Q92IC6 (FABI_RICCN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Enoyl-[acyl-carrier-protein] reductase [NADH] FabI
Short name=ENR
EC=1.3.1.9

Alternative name(s):
NADH-dependent enoyl-ACP reductase

Gene names

Name:	fabI
Ordered Locus Names:	RC0494

Organism

Rickettsia conorii (strain ATCC VR-613 / Malish 7) [Complete proteome] [HAMAP]

Taxonomic identifier

272944 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Involved in the elongation cycle of fatty acid which are used in the lipid metabolism By similarity.
Catalytic activity	Acyl-[acyl-carrier-protein] + NAD+ = trans-2,3-dehydroacyl-[acyl-carrier-protein] + NADH.
Pathway	Lipid metabolism; fatty acid biosynthesis.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fatty acid elongation Inferred from sequence or structural similarity. Source: UniProtKB protein homotetramerization Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	enoyl-[acyl-carrier-protein] reductase (NADH) activity Inferred from sequence or structural similarity. Source: UniProtKB nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 260	260	Enoyl-[acyl-carrier-protein] reductase [NADH] FabI		PRO_0000054908
Regions
	Nucleotide binding	21 – 22	2	NAD By similarity
	Nucleotide binding	66 – 67	2	NAD By similarity
	Nucleotide binding	193 – 195	3	NAD By similarity
Sites
	Active site	147	1	Proton acceptor By similarity
	Active site	157	1	Proton acceptor By similarity
	Binding site	15	1	NAD; via carbonyl oxygen By similarity
	Binding site	42	1	NAD By similarity
	Binding site	94	1	NAD; via carbonyl oxygen By similarity
	Binding site	97	1	Substrate; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	164	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q92IC6 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: DEA257B68B8FAA46	FASTA	260	28,211
	10 20 30 40 50 60 MTTGLLQGKK GLITGIANNM SISWAIAQLA KKHGAELWFT YQSGVLEKRV KPLAKEIGCN 70 80 90 100 110 120 FVSELDVTKP KSISNLFDDI KEKWGSFDFL LHGMAFADKN ELKGRYVDTS LENFHNSLHI 130 140 150 160 170 180 SCYSLLELSR SAEALMHNGG SIVTLTYYGA EKVIPNYNVM GVAKAALEAS IKYLANDMGE 190 200 210 220 230 240 NNIRVNAISA GPIKTLASSA IGDFSTMLKS HAATAPLKRN TTQEDVGGAA VYLFSELSKG 250 260 VTGEIHYVDC GYNIMGSTKL

References

[1]	"Mechanisms of evolution in Rickettsia conorii and R. prowazekii." Ogata H., Audic S., Renesto-Audiffren P., Fournier P.-E., Barbe V., Samson D., Roux V., Cossart P., Weissenbach J., Claverie J.-M., Raoult D. Science 293:2093-2098(2001) [PubMed: 11557893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-613 / Malish 7.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006914 Genomic DNA. Translation: AAL03032.1.
PIR	F97761.
RefSeq	NP_360131.1. NC_003103.1.
3D structure databases
ProteinModelPortal	Q92IC6.
SMR	Q92IC6. Positions 4-258.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	927617.
GenomeReviews	Gene locus RC0494 in contig AE006914_GR.
KEGG	rco:RC0494.
NMPDR	fig|272944.1.peg.494.
PATRIC	17888224. VBIRicCon45613_0565.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	MGVANNR.
ProtClustDB	PRK06603.
Enzyme and pathway databases
BioCyc	RCON272944:RC0494-MONOMER.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR014358. Enoyl-ACP_Rdtase_NADH. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00208.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PIRSF	PIRSF000094. Enoyl-ACP_rdct. 1 hit.
PRINTS	PR00081. GDHRDH.
ProtoNet	Search...

Entry information

Entry name

FABI_RICCN

Accession

Primary (citable) accession number: Q92IC6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	December 1, 2001
Last modified:	January 25, 2012
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program