRK LDTNLVNAQQ ARRALDYLVG FTLSPLLWRK LPGCKSAGRV QSVALRLICE 190 200 210 220 230 240 REDEIERFKS EEYWDISLKM QNSNNELFTA KLTHVNDQKL EKFSIINEKN AKDLTKKLKS 250 260 270 280 290 300 HKFHVDKIEK KQQKRQPQPP FITSSLQQEA ARKLGFSAKK TMQIAQKLYE GVDIGKETIG 310 320 330 340 350 360 LITYMRTDGV TLSNDAIADI RKLIDKSYGD KYLPTSPRIY KSKVKNAQEA HEAIRPTNIT 370 380 390 400 410 420 YTPDSLKEKL DKDYYKLYEL IWKRTIACQM ENVIMDLVVA NLASENKEYL AKANGSTIAF 430 440 450 460 470 480 DGFYKVYRES MDDEAEEENK MLPPLKEQEP LKTKAVIPNQ HFTEPPPRYS EASLVKKLEE 490 500 510 520 530 540 LGIGRPSTYA SILSVLQDRK YVALEKKRFI PEELGRLVTV FLVGFFKKYV EYDFTAGLEN 550 560 570 580 590 600 ELDAIAAGKL EWKAALNNFW SGFNHNIESV NEQKITEIIS YVQKALDYHL FGENKESKIC 610 620 630 640 650 660 PSCNTGELSL KLGKFGAFLA CSNYPECTFR KSIVSGNDNN ENEGEPAAMP NENKVLGTDK 670 680 690 700 710 720 DGVEIYLKKG PYGPYIQLGK QEGKVKPKRS PVPASLNQND ITLDIALKLL SLPLKIGIHK 730 740 750 760 770 DSDEEIMIGY GKFGPYIKYM GQFISVQKKY DFLNLSLDDA MKLIEENKAK LEKKQA

References

[1]	"Mechanisms of evolution in Rickettsia conorii and R. prowazekii." Ogata H., Audic S., Renesto-Audiffren P., Fournier P.-E., Barbe V., Samson D., Roux V., Cossart P., Weissenbach J., Claverie J.-M., Raoult D. Science 293:2093-2098(2001) [PubMed: 11557893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-613 / Malish 7.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006914 Genomic DNA. Translation: AAL02987.1.
PIR	A97756.
RefSeq	NP_360086.1. NC_003103.1.
3D structure databases
ProteinModelPortal	Q92IH1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	927585.
GenomeReviews	Gene locus RC0449 in contig AE006914_GR.
KEGG	rco:RC0449.
NMPDR	fig|272944.1.peg.449.
PATRIC	17888111. VBIRicCon45613_0511.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	EEYWSID.
ProtClustDB	PRK06599.
Enzyme and pathway databases
BioCyc	RCON272944:RC0449-MONOMER.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TOP1_RICCN

Accession

Primary (citable) accession number: Q92IH1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 11, 2002
Last sequence update:	December 1, 2001
Last modified:	January 25, 2012
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program