Reviewed, UniProtKB/Swiss-Prot Q92ID6 (NUOB_RICCN)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit B
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit B
    NDH-1 subunit B

Gene names

Name:	nuoB
Ordered Locus Names:	RC0484

Organism

Rickettsia conorii [Complete proteome] [HAMAP]

Taxonomic identifier

781 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	174 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD(+) + quinol.
Cofactor	Binds 1 4Fe-4S cluster Potential.
Sequence similarities	Belongs to the complex I 20 kDa subunit family.

Ontologies

Keywords
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding NAD
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	mitochondrial electron transport, NADH to ubiquinone Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 174	174	NADH-quinone oxidoreductase subunit B		PRO_0000118778
Sites
	Metal binding	47	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	48	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	112	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	142	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q92ID6-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: CC084518CAECB22B	FASTA	174	19,616
	10 20 30 40 50 60 MKNNFYQEDE LLSNELSNRG FLLTKVDDVI GWARANSLWP MTFGLACCAV EMMQAAASRY 70 80 90 100 110 120 DMDRFGMLFR PSPRQSDLMI VAGTLTNKMA PALRKVYDQM AEPKWVLSMG SCANGGGYYH 130 140 150 160 170 FSYSVVRGCD RIVPVDVYVP GCPPTAEALI YGLMQLQKKI KRTTGFKYDA RQTH

References

[1]

"Mechanisms of evolution in Rickettsia conorii and R. prowazekii." Ogata H., Audic S., Renesto-Audiffren P., Fournier P.-E., Barbe V., Samson D., Roux V., Cossart P., Weissenbach J., Claverie J.-M., Raoult D. Science 293:2093-2098(2001) [PubMed: 11557893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-613 / Malish 7.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE008611 Genomic DNA. Translation: AAL03022.1.
PIR	D97760.
RefSeq	NP_360121.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	927609.
GenomeReviews	Gene locus RC0484 in contig AE006914_GR.
KEGG	rco:RC0484.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q92ID6.
Enzyme and pathway databases
BioCyc	RCON272944:RC0484-MON.
Family and domain databases
InterPro	IPR006138. NADH_DHase_20kDa_su. IPR014406. NiFe_hyd_3_ssu/Q_oxred_NuoB. IPR006137. OxRdtase_q6. [Graphical view]
PANTHER	PTHR11995:SF2. NADH_DH_20kDa. 1 hit. PTHR11995. NiFe_hyd_3_ssu/Q_oxred_NuoB. 1 hit.
Pfam	PF01058. Oxidored_q6. 1 hit. [Graphical view]
TIGRFAMs	TIGR01957. nuoB_fam. 1 hit.
PROSITE	PS01150. COMPLEX1_20K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOB_RICCN

Accession

Primary (citable) accession number: Q92ID6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	December 1, 2001
Last modified:	November 25, 2008
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)